Gene expression profiling of single circulating tumor cells in ovarian cancer – Establishment of a multi-marker gene panel
The presence of circulating tumor cells (CTCs) in the blood of ovarian cancer patients was shown to correlate with decreased overall survival, whereby CTCs with epithelial –mesenchymal-transition (EMT) or stem-like traits are supposed to be involved in metastatic progression and recurrence. Thus, investigating the transcriptional profiles of CTCs might help to identify therapy resistant tumor cells and to overcome treatment failure. For this purpose, we established a multi-marker panel for the molecular characterization of single CTCs, detecting epithelial (EpCAM, Muc-1, CK5/7), EMT (N-cadherin, Vimentin, Snai1/2, CD117, CD146, CD49f) and stem cell (CD44, ALDH1A1, Nanog, SOX2, Notch1/4, Oct4, Lin28) associated transcripts.
Source: Molecular Oncology - Category: Cancer & Oncology Authors: Christina Blassl, Jan Dominik Kuhlmann, Alessandra Webers, Pauline Wimberger, Tanja Fehm, Hans Neubauer Source Type: research
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