Roumanian Archives of Microbiology and Immunology 
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This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.The inhibitory activity of pomelo essential oil on the bacterial biofilms development on soft contact lenses.
Authors: Saviuc C, Dascălu L, Chifiriuc MC, Rădulescu V, Oprea E, Popa M, Hristu R, Stanciu G, Lazăr V
Abstract
The aim of present study was to investigate the microbial colonization of worn contact lenses (CLs) and to evaluate the inhibitory effect of pomelo (Citrus maxima) peels essential oil on the biofilm development on unworn CLs. The essential oil was isolated by steam distillation and analyzed by gas chromatography coupled with mass spectrometry, twenty compounds being isolated. The antimicrobial activity of pomelo oil was tested against S. epidermidis and P. aeruginosa strains, known for their a...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research
Tracking down of laryngo-pharyngeal metastasis.
CONCLUSION: Our results sustain that the detection of HPV DNA in blood is a "surrogate marker" of metastasis when extension of metastasis cannot be estimated, this observation is very important for management of cancer patients with laryngopharyngeal localization.
PMID: 21434592 [PubMed - indexed for MEDLINE] (Source: Roumanian Archives of Microbiology and Immunology)
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research
Cytokine profiles of HeLa and human diploid cells induced by different fractions of Vibrio parahaemolyticus cultures exposed to stress conditions.
Authors: Chifiriuc MC, Bleotu C, Pîrcălăbioru G, Israil AM, Dinu S, Rută SM, Grancea C, Lazăr V
Abstract
Vibrio (V.) parahaemolyticus is an aquatic halophilic bacteria which produces gastroenteritis and in rare cases septicaemia after the consumption of raw or under-cooked contaminated seafood.The severity of diarrheal illness caused by this bacterium is closely related to the presence of two types of hemolysins (the thermostable direct hemolysin-TDH and TDH related hemolysin-TRH) and also of type III secretion system (TTSS) proteins. The TTSS type 1 induces a wide array of effects on infected HeLa ce...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research
IgG-F-actin antibodies in celiac disease and dermatitis herpetiformis.
Authors: Samaşca G, Băican A, Pop T, Pîrvan A, Miu N, Andreica M, Cristea V, Dejica D
Abstract
Anti-actin antibodies are found in 52-85% of patients with autoimmune hepatitis or chronic active hepatitis and in 22% of patients with primary biliary cirrhosis. In patients with celiac disease, anti-actin antibodies correlate with the degree of villous atrophy. Studies on their involvement in celiac disease and dermatitis herpetiformis in Romania have not been done. The purpose of this study was to evaluate of the quality of IgG anti-F-actin antibodies (IgG-AAA) tests compared with IgA tissue transglutaminas...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research
Proinflammatory cytokines: an insight into pancreatic oncogenesis.
Authors: Miron N, Miron MM, Milea VG, Cristea V
Abstract
Pancreatic cancer is a highly lethal disease, being one of the five leading death causes among oncologic patients. It is usually diagnosed late due to the paucity of clinical signs, and the current therapy means have limited success. One of the documented risk factors for developing pancreatic adenocarcinoma is chronic pancreatitis. It is postulated that a chronic inflammatory disease has a potential of evolving toward neoplasia, a fact that could account for a percentage of the pancreatic cancers. Starting from this assumption, we intended to analyz...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research
Plasticity of regulatory T cells under cytokine pressure.
Authors: Diaconu CC, Neagu AI, Lungu R, Tardei G, Alexiu I, Bleotu C, Economescu MC, Bumbăcea RS, Pele I, Bumbăcea D
Abstract
CD4+ T helper (Th) cells have been divided into different subsets as defined by their cytokine products and functions after their activation. CD4+ T cell subsets are continuously discovered and until now Th1, Th2, Th9, Th17, and regulatory T (Treg) cells have been almost unanimously recognized but yet not completely characterized. The selective production of cytokines by each of the subsets is probably the master key of the mechanisms of immune regulation. The cytokine milieu is e...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research
A multiple typing approach is recommended for the laboratory-based surveillance of salmonellosis in Romania.
Authors: Usein CR, Străuţ M, Palade AM, Tatu-Chiţoiu D, Ciontea SA, Băltoiu MC, Dinu S, Damian M
Abstract
In Romania, Salmonella enterica serovar Typhimurium isolates are currently typed by antimicrobial resistance profiles and phage typing, as part of the national laboratory-based surveillance system of human enteric infections. The aim of the present study was to assess the added value of complementing this approach with molecular fingerprinting, namely pulsed-field gel electrophoresis (PFGE) and multiple-locus variable-number tandem-repeats analysis (MLVA). Thirty-six S. Typhimurium isolates receive...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research
Antimicrobial activity of copper and silver nanofilms on nosocomial bacterial species.
