Editorial Board
Publication date: August 2019Source: The Crop Journal, Volume 7, Issue 4Author(s): (Source: The Crop Journal)
Source: The Crop Journal - July 25, 2019 Category: Food Science Source Type: research
Improving the efficiency of the CRISPR-Cas12a system with tRNA-crRNA arrays
Publication date: Available online 22 July 2019Source: The Crop JournalAuthor(s): Xixun Hu, Xiangbing Meng, Jiayang Li, Kejian Wang, Hong YuAbstractCRISPR-Cas12a offers a convenient tool for multiplex genome editing in rice. However, the CRISPR-Cas12a system displays variable editing efficiency across genomic loci, with marked influence by CRISPR RNAs (crRNAs). To improve the efficiency of the CRISPR-Cas12a system for multiplex genome editing, we identified various architectures and expression strategies for crRNAs. Transformation of binary vectors loaded with engineered CRISPR-Cas12a systems into rice calli and subsequent...
Source: The Crop Journal - July 24, 2019 Category: Food Science Source Type: research
A wheat chromosome 5AL region confers seedling resistance to both tan spot and Septoria nodorum blotch in two mapping populations
In this study, two recombinant inbred line populations, ‘Bartai’ × ‘Ciano T79’ (referred to as B × C) and ‘Cascabel’ × ‘Ciano T79’ (C × C) were tested for TS and SNB response in order to determine the genetic basis of seedling resistance. Genotyping was performed with the DArTseq genotyping-by-sequencing (GBS) platform. A chromosome region on 5AL conferred resistance to TS and SNB in both populations, but the effects were larger in B × C (R2 = 11.2%–16.8%) than in C × C (R2 = 2.5%–9.7%). Additionally, the chromosome region on 5BL (presumably Tsn1) was significant fo...
Source: The Crop Journal - July 19, 2019 Category: Food Science Source Type: research
Meta-analysis of QTL for Fusarium head blight resistance in Chinese wheat landraces
In this study, we used maps of single nucleotide polymorphisms (SNP) developed from the five populations and identified 31 QTL on 16 chromosomes; 10 QTL were new. We constructed a consensus map and identified six meta-QTL (MQTL) and SNP within the MQTL regions using meta-analysis. Two of the MQTL were on chromosome 3BS (3BSd and 3BSc), and one on each of chromosomes 3A, 2D, 3D, and 4D. Twenty-two SNP closely linked to MQTL were converted into breeder friendly Kompetitive Allele Specific Polymerase Chain Reaction (KASP) assays, which should be useful for marker-assisted selection in breeding programs. (Source: The Crop Journal)
Source: The Crop Journal - July 19, 2019 Category: Food Science Source Type: research
Breeding wheat for resistance to Fusarium head blight in the Global North: China, USA and Canada
The objective of this paper is to review progress made in wheat breeding for Fusarium head blight (FHB) resistance in China, the United States of America (USA) and Canada. In China, numerous Chinese landraces possessing high levels of FHB resistance were grown before the 1950s. Later, pyramiding multiple sources of FHB resistance from introduced germplasm such as Mentana and Funo and locally adapted cultivars played a key role in combining satisfactory FHB resistance and high yield potential in commercial cultivars. Sumai 3, a Chinese spring wheat cultivar, became a major source of FHB resistance in the USA and Canada, and...
Source: The Crop Journal - July 19, 2019 Category: Food Science Source Type: research
