Pulsed electromagnetic fields regulate osteocyte apoptosis, RANKL/OPG expression, and its control of osteoclastogenesis depending on the presence of primary cilia
This study enriches our basic knowledge for further understanding the biological behaviors of osteocytes, and is also helpful for providing a more comprehensive mechanistic understanding of the effect of electromagnetic stimulation on bone and relevant skeletal diseases (e.g., bone fractu re and osteoporosis). AbstractGrowing evidence has shown that pulsed electromagnetic fields (PEMF) can modulate bone metabolism in vivo and regulate the activities of osteoblasts and osteoclasts in vitro. Osteocytes, accounting for 95% of bone cells, act as the major mechanosensors in bone for transducing external mechanical signals and p...
Source: Journal of Cellular Physiology - November 13, 2018 Category: Cytology Authors: Pan Wang,
Chi Tang,
Junjie Wu,
Yuefan Yang,
Zedong Yan,
Xiyu Liu,
Xi Shao,
Mingming Zhai,
Jie Gao,
Shengru Liang,
Erping Luo,
Da Jing Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
Key microRNAs in the biology of breast cancer; emerging evidence in the last decade
A large number of microRNAs (miRNAs) have been demonstrated to be implicated in breast cancer (BC) biology such as cell proliferation, migration, invasion, and metastasis by various molecular pathways and targeting that result in up ‐ or downregulation of particular involved genes. AbstractmicroRNAs (miRNAs) are a family of small noncoding RNAs that play a pivotal role in the regulation of main biological and physiological processes, including cell cycle regulation, proliferation, differentiation, apoptosis, stem cell maintenance, and organ development. Dysregulation of these tiny molecules has been related to different ...
Source: Journal of Cellular Physiology - November 13, 2018 Category: Cytology Authors: Monireh Khordadmehr,
Roya Shahbazi,
Hamed Ezzati,
Farinaz Jigari ‐Asl,
Sanam Sadreddini,
Behzad Baradaran Tags: REVIEW ARTICLE Source Type: research
Identification and characterization of circular RNAs involved in mechanical force ‐induced periodontal ligament stem cells
Circular RNAs (circRNAs) are a large class of animal RNAs with regulatory potency. Mechanical force (MF) can induce the expression changes of circRNAs in periodontal ligament stem cells (PDLSCs) which may modulate the orthodontic tooth movement process and the alveolar bone remodeling. CircRNAs may serve as miRNAs sponges by regulating one or several miRNA/miRNAs to affect the osteogenic differentiation in MF ‐induced PDLSCs AbstractCircular RNAs (circRNAs) play critical roles in signal transduction during cell proliferation, differentiation, and apoptosis in a posttranscriptional manner. Recently, circRNAs have been pro...
Source: Journal of Cellular Physiology - November 13, 2018 Category: Cytology Authors: Hong Wang,
Cheng Feng,
Ye Jin,
Wanye Tan,
Fulan Wei Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
Let ‐7g‐5p regulates mouse mammary cells differentiation and function by targeting PRKCA
In this study, to unravel the role of let‐7g‐5p, we found PRKCA (PKC‐alpha, PKC‐α) might serve as potential targets of let ‐7g‐5p by bioinformatics. Then let‐7g‐5p was knocked down by an antisense oligonucleotide in mouse primary mammary epithelial cells. As predicted,PRKCA gene expression and mammary epithelial cells growth were increased by anti ‐let‐7g‐5p regulation in vitro. By using reporter constructs, it showed that the let‐7g‐5p could direct binding with 3′‐the untranslated regions (3′‐UTR) of PRKCA mRNA. Furthermore, ectopic overexpression of let‐7g‐5p in vivo reduced PRKCA a...
Source: Journal of Cellular Physiology - November 13, 2018 Category: Cytology Authors: Lei Tian,
Ye Li,
Chunmei Wang,
Qingzhang Li Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
miR ‐150 might inhibit cell proliferation and promote cell apoptosis by targeting LMO4 in Burkitt lymphoma
This study aimed at investigating the effect of microRNA ‐150 (miR‐150) on cell proliferation of Burkitt lymphoma and its molecular mechanism. Gene expression analysis was applied to identify target genes of miR‐150 in Burkitt lymphoma cell line ST486 based on the dataset from the Gene Expression Omnibus (GEO) datasets GSE86432. miRNA mimics, inhibi tor and small interfering RNA (siRNA) were fluorescently labeled by Cy3, whereas plasmid vector was labeled by EGFP. Cells were viewed by fluorescence microscope and transfection efficiency was evaluated through fluorescent cell percentage. Quantitative real‐time polym...