Authors: Codiţă I, Caplan DM, Drăgulescu EC, Lixandru BE, Coldea IL, Dragomirescu CC, Surdu-Bob C, Bădulescu M
Abstract
Contaminated surfaces are possible vehicles in infection transmission. It is known that both Copper (Cu) and Silver (Ag) efficiently inactivate microbes by direct contact. Aiming at using these metals for benefitting from their antimicrobial effect, but to avoid subsequent toxic effects, we evaluated the antimicrobial activity of nanometric thin Silver and Copper films covering less expensive materials. Using a modified version of the Japan Industrial Standard JIS Z 2801:2000, we demo...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research
Quantitative real-time PCR study of the influence of probiotic culture soluble fraction on the expression of Pseudomonas aeruginosa quorum sensing genes.
In conclusion, these results proved that the inhibition of virulence factors regulation mechanisms by soluble molecules secreted by probiotics could represent an interesting way pathogenicity and virulence attenuation in Ps. aeruginosa nosocomial strains.
PMID: 21462836 [PubMed - indexed for MEDLINE] (Source: Roumanian Archives of Microbiology and Immunology)
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research
Antimicrobial activity of plant essential oils against bacterial and fungal species involved in food poisoning and/or food decay.
Authors: Lixandru BE, Drăcea NO, Dragomirescu CC, Drăgulescu EC, Coldea IL, Anton L, Dobre E, Rovinaru C, Codiţă I
Abstract
The currative properties of aromatic and medicinal plants have been recognized since ancient times and, more recently, the antimicrobial activity of plant essential oils has been used in several applications, including food preservation. The purpose of this study was to create directly comparable, quantitative data on the antimicrobial activity of some plant essential oils prepared in the National Institute of Research-Development for Chemistry and Petrochemistry, Bucharest to be ...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research
Mitochondrial DNA mutations in patients with HRHPV-related cervical lesions.
Authors: Goia-Ruşanu CD, Iancu IV, Botezatu A, Socolov D, Huică I, Pleşa A, Anton G
Abstract
High risk human papillomaviruses (hr-HPV) are known to be the etiological agents of cervical cancer disease. On the other hand, other cofactors are considered to be important in cervix carcinogenesis. Mutations in mitochondrial DNA (mtDNA) as well as alterations in mtDNA content have been reported in numerous cancers examined to date. The D-loop region has been shown to be a mutational "hot spot" in human cancer. In order to evaluate the role of mtDNA mutations in cervical lesions progression, cervical specimens...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research
In vitro antimicrobial activity of Romanian medicinal plants hydroalcoholic extracts on planktonic and adhered cells.
Authors: Stanciuc AM, Gaspar A, Moldovan L, Saviuc C, Popa M, Măruţescu L
Abstract
The aim of this study was to assess the antibacterial and antifungal potential of some Romanian medicinal plants, arnica--Arnica montana, wormwood--Artemisia absinthium and nettle--Urtica dioica. In order to perform this antimicrobial screening, we obtained the vegetal extracts and we tested them on a series of Gram-positive and Gram-negative bacteria, and also against two fungal strains. The vegetal extracts showed antimicrobial activity preferentially directed against the planktonic fungal and bacterial growth, while the...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research
Assessing the efficiency of free light chain assay in monitoring patients with multiple myeloma before and after autologous stem cell transplantation along with serum protein electrophoresis and serum protein immunofixation.
In this study we have presented the dynamic evolution of 7 patients with intact immunoglobulin multiple myeloma (IIMM) (2 IgG, kapa; 3 IgG, lambda; 1 IgA, kappa; 1 IgA, lambda) and 2 patients with light chain multiple myeloma before and after autologous peripheral blood stem cell transplantation (PBSCT). All 7 patients fulfilled the four criteria for the diagnosis of IIMM: bone marrow plasma cells exceeding 20%, lytic bone lesions, identification and quantification of M protein by scanning densitometry of electrophoresis gels, IFE (immunofixation protein electrophoresis) confirmed and typed the M protein. All patients had ...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research
Immunodetection of added glycomacropeptide in milk formulas and milk powders.
Authors: Oancea S, Stoia M
Abstract
The present study aimed the detection of fraudulent manipulation of milk powder with a low cost component--whey powder, by applying the immunochromatographic assay to identify glycomacropeptide. Five commercial milk powder samples of various brands from the national market were analyzed: lactose enriched milk powder type 26, two whole milk powders, vitamin enriched milk powder and full cream milk powder. Our results showed additional whey (1-2%) in 60% of the selected samples after casein removal by precipitation with 20% trichloracetic acid. Another investigated sample-...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research
Bacterial extract cantastim activates macrophages via TLR-2.
Authors: Caraş I, Tucureanu C, Pitica R, Sălăgeanu A
Abstract
CANTASTIM is a second generation bacterial immunomodulator. The aim of this study was to examine the mechanism by which bacterial immunomodulator CANTASTIM induces production of inflammatory cytokines in monocytes/macrophages. Proinflammatory cytokines were induced in PMA-differentiated THP-1 cells by stimulation with TLR agonists and CANTASTIM in the presence or absence of anti-TLR blocking antibodies or isotype matched control antibodies. Also, RNA interference was used to knockdown TLR2 or TLR4 expression in PMA-differentiated THP-1 cells ...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research
