Roumanian Archives of Microbiology and Immunology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Seroprevalence of antibodies to diphtheria, tetanus and pertussis among healthy adolescents and adults in Iran.
In this study we evaluated the serologic levels of antibodies to diphtheria, tetanus, and pertussis. In a cross sectional study, a total of 360 people aged 10-25 years were randomly selected and classified by sex and age (10-14, 15-20, 21-25 years). Overall, 78.8% of people aged 10-25 years had fully protected levels of diphtheria antibody (> or = 0.1 IU/ML), and 89.7% had fully protected levels of tetanus antibody (> or = 0.1 IU/ML), 94.3% of women aged 15-25 years had anti tetanus antibody sufficient to protect against neonatal tetanus (> or = 0.1 IU/ML). Antibodies to Pertussis toxin (PT) were found in 44.2% sa...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Resveratrol modulates apoptosis in 5-fluorouracyl treated colon cancer cell lines.
Authors: Hotnog D, Mihăilă M, Lancu IV, Matei GG, Hotnog C, Anton G, Bostan M, Braşoveanu LI Abstract Since cancer is a cellular disease, it is essential to identify the development stages and use the information in the prediction, prevention, early detection and design of drug targets. Colon cancer represents a malignancy with high incidence and mortality throughout the world, its etiology involving many genetic, immunological and biochemical factors. 5-fluorouracyl (5-FU) is one of the most effective anti-cancer agents used in the treatment of colorectal cancers, but tumor chemoresistance is a major l...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Escherichia coli ST131 causing invasive infections in Romanian patients--a threat we can no longer ignore.
Authors: Usein CR, Condei M, Cristea D, Ciontea SA, Tatu-Chiţoiu D, Cristea V, Străuţ M Abstract Escherichia coli sequence type ST131 is a major pandemic clonal group of drug-resistant extraintestinal pathogenic E. coli (ExPEC) involved in community-onset and healthcare-associated infections. Thus far, its presence in our area has been paid little attention. This is a preliminary study intended to detect ST131 among 87 clinical isolates retrieved from a larger and unpublished E. coli collection. The study isolates originated from various specimens associated with invasive infections (blood, deep surgica...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Molecular detection of nine antibiotic resistance genes in methicillin resistant Staphylococcus aureus isolates.
This study aimed to evaluate the relation between the phenotypic antibiotic susceptibility patterns and the antibiotic resistance genes and to investigate the prevalence of macrolide, lincosamides, streptogramin, aminoglycoside and tetracycline resistance genes among MRSA isolates. A total of 55 clinical MRSA isolates were included in this study, antibiotic resistance was conducted by Kirby-Bauer disk diffusion method, broth microdilution assay and multiplex PCR technique. Our results showed that there was no discordance between conventional susceptibility testing and gene detection by multiplex PCR assay. The prevalence o...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Seroprevalence study of anti diphtheria antibodies in two age-groups of Romanian adults.
Authors: Dragomirescu CC, Coldea IL, Ilie A, Stănescu A, Ungureanu V, Popa MI Abstract BACKGROUND: Diphtheria represents a serious infectious disease with high epidemic potential. It is a vaccine preventable disease (a minimum vaccine coverage of 95% for children of 1 year and 90% in adults could prevent the disease). Diphtheria vaccination is included in the National Immunization Program (NIP). Complete vaccination for children consists in DTaP (diphtheria toxoid, tetanus toxoid and acellular pertussis vaccine) vaccine administration from the age of 2 months until 4 years and dT vaccine (tetanus toxoid a...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Differentiation of influenza B lineages from clinical samples by one-step real-time RT-PCR.
CONCLUSION: Differentiation of genetic lineage B influenza virus circulating in Romania in the next seasons by one-step real-time RT-PCR method will supplement the classical test, haemagglutination inhibition (HI), which requires growing of the virus. This method can be advantageous for a balanced selection of samples, in case of lineages co-circulation, for genetic and antigenic characterization. PMID: 25518567 [PubMed - indexed for MEDLINE] (Source: Roumanian Archives of Microbiology and Immunology)
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Hematology references for three laboratory mice strains.
Authors: Marinescu B, Isvoranu G, Anghelache L, Cionca F Abstract The paper contains reference data for the main haematologic indicators from three strains of conventional mice: Balb/c, C57BL/6 and CD1. These data listed include the mean and the standard deviation of the studied values. The animals used were divided by sex (sex ratio 1:1) and age. The study was performed in standard conditions. These strains were chosen as they are widely used for preclinical evaluations. Therefore, our data are expected to be useful for investigators conducting qualitative and quantitative toxicity studies in these mice. ...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Antimicrobial and antioxidant activities of alcoholic extracts obtained from vegetative organs of A. retroflexus.
