Let ‐7g‐5p regulates mouse mammary cells differentiation and function by targeting PRKCA

In this study, to unravel the role of let‐7g‐5p, we found PRKCA (PKC‐alpha, PKC‐α) might serve as potential targets of let ‐7g‐5p by bioinformatics. Then let‐7g‐5p was knocked down by an antisense oligonucleotide in mouse primary mammary epithelial cells. As predicted,PRKCA gene expression and mammary epithelial cells growth were increased by anti ‐let‐7g‐5p regulation in vitro. By using reporter constructs, it showed that the let‐7g‐5p could direct binding with 3′‐the untranslated regions (3′‐UTR) of PRKCA mRNA. Furthermore, ectopic overexpression of let‐7g‐5p in vivo reduced PRKCA and β‐casein protein expression as well as inhibited mammary gland growth. These results suggested that let‐7g‐5p could inhibit the mammary epithelial cells differentiation and β‐casein protein synthesis and expression through suppression of PRKCA on the cycle of mammary cell differentiation and development and that let‐7g‐ 5p may be a novel important regulated target in mammary cells function.
Source: Journal of Cellular Physiology - Category: Cytology Authors: Tags: ORIGINAL RESEARCH ARTICLE Source Type: research