Identification and characterization of a LuxI/R-type quorum sensing system in Pseudoalteromonas
In this study, genome sequencing of Pseudoalteromonas sp. R3 was performed. Accordingly, a QS working model including three sets of hierarchically organized QS systems was proposed in strain R3. Among them, the typical LuxI/R-type QS system using acyl-homoserine lactones (AHLs) as signal molecules was characterized. Sequence similarity analysis indicated luxI encoding AHLs synthase is novel. The luxR encoding AHLs receptor is directly adjacent to luxI downstream. Notably, mutagenesis demonstrated LuxI and LuxR affect each other at transcriptional level, and both control the AHLs formation. Interestingly, it was found that ...
Source: Research in Microbiology - July 17, 2019 Category: Microbiology Source Type: research
ECF-type ABC transporters for uptake of vitamins and transition metal ions into prokaryotic cells
Publication date: Available online 5 July 2019Source: Research in MicrobiologyAuthor(s): Friedrich Finkenwirth, Thomas EitingerAbstractEnergy-coupling factor (ECF) transporters mediate the uptake of micronutrients in prokaryotes. They consist of two ATP-binding-cassette family ATPases, a transmembrane coupling protein (T component) and a substrate-binding membrane protein (S component). ECF transporters for Co2+ and Ni2+ ions have one or two additional proteins with extracytoplasmic regions but poorly understood function. Homologs of T components with a predicted localization in plastids are widespread in plants but their ...
Source: Research in Microbiology - July 6, 2019 Category: Microbiology Source Type: research
Genetics and proteomics analyses reveal the roles of PhoB1 and PhoB2 regulators in bacterial responses to arsenite and phosphate
Publication date: Available online 3 July 2019Source: Research in MicrobiologyAuthor(s): Zixu Qiao, Jing Huang, Yajing Cao, Kaixiang Shi, Gejiao WangAbstractIn bacteria, phosphate (Pi) stress response is governed by the two-component regulatory system, sensor kinase PhoR and its cognate response regulatory protein PhoB. The arsenite [As(III)]-oxidizing bacterium Agrobacterium tumefaciens GW4 contains two phoB genes, phoB1 and phoB2. phoB1 is adjacent to As(III)-oxidizing genes, however, the functions of PhoB1 and PhoB2 remain unclear. Here, phoB1 and phoB2 were each deleted in-frame, and proteomics, qRT-PCR and protein-DNA...
Source: Research in Microbiology - July 4, 2019 Category: Microbiology Source Type: research
Lactonase SsoPox modulates CRISPR-Cas expression in Gram-negative proteobacteria using AHL-based Quorum Sensing systems
Publication date: Available online 3 July 2019Source: Research in MicrobiologyAuthor(s): Sonia Mion, Laure Plener, Benjamin Rémy, David Daudé, Éric ChabrièreAbstractQuorum sensing (QS) is a molecular communication system that bacteria use to harmonize the regulation of genes in a cell density-dependent manner. In proteobacteria, QS is involved, among others, in virulence, biofilm formation or CRISPR-Cas gene regulation. Here, we report for the first time the effect of a QS-interfering enzyme to alter the regulation of CRISPR-Cas systems in model and clinical strains of Pseudomonas aeruginosa, as well as in the marine b...
Source: Research in Microbiology - July 4, 2019 Category: Microbiology Source Type: research
Comparison of Boreal Acid Sulfate Soil Microbial Communities in Oxidative and Reductive Environments
In this study, five sites were investigated for the occurrence of acid sulfate soils and their geochemistry and microbiology was identified. The pH and soil chemistry identified three of the areas as having classical acid sulfate soil characteristics and culture independent identification of 16S rRNA genes identified populations related to acidophilic bacteria capable of catalyzing sulfidic mineral dissolution, including species likely adapted to low temperature. These results were compared to an acid sulfate soil area that had been flooded for ten years and showed that the previously oxidized sulfidic materials had an inc...
Source: Research in Microbiology - July 4, 2019 Category: Microbiology Source Type: research
Taxonomic assignment of uncultured prokaryotes with long range PCR targeting the spectinomycin operon
Publication date: Available online 3 July 2019Source: Research in MicrobiologyAuthor(s): Jean-Pierre Flandrois, Céline Brochier-Armanet, Jérôme Briolay, Danis Abrouk, Guillaume Schwob, Philippe Normand, Maria P. FernandezAbstractThe taxonomic assignment of uncultured prokaryotes to known taxa is a major challenge in microbial systematics. This relies usually on the phylogenetic analysis of the ribosomal small subunit RNA or a few housekeeping genes. Recent works have disclosed ribosomal proteins as valuable markers for systematics and, due to the boom in complete genome sequencing, their use has become widespread. Yet, ...
