miR-200c and phospho-AKT as prognostic factors and mediators of osteosarcoma progression and lung metastasis
Lung metastasis is the major cause of death in osteosarcoma patients. However, molecular mechanisms underlying this metastasis remain poorly understood. To identify key molecules related with pulmonary metastasis of pediatric osteosarcomas, we analyzed high-throughput miRNA expression in a cohort of 11 primary tumors and 15 lung metastases. Results were further validated with an independent cohort of 10 primary tumors and 6 metastases. In parallel, we performed immunohistochemical analysis of activated signaling pathways in 36 primary osteosarcomas. (Source: Molecular Oncology)
Source: Molecular Oncology - April 22, 2016 Category: Cancer & Oncology Authors: Pablo Berlanga, Lisandra Mu ñoz, Marta Piqueras, J. Antoni Sirerol, María Dolors Sánchez-Izquierdo, David Hervás, Miguel Hernández, Margarita Llavador, Isidro Machado, Antonio Llombart-Bosch, Adela Cañete, Victoria Castel, Jaime Font de Mora Source Type: research
miR-200c and phospho-AKT as prognostic factors and mediators of osteosarcoma progression and lung metastasis
Lung metastasis is the major cause of death in patients with osteosarcoma. However, the molecular mechanisms underlying this metastasis remain poorly understood. In order to identify key molecules related with pulmonary metastasis of pediatric osteosarcomas, we analyzed high throughput miRNA expression in a cohort of 26 patient samples (11 primary tumors and 15 lung metastases) and results were further validated with a second, independent cohort of 16 osteosarcomas (10 primary tumors and 6 metastases). (Source: Molecular Oncology)
Source: Molecular Oncology - April 22, 2016 Category: Cancer & Oncology Authors: Pablo Berlanga, Lisandra Muñoz, Marta Piqueras, J. Antoni Sirerol, María Dolors Sánchez-Izquierdo, David Hervás, Miguel Hernández, Margarita Llavador, Isidro Machado, Antonio Llombart-Bosch, Adela Cañete, Victoria Castel, Jaime Font de Mora Source Type: research
Gene expression profiling of single circulating tumor cells in ovarian cancer – Establishment of a multi-marker gene panel
The presence of circulating tumor cells (CTCs) in the blood of ovarian cancer patients was shown to correlate with decreased overall survival, whereby CTCs with epithelial –mesenchymal-transition (EMT) or stem-like traits are supposed to be involved in metastatic progression and recurrence. Thus, investigating the transcriptional profiles of CTCs might help to identify therapy resistant tumor cells and to overcome treatment failure. For this purpose, we established a multi-marker panel for the molecular characterization of single CTCs, detecting epithelial (EpCAM, Muc-1, CK5/7), EMT (N-cadherin, Vimentin, Snai1/2, CD117,...
Source: Molecular Oncology - April 19, 2016 Category: Cancer & Oncology Authors: Christina Blassl, Jan Dominik Kuhlmann, Alessandra Webers, Pauline Wimberger, Tanja Fehm, Hans Neubauer Source Type: research
Gene expression profiling of single circulating tumor cells in ovarian cancer – Establishment of a multi-marker gene panel
The presence of circulating tumor cells (CTCs) in the blood of ovarian cancer patients was shown to correlate with decreased overall survival, whereby CTCs with epithelial –mesenchymal-transition (EMT) or stem-like traits are supposed to be involved in metastatic progression and recurrence. Thus, investigating the transcriptional profiles of CTCs might help to identify therapy resistant tumor cells and to overcome treatment failure. For this purpose, we established a multi-marker panel for the molecular characterization of single CTCs, detecting epithelial (EpCAM, Muc-1, CK5/7), EMT (N-cadherin, Vimentin, Snai1/2, CD117,...
Source: Molecular Oncology - April 19, 2016 Category: Cancer & Oncology Authors: Christina Blassl, Jan Dominik Kuhlmann, Alessandra Webers, Pauline Wimberger, Tanja Fehm, Hans Neubauer Source Type: research
Gene expression profiling of single circulating tumor cells in ovarian cancer – Establishment of a multi-marker gene panel
The presence of circulating tumor cells (CTCs) in the blood of ovarian cancer patients was shown to correlate with decreased overall survival, whereby CTCs with epithelial–mesenchymal-transition (EMT) or stem-like traits are supposed to be involved in metastatic progression and recurrence. Thus, investigating the transcriptional profiles of CTCs might help to identify therapy resistant tumor cells and to overcome treatment failure. For this purpose, we established a multi-marker panel for the molecular characterization of single CTCs, detecting epithelial (EpCAM, Muc-1, CK5/7), EMT (N-cadherin, Vimentin, Snai1/2, CD117, ...
