Response of distant regions affected by diaschisis commissuralis in one of the most common models of transient focal ischemia in rats
In conclusion, transient focal ischemia affects remote regions of the brain and initiates processes involved in neuronal degeneration in an order which corresponds to the tissue sensitivity to ischemia, namely earlier in the contra-penumbra, and afterwards in the contra-core. The mechanism of secondary damage would influence the progressive neuronal loss of more distant brain regions. (Source: Journal of Chemical Neuroanatomy)
Source: Journal of Chemical Neuroanatomy - August 13, 2019 Category: Neuroscience Source Type: research
Monosynaptic tracing: a step-by-step protocol
Publication date: Available online 10 August 2019Source: Journal of Chemical NeuroanatomyAuthor(s): Thomas K. Lavin, Lei Jin, Ian R. WickershamAbstractMonosynaptic tracing using deletion-mutant rabies virus allows whole-brain mapping of neurons that are directly presynaptic to a targeted population of neurons. The most common and robust way of implementing it is to use Cre mouse lines in combination with Cre-dependent adeno-associated viral vectors for expression of the required genes in the targeted neurons before subsequent injection of the rabies virus. Here we present a step-by-step protocol for performing such experim...
Source: Journal of Chemical Neuroanatomy - August 12, 2019 Category: Neuroscience Source Type: research
Phenotyping neurons activated in the mouse brain during restoration of salt debt
Publication date: Available online 6 August 2019Source: Journal of Chemical NeuroanatomyAuthor(s): Craig M Smith, Joshua Cross, Izel M Eraslan, Aparna Attawar, Sarah Ch’ng, Poshmaal Dhar, Rasika Samarasinghe, Laura Gray, Andrew J LawrenceAbstractSalt overconsumption contributes to hypertension, which is a major risk factor for stroke, heart and kidney disease. Characterising neuronal pathways that may control salt consumption is therefore important for developing novel approaches for reducing salt overconsumption. Here, we identify neurons within the mouse central amygdala (CeA), lateral parabrachial nucleus (LPBN), inte...
Source: Journal of Chemical Neuroanatomy - August 7, 2019 Category: Neuroscience Source Type: research
Spatial and temporal immunoreactivity in the rat brain using an affinity purified polyclonal antibody to DNSP-11
Publication date: Available online 5 August 2019Source: Journal of Chemical NeuroanatomyAuthor(s): James W.H. Sonne, Jason S. Groshong, Corey Seavey, Don M. GashAbstractDNSP-11 antibody signal was investigated in perfusion fixated Fischer 344 rat brains by immunohistochemistry with a custom, affinity purified polyclonal antibody. The DNSP-11-antibody signal was differentially localized from the mature GDNF protein both spatially and temporally. In the mesencephalon of post-natal day 10 animals, when GDNF is maximally expressed, DNSP-11 and GDNF antibody immunoreactivities co-localize extensively but not exclusively. In adu...
Source: Journal of Chemical Neuroanatomy - August 6, 2019 Category: Neuroscience Source Type: research
Pro-apoptotic cell signaling in the prefrontal cortex contributes to depressive-/anxiogenic-like behavioral phenotype of mice subchronically exposed to dexamethasone
Publication date: Available online 30 July 2019Source: Journal of Chemical NeuroanatomyAuthor(s): Suélen Osório Heck, Vanessa Angonesi Zborowski, Simone Pinton, Cristina Wayne NogueiraAbstractAnimal models have been used to investigate the depressive-/anxiogenic-like phenotype induced by synthetic glucocorticoids (GCs). The aim of the present study was to evaluate if apoptosis in the mouse prefrontal cortex (PFC) contributes to the depressive-/anxiogenic-like behavioral phenotype induced by dexamethasone (DEX) subchronic exposure. In the present study, adult male Swiss mice were exposed to DEX (2 mg/kg), or to saline s...
Source: Journal of Chemical Neuroanatomy - July 31, 2019 Category: Neuroscience Source Type: research
Evaluation of retrograde labeling profiles of HSV1 H129 anterograde tracer
Publication date: Available online 23 July 2019Source: Journal of Chemical NeuroanatomyAuthor(s): Peng Su, Huadong Wang, Jinjin Xia, Xin Zhong, Liang Hu, Yingli Li, Yanqiu Li, Min Ying, Fuqiang XuAbstractHerpes simplex virus type 1 H129 strain has been widely used as a useful anterograde neuronal circuit tracing tool. However, whether H129 is a rigorous anterograde tracer and undergoes anterograde-only spreading are questions of significant interest. In the present study, we evaluated the retrograde labeling efficiency of H129 using a TK and ICP34.5 dual deleted H129 recombinant (named as H306) which was replication-defici...
Source: Journal of Chemical Neuroanatomy - July 24, 2019 Category: Neuroscience Source Type: research
Editorial Board
Publication date: September 2019Source: Journal of Chemical Neuroanatomy, Volume 99Author(s): (Source: Journal of Chemical Neuroanatomy)
Source: Journal of Chemical Neuroanatomy - July 6, 2019 Category: Neuroscience Source Type: research
Publisher’s Note: Introducing article numbering to Journal of Chemical Neuroanatomy
Publication date: Available online 2 July 2019Source: Journal of Chemical NeuroanatomyAuthor(s): (Source: Journal of Chemical Neuroanatomy)
Source: Journal of Chemical Neuroanatomy - July 3, 2019 Category: Neuroscience Source Type: research
Nꭃ-nitro-L-arginine methyl model of pre-eclampsia elicits differential IBA1 and EAAT1 expressions in brain
Publication date: Available online 25 June 2019Source: Journal of Chemical NeuroanatomyAuthor(s): Olayemi Kafilat Ijomone, Philemon Shallie, Thajasvarie NaickerAbstractPre-eclampsia (PE) is a pregnancy syndrome associated with an increased risk of both the mother and the baby developing cardiovascular disorders later in life. It is widely accepted that women with severe PE develop a neurological impairment however studies have revealed that the mother and baby are at jeopardy for a neurological deficit later in life. The present study examined expression of Ionized calcium binding adaptor molecule 1 (IBA1) and Excitatory a...
