KLK6-regulated miRNA networks activate oncogenic pathways in breast cancer subtypes

KLK6 is expressed in normal mammary tissues and is aberrantly regulated in breast cancer. At physiological levels of expression, i.e. those found in normal mammary tissues, KLK6 acts as a tumor suppressor in human breast cancer. However, aberrant overexpression of KLK6 (i.e. 50 to 100-fold higher than normal), a characteristic of a subset of human breast cancers is associated with increased tumorigenicity (Pampalakis et al. Cancer Res 69:3779-3787, 2009). Here, we stably transfected KLK6-non-expressing MDA-MB-231 breast cancer cells with the full-length KLK6 cDNA to overexpress KLK6 at levels comparable to those observed in patients, and investigated potential oncogenic miRNA networks regulated by these abnormally high KLK6 expression levels and increased activity of this serine protease.

Source: Molecular Oncology - April 7, 2016 Category: Cancer & Oncology Authors: Konstantinos G. Sidiropoulos, Qiang Ding, Georgios Pampalakis, Nicole M.A. White, Peter Boulos, Georgia Sotiropoulou, George M. Yousef Source Type: research