Source: Journal of Cellular Physiology - November 13, 2018 Category: Cytology Authors: Dandan Zhang,
Yanshuan Wei,
Jun Zhou,
Guannan Wang,
Lin Xiao,
Jingjing Xu,
Na Wei,
Wencai Li,
Mingzhi Zhang Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
miR ‐483 inhibits bovine myoblast cell proliferation and differentiation via IGF1/PI3K/AKT signal pathway
MicroRNA ‐483 (miR‐483) inhibits bovine myoblast cell proliferation and differentiation by targeting insulin‐like growth factor 1 (IGF1) and downregulated the expression of key proteins in the PI3K/AKT signaling pathway. These results will provide an insight target in breeding strategies appealing to c attle industry. AbstractMicroRNAs (miRNAs) have been established to regulate skeletal muscle development in mammals. However, few studies have been conducted on the regulation of proliferation and differentiation of bovine myoblast cells by miRNAs. The aim of our study was to explore the function of miR ‐483 in cell ...
Source: Journal of Cellular Physiology - November 13, 2018 Category: Cytology Authors: Chengchuang Song,
Zhaoxin Yang,
Dong Dong,
Jiawei Xu,
Jian Wang,
Hui Li,
Yongzhen Huang,
Xianyong Lan,
Chuzhao Lei,
Yun Ma,
Hong Chen Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
Potentiation of paclitaxel effect by resveratrol in human breast cancer cells by counteracting the 17 β‐estradiol/estrogen receptor α/neuroglobin pathway
Res treatment does not reduce cancer cell viability by itself Res interferes with estradiol/estrogen receptor α‐induced NGB upregulation in breast cancer cells This new Res‐based mechanism potentiate cancer cell susceptibility to chemotherapeutic agent AbstractNeuroglobin (NGB), an antiapoptotic protein upregulated by 17 β‐estradiol (E2), is part of E2/estrogen receptor α (ERα) pathway pointed to preserve cancer cell survival in presence of microenvironmental stressors including chemotherapeutic drugs. Here, the possibility that resveratrol (Res), an anticancer plant polyphenol, could increase the susceptibilit y...
Source: Journal of Cellular Physiology - November 13, 2018 Category: Cytology Authors: Manuela Cipolletti,
Emiliano Montalesi,
Maria Teresa Nuzzo,
Marco Fiocchetti,
Paolo Ascenzi,
Maria Marino Tags: RAPID COMMUNICATION Source Type: research
Serenoa repens and N ‐acetyl glucosamine/milk proteins complex differentially affect the paracrine communication between endothelial and follicle dermal papilla cells
1)Paracrine communication between endothelial and follicle dermal papilla cells.
2)Characterization of the role of natural compounds on in vutro cocultures systems.
3)mechanistic background for the potential use of natural compounds as promoters of HF vascularization. AbstractCurrent treatments for hair follicle (HF) disruption are based on 5 ‐α reductase inhibitors and prostaglandin modulators. Botanicals and nutraceutical compounds interfere with hair loss or stimulate its partial regrowth. Here, we used in vitro cocultures to investigate the activity ofSerenoa repens (SR) andN‐acetyl glucosamine + milk proteins...