Source: Research in Microbiology - July 4, 2019 Category: Microbiology Source Type: research
The CydDC family of transporters
Publication date: Available online 3 July 2019Source: Research in MicrobiologyAuthor(s): Robert K. Poole, Adam G. Cozens, Mark Shepherd (Source: Research in Microbiology)
Source: Research in Microbiology - July 4, 2019 Category: Microbiology Source Type: research
Multidrug ABC transporters in bacteria
Publication date: Available online 25 June 2019Source: Research in MicrobiologyAuthor(s): Cédric Orelle, Khadija Mathieu, Jean-Michel JaultAbstractMultidrug efflux transporters are a plague in the antibiotic resistance mechanisms as they confer the capacity of bacteria to evade most of current therapies. Although these transporters were initially discovered as proton-motive driven pumps, another class of multidrug efflux transporters has emerged in the mid-90s that are powered by ATP hydrolysis. This new class of transporters belongs to one of the largest families of proteins, the ATP-Binding Cassette (ABC) transporters, ...
Source: Research in Microbiology - June 27, 2019 Category: Microbiology Source Type: research
Editorial board
Publication date: April–May 2019Source: Research in Microbiology, Volume 170, Issue 3Author(s): (Source: Research in Microbiology)
Source: Research in Microbiology - June 19, 2019 Category: Microbiology Source Type: research
Obituary: Georges Cohen (1920–2018)
Publication date: Available online 3 June 2019Source: Research in MicrobiologyAuthor(s): Pascale Cossart, Philippe Marlière (Source: Research in Microbiology)
Source: Research in Microbiology - June 5, 2019 Category: Microbiology Source Type: research
Kinetic analysis of denitrification coupled with Cd(II) removal by Cupriavidus sp. CC1 and its removal mechanism
Publication date: Available online 17 May 2019Source: Research in MicrobiologyAuthor(s): Jun feng Su, Lei Xue, Ting lin Huang, Zhao Wang, Jia xing WangAbstractA simultaneous denitrification and Cd (II) removal of strain CC1 was isolated from Li Jiahe oligotrophic reservoir of Xi’an (China). Strain CC1 was identified as Cupriavidus sp. The first-order reaction kinetics equation was to model kinetic processes of mixotrophic anaerobic denitrification and Cd(II) removal, which showed an optimal NO3--N removal rate was obtained at the C/N ratio of 5.0, temperature of 30.0°C and Fe(II) concentration of 15.0 mg·L-1. A highest...
Source: Research in Microbiology - May 17, 2019 Category: Microbiology Source Type: research
Obituary: Agnes Ullmann (1927–2019)
Publication date: Available online 15 May 2019Source: Research in MicrobiologyAuthor(s): Daniel Ladant, Didier Mazel, Maxime Schwartz (Source: Research in Microbiology)
Source: Research in Microbiology - May 16, 2019 Category: Microbiology Source Type: research
Microbial community composition of water samples stored inside the International Space Station
Publication date: Available online 7 May 2019Source: Research in MicrobiologyAuthor(s): Giovanni Bacci, Stefano Amalfitano, Caterina Levantesi, Simona Rossetti, Laurent Garrelly, Francesco Canganella, Giovanna Bianconi, Vincenzo Di Pilato, Gian Maria Rossolini, Alessio Mengoni, Renato Fani, Elena Perrin, The Biowyse ConsortiumAbstractDuring the VIABLE ISS project (eValuatIon And monitoring of microBiofiLms insidE International Space Station), water samples subjected to two different silver treatments were sent and kept on board the International Space Station (ISS) from 2011 to 2016. In this note we report data on the viab...
Source: Research in Microbiology - May 9, 2019 Category: Microbiology Source Type: research
Effect of the optimized selective enrichment medium on the expression of the p60 protein used as Listeria monocytogenes antigen in specific sandwich ELISA
Publication date: Available online 3 April 2019Source: Research in MicrobiologyAuthor(s): Marie-Christine Etty, Sabato D'Auria, Carole Fraschini, Stephane Salmieri, Monique LacroixAbstractThis paper presents the effects of the composition of different media (i.e., Tryptic soy broth (TSB), Brain heart infusion (BHI), Listeria enrichment broth (LEB), Fraser broth (FB) and University of Vermont medium (UVM)) on the detection of a short peptide fragment PepD specific to the p60 protein (p60) of L. monocytogenes by a monoclonal antibody (anti-PepD mAb). Expression of the p60 obtained was demonstrated to be proportional to the ...
Source: Research in Microbiology - April 29, 2019 Category: Microbiology Source Type: research
Comparative genomics and proteogenomics highlight key molecular players involved in Frankia sporulation
Publication date: Available online 21 April 2019Source: Research in MicrobiologyAuthor(s): Lorine Bethencourt, Hasna Boubakri, Najwa Taib, Philippe Normand, Jean Armengaud, Pascale Fournier, Céline Brochier-Armanet, Aude Herrera-BelaroussiAbstractSporulation is a microbial adaptive strategy to resist inhospitable conditions for vegetative growth and to disperse to colonise more favourable environments. This microbial trait is widespread in Actinobacteria. Among them, Frankia strains are able to differentiate sporangia in pure culture, while others can sporulate even when in symbiosis with sporulation occurring within host...
Source: Research in Microbiology - April 21, 2019 Category: Microbiology Source Type: research