Authors: Marinaş IC, Chifiriuc C, Oprea E, Lazăr V Abstract In vitro antimicrobial and antioxidant activities of Amaranthus retroflexus leaves and inflorescence alcoholic (ethanol 70%) extracts of various concentrations ranging from 0.78 to 400 μL/ml were analyzed on different clinical and reference bacterial strains (Staphylococcus aureus, Bacillus subtills, Enterococcus faecalis, Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumannii) and fungal strains (Candida albicans, C. famata, C. utilis, Saccharomyces cerevisiae) using agar disk diffusion method and broth diluti...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

KIR genotyping in the selected population in Andhra Pradesh (India).
Authors: Vaishnav G, Krupanidhi S, Sanjeevi CB Abstract BACKGROUND: The population is not always homogeneous in relation to the representation and functioning of genes. Therefore, the presence of allogenicity is a universal phenomenon. The profound variability is noticed among the members of the human population with reference to the resistance against infections and late onset of diseases. In this line, a few sets of alleles which come under the domain of immune function namely KIRs (Killer immunoglobulin-like receptor genes) and HLA-I have been chosen to report in the population of Puttaparthi (India). ...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Immunological investigations in prostatic pathology--a prospective study.
Authors: Spînu D, Mischianu D, Surcel M, Huică R, Munteanu A, Pîrvu I, Ciotaru D, Bratu O, Farcaş C, Manache Ş, Ursaciuc C Abstract BACKGROUND: BPH with prostatitis represents one of the most common urological pathologies affecting most men. The etiology of both conditions remains at the discretion of the various assumptions. OBJECTIVES: The body's cellular immune response in prostate adenoma is a less studied aspect which we have focused on, in this paper. The correlation with a wide range of information from specific investigations such as prostate-specific antigen (PSA) and total histopatho...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Status of immunity against poliomyelitis in the acute flaccid paralysis (AFP) cases in Romania between 2009-2012.
Authors: Băicuş A Abstract Poliovirus (PV), a member of the Enterovirus genus, is the etiological agent of poliomyelitis, an acute paralytic disease. No poliovirus strain has been isolated in Romania since 2009. A serosurvey study carried out between 2009-2012, that involved 76 serum samples from acute flaccid paralysis (AFP) cases, showed a low protection level in the examined subjects against type 1 (80%), type 2 (79%) and type 3 (71%) poliovirus Sabin strains. Samples with titers ≥ 1:8 were considered positive. Suboptimal seroprevalence in the AFP cases confirmed that a high level of immunization ag...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Is there a difference in the incidence of helicobacter pylori infection in patients with some chronic diseases?
IS THERE A DIFFERENCE IN THE INCIDENCE OF HELICOBACTER PYLORI INFECTION IN PATIENTS WITH SOME CHRONIC DISEASES? Roum Arch Microbiol Immunol. 2014 Jul-Dec;73(3-4):65-8 Authors: Constantinescu CA, Constantinescu EM Abstract Globally, Helicobacter pylori infection is estimated to be present in two-thirds of the population. This longitudinal, observational, comparative case-control study conducted in the county of Braşov on 763 subjects split into two groups: one with Helicobacter pylori infection (412 people) and the other without infection (351 people) allowed us to compare our res...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Serum alpha fetoprotein, a surrogate marker for liver disease progression in chronic hepatitis c.
This study aims to evaluate the value of AFP as an early predictor of the evolution of chronic hepatitis C. In a retrospective study on 116 HCV-infected patients (62.9% females, mean age 49.13 ± 1.73 years), increased levels of serum AFP (> 7 ng/mL) were found in 39.7% of cases. High serum AFP levels were more frequently detected in older patients and in those with severe fibrosis and cirrhosis (62.2% and 76.9% respectively vs. 11.6% in those without significant fibrosis, p = 0.0001). Increased AFP levels were significantly associated with markers of hepatic cytolysis (ALT- r = 0.245, p = 0.009 and AST r = 0.441, p = 0...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Molecular characterization of staphylococcus aureus strains isolated from infective endocarditis.
In this study we analyzed the virulence profile, including adhesins, exotoxins, superantigens and biofilm determinants, along with agr type detection, for S. aureus strains isolated from IE, versus non-IE originating strains. We performed also bacterial typing (SCCmec typing, spa-typing and MLST typing), in order to compare our strains with international databases repositories. Although the study was carried out on a reduced number of isolates, our observations confirm the previous works, showing that no major differences were observed between the genetic backgrounds of the two groups of strains analyzed. Notably, the adde...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Microrna biogenesis and its role in hiv-1 infection.
MICRORNA BIOGENESIS AND ITS ROLE IN HIV-1 INFECTION. Roum Arch Microbiol Immunol. 2014 Jul-Dec;73(3-4):84-91 Authors: Roşca A, Anton G, Ruţă S Abstract MicroRNA (miRNA) are small- 19-24 nucleotides, non-coding RNA molecules that regulate translational and post-translational processes through mRNA degradation and protein translation repression, or sometimes through heterochromatin formation or activation of protein translation. Lately, miRNA are investigated as predictive biomarkers for the evolution and prognosis of viral diseases, as well as therapeutic targets. Although the r...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research