Towards a new standardized method for circulating miRNAs profiling in clinical studies: Interest of the exogenous normalization to improve miRNA signature accuracy

Circulating miRNAs are promising biomarkers in oncology but have not yet been implemented in the clinic given the lack of concordance across studies. In order to increase the cross-studies reliability, we attempted to reduce and to control the circulating miRNA expression variability between patients. First, to maximize profiling signals and to reduce miRNA expression variability, three isolation kits were compared and the NucleoSpin ® kit provided higher miRNA concentrations than the other widely used kits.

Source: Molecular Oncology - April 1, 2016 Category: Cancer & Oncology Authors: Nicolas Vigneron, Matthieu Meryet-Figui ère, Audrey Guttin, Jean-Paul Issartel, Bernard Lambert, Mélanie Briand, Marie-Hélène Louis, Mégane Vernon, Pierre Lebailly, Yannick Lecluse, Florence Joly, Sophie Krieger, Stéphanie Lheureux, Bénédicte Clar Source Type: research