Characterization of Klebsiella pneumoniae strains producing extended spectrum beta-lactamases and AMPC type beta-lactamases isolated from hospitalized patients in Kerman, Iran.

Characterization of Klebsiella pneumoniae strains producing extended spectrum beta-lactamases and AMPC type beta-lactamases isolated from hospitalized patients in Kerman, Iran. Roum Arch Microbiol Immunol. 2012 Apr-Jun;71(2):81-6 Authors: Mansouri S, Kalantar D, Asadollahi P, Taherikalani M, Emaneini M Abstract Klebsiella pneumoniae is a major cause of nosocomial infections. Emergence of antibacterial resistance and production of beta-lactamases are responsible for the frequently observed empirical therapy failures. The aim of this study was to determine the presence and the prevalence of extended spectrum beta-lactamases (ESBLs) and AmpC beta-lactamases in clinical isolates of K. pneumoniae in Kerman, Iran. Resistance to different antibiotics was determined using standard disk diffusion method. The beta-lactamases phenotypes were determined by combined disk method. Polymerase chain reaction (PCR) was used to determine blaCTX-M and blaCMY genes in the ESBLs and AmpC positive isolates. Out of the 75 K. pneumoniae isolates, 31 (41.3%) produced ESBLs, 11 (14.6%) produced AmpC beta-lactamases and 1 (1.3%) was resistant to imipenem, probably by the production of a metallo beta lactamase in the phenotypic assay. Simultaneous production of ESBLs and AmpC beta-lactamases as well as the concomitant presence of blaCTX-M and blacMY genes was detected in one isolate. Prevalence of blaCTX-M and blaCMY among isolates were 20% and 2.6%, respectivel...
Source: Roumanian Archives of Microbiology and Immunology - Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research