Detection of CD28/CD86 co ‐stimulatory molecules and surface properties of T and dendritic cells: An AFM study

Summary Although the importance of B7/CD28 co‐stimulation has been widely studied, little is known about their nano‐spatial localization and their corresponding cells' biophysical and biomechanical properties. Here, we investigated the morphological, biophysical, and biomechanical properties of T cells and dendritic cells (DCs) by atomic force microscopy (AFM) and force curves. The nano‐spatial distribution of CD28 and CD86 antigen on T cells and DCs was detected by CD86 or CD28 antibody‐functionalized AFM tip. Single‐molecule force spectroscopy (SMFS)‐based force volumes and quantum dots (QDs)‐based fluorescence imaging demonstrated that the co‐stimulatory molecules were not randomly distributed over the cells' surface, but more than 80% of CD28 and CD86 molecules appeared to be expressed as 100–200 nm nanoclusters and polarize dominantly in the peak of the cell membrane fluctuations. AFM imaging and quantitative analysis showed that the roughness of mature DCs (mDCs) was higher than that of immature DCs (iDCs). The adhesion force distribution of iDCs and mDCs was heterogeneous while the elasticity distribution was homogeneous locally. In addition, mDCs had a fourfold increase of Young's modulus of iDCs, indicating the contribution of the actin cytoskeleton to the elastic properties of the cells. Taken together, the nano‐cluster distribution of CD28 and CD86, the rough mDCs surface, the higher adhesion force and elasticity of mDCs may facilitate to the ...
Source: Scanning - Category: Radiology Authors: Tags: Original Article Source Type: research