Source: Molecular Oncology - April 19, 2016 Category: Cancer & Oncology Authors: Christina Blassl, Jan Dominik Kuhlmann, Alessandra Webers, Pauline Wimberger, Tanja Fehm, Hans Neubauer Source Type: research
Gene expression profiling of single circulating tumor cells in ovarian cancer - Establishment of a multi-marker gene panel
The presence of circulating tumor cells (CTCs) in the blood of ovarian cancer patients was shown to correlate with decreased overall survival, whereby CTCs with epithelial-mesenchymal-transition (EMT) or stem-like traits are supposed to be involved in metastatic progression and recurrence. Thus, investigating the transcriptional profiles of CTCs might help to identify therapy resistant tumor cells and to overcome treatment failure. For this purpose, we established a multi-marker panel for the molecular characterization of single CTCs, detecting epithelial (EpCAM, Muc-1, CK5/7), EMT (N-cadherin, Vimentin, Snai1/2, CD117, CD...
Source: Molecular Oncology - April 19, 2016 Category: Cancer & Oncology Authors: Christina Blassl, Jan Dominik Kuhlmann, Alessandra Webers, Pauline Wimberger, Hans Neubauer, Tanja Fehm Source Type: research
Mutant p53 proteins counteract autophagic mechanism sensitizing cancer cells to mTOR inhibition
Mutations in TP53 gene play a pivotal role in tumorigenesis and cancer development. Here, we report that gain-of-function mutant p53 proteins inhibit the autophagic pathway favoring antiapoptotic effects as well as proliferation of pancreas and breast cancer cells. We found that mutant p53 significantly counteracts the formation of autophagic vesicles and their fusion with lysosomes throughout the repression of some key autophagy-related proteins and enzymes as BECN1 (and P-BECN1), DRAM1, ATG12, SESN1/2 and P-AMPK with the concomitant stimulation of mTOR signaling. (Source: Molecular Oncology)
Source: Molecular Oncology - April 11, 2016 Category: Cancer & Oncology Authors: Marco Cordani, Elisa Oppici, Ilaria Dando, Elena Butturini, Elisa Dalla Pozza, Mercedes Nadal-Serrano, Jordi Oliver, Pilar Roca, Sofia Mariotto, Barbara Cellini, Giovanni Blandino, Marta Palmieri, Silvia Di Agostino, Massimo Donadelli Source Type: research
KLK6-regulated miRNA networks activate oncogenic pathways in breast cancer subtypes
KLK6 is expressed in normal mammary tissues and is aberrantly regulated in breast cancer. At physiological levels of expression, i.e. those found in normal mammary tissues, KLK6 acts as a tumor suppressor in human breast cancer. However, aberrant overexpression of KLK6 (i.e. 50 –100-fold higher than normal), a characteristic of a subset of human breast cancers is associated with increased tumorigenicity (Pampalakis et al. Cancer Res 69:3779–3787, 2009). Here, we stably transfected KLK6-non-expressing MDA-MB-231 breast cancer cells with the full-length KLK6 cDNA to ove rexpress KLK6 at levels comparable to those observ...
Source: Molecular Oncology - April 7, 2016 Category: Cancer & Oncology Authors: Konstantinos G. Sidiropoulos, Qiang Ding, Georgios Pampalakis, Nicole M.A. White, Peter Boulos, Georgia Sotiropoulou, George M. Yousef Source Type: research
KLK6-regulated miRNA networks activate oncogenic pathways in breast cancer subtypes
KLK6 is expressed in normal mammary tissues and is aberrantly regulated in breast cancer. At physiological levels of expression, i.e. those found in normal mammary tissues, KLK6 acts as a tumor suppressor in human breast cancer. However, aberrant overexpression of KLK6 (i.e. 50 to 100-fold higher than normal), a characteristic of a subset of human breast cancers is associated with increased tumorigenicity (Pampalakis et al. Cancer Res 69:3779-3787, 2009). Here, we stably transfected KLK6-non-expressing MDA-MB-231 breast cancer cells with the full-length KLK6 cDNA to overexpress KLK6 at levels comparable to those observed i...
