Roumanian Archives of Microbiology and Immunology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Double transgenic mice--a suitable model for studying oxidative stress in type 1 diabetes mellitus.
Authors: Iancu AD, Stăvaru C Abstract Type 1 diabetes mellitus (T1DM), one of the most prevalent chronic diseases is characterized by the progressive destruction of pancreatic beta cells, leading to insulin deficiency and hyperglycemia. Studies performed on diabetic subjects with prolonged hyperglycemia showed the oxidative stress occurrence followed by molecular, cellular and tissue damage. Currently, reducing the oxidative stress represents a therapeutic target, in order to reduce its complications in diabetic patients. An adequate experimental model of type 1 diabetes represents a prerequisite in oxida...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Capsaicin short term administration effect on different immune parameters.
This study was based on the assumption that intraperitoneal administration of a low dose of capsaicin, on a short period of time, can decrease pain sensations generation and transmission. If from the neurological point of view, capsaicin effects are known, its effects on the immune system are not clear yet. Therefore, in this study we have investigated capsaicin effects on oxygen and nitrogen free radicals generation by phagocytic immune cells, in lymphocyte populations, and also capsaicin effects on plasmatic protein oxidation. Our results point to minor modifications in oxygen reactive species production, simultaneous wi...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Understanding the molecular targets for new therapeutical agents in hepatitis c infection.
Authors: Vagu C, Sultana C, Ruţă S Abstract Improved understanding of the HCV viral life cycle has led to the identification of numerous potential molecular targets for the development of new drugs. Direct acting antivirals -DAAs specifically target a viral encoded protein: the NS3-4A protease, involved in the posttranslational viral protein processing; the NS5B encoded viral polymerase, that conducts the nucleic acid replication and the NS5A encoded phosphoprotein, that participates in both replication and virus assembly. Host-targeted agents, both directed to the early steps of viral replication (recep...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Growth of Entamoeba invadens in sediments with metabolically repressed bacteria leads to multicellularity and redefinition of the amoebic cell system.
Authors: Niculescu VF Abstract Extracellular signaling and mechanisms of cell differentiation in Entamoeba are misunderstood. The main reason is the popular use of axenic media, which do not correspond to the natural habitats of Entamoeba. The axenic environment lacks the exogenous activators and repressors provided by natural habitats. Absent bacterial commensals understanding of the development of the amoebic cell system remains deficient. The present Aa(Sm) culture method using mixed sediments of antibiotically repressed Aerobacter aerogens and amoebae was developed to model in vitro extracellular signa...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Two case reports on visceral leishmaniasis diagnosed in Romania.
Authors: Gogoaşe MG, Teodorescu I, Preda C, Ionescu SC Abstract Two cases of visceral leishmaniasis with a species from Leishmania donovani complex were detected in the Fundeni Clinical Institute. In one case, two infection sources were possible: one from Italy, where the patient worked three years, the other from the Southwest of Romania (Dolj County), where he was resident and where few human and canine leishmaniasis cases were registered in the past. In the second case, the patient lived in the Northeast of Romania (Iaşi County), but worked in the same southwest zone. In both cases, a local transmissi...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Adenylate cyclases involvement in pathogenicity, a minireview.
Authors: Costache A, Bucurenci N, Onu A Abstract Cyclic AMP (cAMP), one of the most important secondary messengers, is produced by adenylate cyclase (AC) from adenosine triphosphate (ATP). AC is a widespread enzyme, being present both in prokaryotes and eukaryotes. Although they have the same enzymatic activity (ATP cyclization), the structure of these proteins varies, depending on their function and the producing organism. Some pathogenic bacteria utilize these enzymes as toxins which interact with calmodulin (or another eukaryote activator), causing intense cAMP synthesis and disruption of infected cell ...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

The effectiveness of cytological rescreening in the reduction of false negative/positive Pap reports.
CONCLUSION: Partial random rescreening or targeted rescreening enables a better interpretation of suboptimal prepared smears. Targeted rescreening allows a correct detection of even low percentages of atypical cells. Other confounding factors, such as the laboratory workload and the regional prevalence of the disease, can exert an important effect on the correct classification of cytological lesions. PMID: 24187808 [PubMed - indexed for MEDLINE] (Source: Roumanian Archives of Microbiology and Immunology)
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Thin layer chromatography characterization of ELISA specific glycolipids antigens immunomagnetically purified from environmental mycobacteria.