Source: Journal of Cellular Physiology - November 12, 2018 Category: Cytology Authors: Eleonora Bassino,
Franco Gasparri,
Luca Munaron Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
Medical Cannabis: A plurimillennial history of an evergreen
We report through the centuries evidence of its use in numerous pathologic conditions especially for its anti ‐inflammatory, antiseptic, and anticonvulsing properties that support the requirement to direct our present research efforts into the definitive understanding of its efficacy. (Source: Journal of Cellular Physiology)
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: S. Pisanti,
M. Bifulco Tags: REVIEW ARTICLE Source Type: research
Activation of Pyk2 by CaM kinase II in cultured hypothalamic neurons and gonadotroph cells
We reported previously that the δ2 isoform of Ca2+/calmodulin ‐dependent protein kinase II (CaM kinase IIδ2) was involved in GnRH‐induced ERK activation in cultured GnRH neurons (GT1–7 cells). Recently, we found that GnRH treatment of GT1–7 cells activated proline‐rich tyrosine kinase 2 (Pyk2), and Pyk2 was involved in ERK activation. In the current study, we examined the possibility that CaM kinase IIδ2 might activate Pyk2. Knockdown of CaM kinase IIδ2 and KN93, an inhibitor of CaM kinases, inhibited the GnRH‐induced activation of Pyk2. In the case of cultured gonadotroph cells (αT3‐1 cells), knockdown ...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Shiho Okitsu ‐Sakurayama,
Sayomi Higa‐Nakamine,
Hidetsugu Torihara,
Hana Takahashi,
Shigeki Higashiyama,
Hideyuki Yamamoto Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
Impaired DNA double ‐strand breaks repair by kinesin family member 4A inhibition renders human H1299 non‐small‐cell lung cancer cells sensitive to cisplatin
Cisplatin treatment stimulates the expression of kinesin family member 4A (KIF4A) protein in human non ‐small‐cell lung cancer (NSCLC) cells. KIF4A knockdown suppresses the formation of BRCA2 and Rad51 foci and limits the further increase in poly(ADP‐ribose) polymerase 1 activity, thereby enhancing cisplatin‐induced cell cycle arrest in S and G2/M phases and cytotoxicity in human NSCLC cells. KIF4A functions as a novel modulator of cisplatin sensitivity in human NSCLC cells via multiple mechanisms. AbstractPatients with non ‐small‐cell lung cancer (NSCLC) are routinely treated with the platinum‐based chemothe...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Qing Wan,
Yong Shen,
Huzi Zhao,
Bei Wang,
Lei Zhao,
Yongchen Zhang,
Xiaodong Bu,
Meiling Wan,
Chuanlu Shen Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
Prognostic value of a gene signature in clear cell renal cell carcinoma
In conclusion, this 3‐mRNA classifier proved to be a useful tool for prognostic evaluation and could facilitate personalized management of ccRCC p atients. (Source: Journal of Cellular Physiology)
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Liang Chen,
Yongwen Luo,
Gang Wang,
Kaiyu Qian,
Guofeng Qian,
Chin ‐Lee Wu,
Han C. Dan,
Xinghuan Wang,
Yu Xiao Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
Osteoblast autophagy in glucocorticoid ‐induced osteoporosis
Although the pathogenesis of glucocorticoid ‐induced osteoporosis (GIO) has been studied for decades, over the past ten years the autophagy machinery has been implicated as a novel mechanism. Autophagy in osteoblasts, osteocytes, and osteoclasts plays a critical role in the maintenance of bone homeostasis. Herein, we specifically discuss ho w osteoblast autophagy responds to glucocorticoids and its role in the development of GIO. AbstractAdministration of glucocorticoids is an effective strategy for treating many inflammatory and autoimmune diseases. However, glucocorticoid treatment can have adverse effects on bone, lea...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Lufei Wang,
Bradlee L. Heckmann,
Xianrui Yang,
Hu Long Tags: MINI ‐REVIEW Source Type: research
Inhibition of PI3K/Akt/NF ‐κB signaling with leonurine for ameliorating the progression of osteoarthritis: In vitro and in vivo studies
Inhibition of PI3K/Akt/NF ‐κB signaling with leonurine for ameliorating the progression of osteoarthritis: in vitro and in vivo studies AbstractOsteoarthritis (OA) is characterized as the degeneration and destruction of articular cartilage. In recent decades, leonurine (LN), the main active component in medical and edible dual purpose plant Herba Leonuri, has been shown associated with potent anti ‐inflammatory effects in several diseases. In the current study, we examined the protective effects of LN in the inhibition of OA development as well as its underlying mechanism both in vitro and in vivo experiments. In vitr...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Zhi ‐Chao Hu,
Lan‐Fang Gong,
Xiao‐Bin Li,
Xin Fu,
Jiang‐Wei Xuan,
Zhen‐Hua Feng,
Wen‐Fei Ni Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
Fusion with mesenchymal stem cells differentially affects tumorigenic and metastatic abilities of lung cancer cells
This study will contribute to explaning the origin of lung cancer stem cells and to elucidate the role of cell fusion in cancer metastasis. (Source: Journal of Cellular Physiology)
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Li ‐Na Zhang,
Chen‐Fei Kong,
Dan Zhao,
Xian‐Ling Cong,
Shen‐Sen Wang,
Ling Ma,
Ying‐Hui Huang Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