Source: Journal of Chemical Neuroanatomy - June 26, 2019 Category: Neuroscience Source Type: research
Upregulation of hippocampal synaptophysin, GFAP and mGluR3 in a Pilocarpine rat model of epilepsy with history of prolonged febrile seizure
Publication date: Available online 24 June 2019Source: Journal of Chemical NeuroanatomyAuthor(s): Oluwole Ojo Alese, Musa V. MabandlaAbstractDespite the increasing interest and efforts to prevent, treat, and control epilepsy and its clinical manifestation (seizure), the prevalence has not decreased due to incomplete understanding of its etiology. The present study aimed at determining the effect of prolonged febrile seizure (PFS) on the onset of seizure in pilocarpine rat model of temporal lobe epilepsy (TLE).After induction of PFS at postnatal day (PND) 14 in ten out of thirty pups, epilepsy was induced with 350 mg/kg o...
Source: Journal of Chemical Neuroanatomy - June 26, 2019 Category: Neuroscience Source Type: research
Investigation of the neuroprotective effects of thymoquinone on rat spinal cord exposed to 900 MHz electromagnetic field
Publication date: Available online 19 June 2019Source: Journal of Chemical NeuroanatomyAuthor(s): Ahmad yahyazadeh, Berrin Zuhal AltunkaynakAbstractExposure to electromagnetic field in long-term use of cell phones has increased concerns about serious health problems. Our aim was to survey the possible effects of electromagnetic field radiation (60 min/day for 28 days) on the spinal cords of 12 weeks old rats. Further, we investigated whether the administration of thymoquinone (10 mg/kg/day) would protect the spinal cord tissue against the adverse effects of electromagnetic field or not. Twenty-four adult male Wistar al...
Source: Journal of Chemical Neuroanatomy - June 20, 2019 Category: Neuroscience Source Type: research
Neuroprotective effect of exogenous melatonin on the noradrenergic neurons of adult male rats’ locus coeruleus nucleus following REM sleep deprivation
Publication date: Available online 17 June 2019Source: Journal of Chemical NeuroanatomyAuthor(s): Seyed Behnamedin Jameie, Somaye Mesgar, Abbas Aliaghaei, Amir Raoofi, Maryam Amini, Fariba Khodagholi, Samira Danyali, Marziyeh Sorraya, Yousef SadeghiAbstractMelatonin is primarily secreted by the pineal gland in dark. In addition to its role as an internal sleep facilitator, melatonin acts as an antioxidant, anti-inflammatory and neuroprotective agents. melatonin has been introduced as a therapeutic strategy for sleep disorders. Hence, in the present study, we studied the neuroprotective effects of pre- and post-treatment of...
Source: Journal of Chemical Neuroanatomy - June 18, 2019 Category: Neuroscience Source Type: research
A single injection of imipramine affected proliferation in the hippocampus of adult Swiss mice depending on the route of administration, doses, survival time and lodging conditions
Publication date: Available online 13 June 2019Source: Journal of Chemical NeuroanatomyAuthor(s): Martina Podolan, Juliano dos Santos, Thais Walber, Fernanda Possamai, Giordano Gubert Viola, Cilene Lino de OliveiraAbstractSwiss mice may be valuable for the screening of antidepressants in preclinical trials. Acute treatment with antidepressants may affect the behaviour of Swiss mice, but the effects on their hippocampal neurogenesis remain unknown. The present work aims to assess the influence of acute treatment with antidepressants on cell proliferation in the dentate gyrus of the hippocampus of adult Swiss mice. Cell prol...
Source: Journal of Chemical Neuroanatomy - June 14, 2019 Category: Neuroscience Source Type: research
Method for Labeling and Reconstruction of Single Neurons Using Sindbis Virus Vectors
We describe in detail a protocol for single-neuron labeling using Sindbis virus vectors, and we provide an example of a recent project at our laboratory in which we successfully applied these methods to study thalamocortical projection neurons. Further, we discuss the strengths and limitations of Sindbis virus vectors for single neuron reconstruction, comparing them with adeno-associated virus vectors. (Source: Journal of Chemical Neuroanatomy)
Source: Journal of Chemical Neuroanatomy - June 8, 2019 Category: Neuroscience Source Type: research
Curcumin prevents the midbrain dopaminergic innervations and locomotor performance deficiencies resulting from chronic Aluminum exposure in rat
Publication date: Available online 4 June 2019Source: Journal of Chemical NeuroanatomyAuthor(s): Wafaa Laabbar, Abdeljalil Elgot, Omar Elhiba, Halima GamraniAbstractAluminum (Al) among the abundant metals on the earth crust, is able to cross the biological barriers via the gastrointestinal and lung tissues. Once in the body, this heavy metal accumulates in different organs, especially the central nervous system. Though its influence is evidently shown in the substantia nigra of Parkinson’s disease patients and other brain areas in other neurodegenerative diseases, few studies have demonstrated that Al could trigger profo...
Source: Journal of Chemical Neuroanatomy - June 4, 2019 Category: Neuroscience Source Type: research
