Roumanian Archives of Microbiology and Immunology 
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This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.Identification of four Treponema species in subgingival samples by nested-PCR and their correlation with clinical diagnosis.
Authors: Dabu B, Mironiuc-Cureu M, Jardan D, Szmal C, Dumitriu S
Abstract
The relationship between different species of oral Treponemas and inflammation in periodontal disease progression is complex. The purpose of this study was to analyze and compare the subgingival plaque samples collected from periodontally healthy subjects and from chronic gingivitis and periodontitis patients in order to detect the presence of T. denticola, T. pectinovorum, T. socranskii and T. vincentii using nested-PCR technology. After DNA extraction from the samples using QIAmp DNA Mini Kit (QIAGEN, the four Treponema species wer...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research
Etiology of pneumonia in children in the absence of pneumococcal and antihaemophilus vaccines.
Authors: Sur G, Kudor-Szabadi L, Vidrean V, Samaşca G
Abstract
Childhood pneumonia represents an important pathology, a cause of morbidity and mortality worldwide. Our study aims to determine etiology of pneumonia in hospitalized children using several laboratory methods. We performed a prospective study that enrolled 560 children age 1 up to 18 years old all diagnosed with pneumonia by clinical and radiological features. We applied various laboratory methods (serologic, bacteriologic: bronchial aspirate, sputum, pleural effusion and blood culture) in order to identify a pathogen agent that caused pneumon...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research
Prevalence of hepatitis B virus in chronic hepatitis B patients.
Authors: Sadeghifard N, Mohebi R, Sekawi Z, Ghafourian S, Kazemian H, Rezaee M
Abstract
The aim of the current study was to detect HBV by Real time - PCR in chronic hepatitis B patients. Fifty-eight sera of chronic hepatitis B patients were subjected during the period March 2009 to April 2010 in Ilam cities in West of Iran. Sera assayed by real-time PCR and ELISA methods. Twenty serum samples from healthy volunteers and non-hepatitis B patients and negative for hepatitis B seromarkers served as negative controls for the study. Among fifty-eight sera, ELISA showed fifty-five (94.8%) of the samples were posi...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research
In vitro antimicrobial and antioxidant activity of black thyme (Thymbra spicata L.) essential oils.
Authors: Saidi M, Ghafourian S, Zarin-Abaadi M, Movahedi K, Sadeghifard N
Abstract
In order to study antimicrobial effects of essential oils of Wild Thyme (Thymbra spicata L.) on two Gram positive bacteria (Streptococcus agalactiae RJTTC1978 and Staphylococcus aureus RJTTC1885) and two Gram negative bacteria (Escherichia coli RJTTC2409 and Klebsiella pneumoniae RJTTC1097), the research carried out with five concentrations (0.2, 2, 4, 10 and 20 microl) using disk diffusion and microbroth dilution (to determine MIC and MBC) methods at Ilam University during 2010. Tetracycline and Gentamicin discs were used a...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research
In vitro antimicrobial activity of Persian shallot (Allium hirtifolium).
In this study, the antimicrobial activity of Persian shallot hydroalcoholic extract and F1 fraction of the plant (containing amino acid derivatives and/or other cationic compounds) was investigated on some Gram positive cocci and bacilli, Gram negative bacilli, two protozoa, a yeast and a fungus. Excellent activity against Candida albicans (MIC = 64 microg/ml, MBC = 128 microg/ml), Leishmania infantum (MIC = 0.2 mg/ml on the first day of study) and Trichomonas vaginalis (MIC = 5 microg/ml in PSDE form) and a moderate activity against Bacillus spp and Pseudomonas aeroginosa (MIC = 128 microg/ml) was observed. The results sh...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research
Antibiotic resistance profiles of Acinetobacter sp. strains isolated from intensive-care unit patients.
CONCLUSIONS: Our results confirmed that the multidrug resistance is the major threat of Acinetobacter sp. infections, especially when they occur in high risk patients.
PMID: 23210320 [PubMed - indexed for MEDLINE] (Source: Roumanian Archives of Microbiology and Immunology)
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research
Characterization of Klebsiella pneumoniae strains producing extended spectrum beta-lactamases and AMPC type beta-lactamases isolated from hospitalized patients in Kerman, Iran.
Authors: Mansouri S, Kalantar D, Asadollahi P, Taherikalani M, Emaneini M
Abstract
Klebsiella pneumoniae is a major cause of nosocomial infections. Emergence of antibacterial resistance and production of beta-lactamases are responsible for the frequently observed empirical therapy failures. The aim of this study was to determine the presence and the prevalence of extended spectrum beta-lactamases (ESBLs) and AmpC beta-lactamases in clinical isolates of K. pneumoniae in Kerman, Iran. Resistance to different antibiotics was determined using standard disk diffusion method. The beta-lactamases phenotypes were ...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research
Bloodstream infections in immunocompromised hosts.