Authors: Ulea I, Stavri H Abstract Thin layer chromatography (TLC) could easily and rapidly evidentiate the qualitative differences between glycolipids (GLs). Different immunomagnetically purified mycobacterial GLs have been compared using TLC, in order to choose the most appropriate antigens to be utilized in ELISA. The GLs were purified from environmental mycobacteria (EM) (M. avium-intracellulare, M. kansasii, M. xenopi, M. scrofulaceum and M. gordonae) and from M. tuberculosis H37Rv. BioMag Amine and BioMag Carboxyl terminated superparamagnetic microparticles were utilized in the magnetic separation of...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

HIV-1 circulating subtypes in Romania.
This article reviews the hypothesis on the origin and the unusual steady persistence of this HIV strain and discusses the recent changes in the molecular epidemiology of the epidemic, associated to the emergence of new infection routes. Phylogenetic and phylogeography studies conducted through the epidemic seem to indicate that HIV F subtype originated in the 1950s in the Democratic Republic of Congo and was separately spread by immigration waves to Brazil, Angola and Romania. Data released at the end of 2012 report F1 subtype as the dominant HIV-1 clade in Romania in all categories of patients: recently infected or late p...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Clinical and diagnosis considerations of Lyme disease.
Authors: Ionescu D, Cotar AI, Bădescu D, Dumitriu S PMID: 24187811 [PubMed - indexed for MEDLINE] (Source: Roumanian Archives of Microbiology and Immunology)
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Professor Ion Cantacuzino--tribute to the 150th anniversary of his birth.
Authors: Neguţ M PMID: 24597172 [PubMed - indexed for MEDLINE] (Source: Roumanian Archives of Microbiology and Immunology)
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Phenotypic and genotypic characterization of Staphylococcus aureus strains isolated from a familial foodborne outbreak.
Authors: Coldea IL, Drăgulescu EC, Lixandru BE, Dragomirescu CC, Codiţă I Abstract Although Staphylococcus aureus is frequently reported among the common causative agents of foodborne diseases in Europe, very little is known about the strains involved in staphylococcal food-poisoning outbreaks in our region. Therefore, the aim of this study was to characterize the staphylococcal strains recovered from an autochthonous food-poisoning familial outbreak through phenotypic and genotypic methods. Ten S. aureus strains from food and human sources, submitted to the reference laboratory, were tested for suscept...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Phenotypic profiles of virulence in different Candida species isolated from vulvovaginal infections.
Authors: Sârbu I, Pelinescu D, Stoica I, Măruţescu L, Vassu T Abstract In order to establish an infection, pathogenic microorganisms have to colonize, survive, multiply, evade the immune system and spread to other tissues [1, 2, 3]. Although some Candida species are normally commensal in humans, in the last decades the frequency and the severity of nosocomial diseases due to Candida strains have increased dramatically [4]. The aim of the present study was to characterize some Candida strains isolated from vulvovaginal infections by determining the virulence and pathogenicity profile. The assessment of t...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Selection of bacterial strains efficient in decolorization of remazol black-B.
Authors: Shah MP, Sebastian S, Mathukiya HM, Darji AM, Patel J, Patel K Abstract Azo dyes are released into wastewater streams without any pretreatment and polluted water and soil environments. To prevent contamination of our vulnerable resources, removal of these dye pollutants is of great importance. For this purpose, wastewater samples were collected from dye-contaminated sites of Ankleshwar, Gujarat, India. About 50 bacterial isolates were isolated through enrichment and then tested for their potential to remove Remazol Black-B azo dye in liquid medium. Three bacterial isolates capable of degrading Rem...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Single and multipathogen viral infections in hospitalized children with acute respiratory infections.
Authors: Tecu C, Mihai ME, Alexandrescu VI, Orăşeanu D, Zapucioiu C, Ivanciuc AE, Necula G, Lupulescu E, Chiriţă D, Piţigoi D Abstract We aimed to describe the viral etiology of acute respiratory tract infections in children aged 0-8 years admitted to Grigore Alexandrescu Hospital, the largest pediatric hospital in Romania. The patients had clinical diagnosis of pneumonia, bronchiolitis or viral respiratory infections and had been hospitalized between September 2010 and September 2011. The study was part of the "Molecular investigations of acute respiratory infections caused by non-influenza viruses, ...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research