miR ‐150 might inhibit cell proliferation and promote cell apoptosis by targeting LMO4 in Burkitt lymphoma

This study aimed at investigating the effect of microRNA ‐150 (miR‐150) on cell proliferation of Burkitt lymphoma and its molecular mechanism. Gene expression analysis was applied to identify target genes of miR‐150 in Burkitt lymphoma cell line ST486 based on the dataset from the Gene Expression Omnibus (GEO) datasets GSE86432. miRNA mimics, inhibi tor and small interfering RNA (siRNA) were fluorescently labeled by Cy3, whereas plasmid vector was labeled by EGFP. Cells were viewed by fluorescence microscope and transfection efficiency was evaluated through fluorescent cell percentage. Quantitative real‐time polymerase chain reaction analysi s (qRT‐PCR) and western blot were applied to detect the expression level of miR‐150 andLMO4. Cell proliferation, cell cycle, and apoptosis were explored by CCK ‐8, flow cytometry. Targeting relationship was validated by the Luciferase reporter assay. Tumor xenograft and immunohistochemical analysis were conducted in nude mice model. In Burkitt lymphoma cells, miR‐150 expression was significantly lower than normal ones, whereas the expression ofLMO4 was upregulated. miR ‐150 might inhibit cell proliferation and promoted apoptosis in Burkitt lymphoma deterioration by downregulatingLMO4. The results of tumor xenograft further confirmed the role of miR ‐150 in Burkitt lymphoma. TargetingLMO4 is a significant mechanism by which miR ‐150 suppresses cell growth and promotes apoptosis in Burkitt lymphoma cells, thus may pro...
Source: Journal of Cellular Physiology - Category: Cytology Authors: Tags: ORIGINAL RESEARCH ARTICLE Source Type: research