Authors: Papagheorghe R
Abstract
Immunocompromised hosts are at high risk for bloodstream infections (BSIs); the outcome is unpredictable (even with adequate therapy), frequently severe (mortality 27%). A 26 months survey in a haematology/oncology hospital was set-up; it comprised 158 patients and detected 171 positive blood cultures. The origin of the infections was primitive in 27.22% and secondary in 72.78% of the cases; the most frequent cause was the presence of a vascular catheter (23.41%). The second most important cause were the respiratory infections (19.62%), followed by the urinary tract infecti...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research
Immunological manifestations in type I diabetic children.
Authors: Răchişan AL, David BA, Căinap S, Miu N, Andreica M, Samaşca G
Abstract
In the past decade a number of studies suggested that type 1 diabetes mellitus is an oxidative stress influenced disease. Paraoxonase 1 enzyme plays a crucial role in antiatherogenic-antioxidant circle. The aim of our study was to examine the possible differences in paraoxonase 1 enzymatic activities in diabetic children associated other autoimmune diseases versus a control group. Another objective of the study was to determine if there is any difference according to the gender in paraoxonase 1 activities (arylesterase and ...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research
Impact of the molecular diagnosis on the management of patients with aseptic meningitis.
Authors: Băicuş A, Cardaş R
Abstract
Between 2007-2008, epidemic episodes of aseptic meningitis occurred in Romania. Most important strains isolated were Echoviruses. A commercial kit for rapid molecular detection of aetiological agents of aseptic meningitis was tested. The introduction of the molecular tests in clinical use is important by the efficient decision-making concerning the treatment.
PMID: 23210324 [PubMed - indexed for MEDLINE] (Source: Roumanian Archives of Microbiology and Immunology)
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research
Abstracts of a conference: Diaspora in Romanian Scientific Research and Education, September 25-28, 2012, Bucharest, Romania.
Authors:
PMID: 23236654 [PubMed - indexed for MEDLINE] (Source: Roumanian Archives of Microbiology and Immunology)
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research
George Emil Palade centenary (1912-2012).
Authors: Cernescu C
PMID: 23755697 [PubMed - indexed for MEDLINE] (Source: Roumanian Archives of Microbiology and Immunology)
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research
Considerations on the isolation of Listeria monocytogenes and other Listeria spp. in food products.
Authors: Borcan AM, Caplan DM
Abstract
Listeria monocytogenes, a Gram positive bacillus, is well adapted for survival as a saprophyte in soil and decaying vegetation, but also able to cause serious infections, due to its ability of intracellular multiplication and meningeal and placental dissemination. A total number of 43 Listeria spp. strains isolated from food were investigated. Bacterial identification was performed according to standard methodology, based on: Gram staining affinity, morphology, culture aspects on blood agar (examined in oblique light), catalase test, beta/hemolysis (on sheep blood aga...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research
Comparative analysis of biofilm development among MRSA and MSSA strains.
Authors: Ghafourian S, Mohebi R, Rezaei M, Raftari M, Sekawi Z, Kazemian H, Mohseni A, Karimi S, Sadeghifard N
Abstract
As the recalcitrance of biofilm-mediated infections to the anti-infective treatment has an adverse effect on patient's health, the main objective of this study was to investigate the capacity of clinical isolates of Staphylococcus aureus with different resistance patterns to form biofilms. S. aureus strains are among the most representative etiology of infections in the health-care environment of Milad hospital in Iran. The results showed that out of 80 analyzed strains, 27 methicillin re...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research
Correlations among different markers determined by immunochemical methods used for the diagnosis and monitoring of intact immunoglobulin multiple myeloma cases.
In this study 354 samples coming from 46 patients with IIMM were assayed, out of which: 19, IgGkappa; 13, IgGlambda; 7, IgAkappa; 7, IgAlambda. At the diagnosis, immunofixation was positive in all samples and serum protein electrophoresis quantified M- protein for all patients. Free light chains concentrations were abnormal in 92.25% of patients with concentrations above the reference ranges in all patients with IgGkappa and IgAkappa MM. The intact immunoglobulins were elevated in 83.12% of cases. Pearson correlation coefficient showed correlations among the free light chains serum levels (kappa, lambda and ratio kappa/lam...
Source: Roumanian Archives of Microbiology and Immunology - December 12, 2015 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research