Source: Molecular Oncology - April 7, 2016 Category: Cancer & Oncology Authors: Konstantinos G. Sidiropoulos, Qiang Ding, Georgios Pampalakis, Nicole M.A. White, Peter Boulos, Georgia Sotiropoulou, George M. Yousef Source Type: research
Towards a new standardized method for circulating miRNAs profiling in clinical studies: Interest of the exogenous normalization to improve miRNA signature accuracy
Circulating miRNAs are promising biomarkers in oncology but have not yet been implemented in the clinic given the lack of concordance across studies. In order to increase the cross-studies reliability, we attempted to reduce and to control the circulating miRNA expression variability between patients. First, to maximize profiling signals and to reduce miRNA expression variability, three isolation kits were compared and the NucleoSpin ® kit provided higher miRNA concentrations than the other widely used kits. (Source: Molecular Oncology)
Source: Molecular Oncology - April 1, 2016 Category: Cancer & Oncology Authors: Nicolas Vigneron, Matthieu Meryet-Figui ère, Audrey Guttin, Jean-Paul Issartel, Bernard Lambert, Mélanie Briand, Marie-Hélène Louis, Mégane Vernon, Pierre Lebailly, Yannick Lecluse, Florence Joly, Sophie Krieger, Stéphanie Lheureux, Bénédicte Clar Source Type: research
Towards a new standardized method for circulating miRNAs profiling in clinical studies: interest of the exogenous normalization to improve miRNA signature accuracy
Circulating miRNAs are promising biomarkers in oncology but have not yet been implemented in the clinic given the lack of concordance across studies. In order to increase the cross-studies reliability, we attempted to reduce and to control the circulating miRNA expression variability between patients. First, to maximize profiling signals and to reduce miRNA expression variability, three isolation kits were compared and the NucleoSpin® kit provided higher miRNA concentrations than the other widely used kits. (Source: Molecular Oncology)
Source: Molecular Oncology - April 1, 2016 Category: Cancer & Oncology Authors: Nicolas Vigneron, Matthieu Meryet-Figuière, Audrey Guttin, Jean-Paul Issartel, Bernard Lambert, Mélanie Briand, Marie-Hélène Louis, Mégane Vernon, Pierre Lebailly, Yannick Lecluse, Florence Joly, Sophie Krieger, Stéphanie Lheureux, Bénédicte Clari Source Type: research
Improving fascin inhibitors to block tumor cell migration and metastasis
Tumor metastasis is the major cause of mortality of cancer patients, being responsible for ∼90% of all cancer deaths. One of the key steps during tumor metastasis is tumor cell migration which requires actin cytoskeletal reorganization. Among the critical actin cytoskeletal protrusion structures are antenna-like filopodia. Fascin protein is the main actin-bundling protein in filopodia. Here we report the development of fascin-specific small-molecules that inhibit the interaction between fascin and actin. (Source: Molecular Oncology)
Source: Molecular Oncology - March 31, 2016 Category: Cancer & Oncology Authors: Shaoqin Han, Jianyun Huang, Bingqian Liu, Bowen Xing, Francois Bordeleau, Cynthia A. Reinhart-King, Wenxin Li, J. Jillian Zhang, Xin-Yun Huang Source Type: research
Improving fascin inhibitors to block tumor cell migration and metastasis
Tumor metastasis is the major cause of mortality of cancer patients, being responsible for ∼90% of all cancer deaths. One of the key steps during tumor metastasis is tumor cell migration which requires actin cytoskeletal reorganization. Among the critical actin cytoskeletal protrusion structures are antenna-like filopodia. Fascin protein is the main actin-bundling protein in filopodia. Here we report the development of fascin-specific small-molecules that inhibit the interaction between fascin and actin. (Source: Molecular Oncology)
Source: Molecular Oncology - March 31, 2016 Category: Cancer & Oncology Authors: Shaoqin Han, Jianyun Huang, Bingqian Liu, Bowen Xing, Francois Bordeleau, Cynthia A. Reinhart-King, Wenxin Li, J. Jillian Zhang, Xin-Yun Huang Source Type: research
Editorial Board
(Source: Molecular Oncology)
Source: Molecular Oncology - March 31, 2016 Category: Cancer & Oncology Source Type: research
