Imaging redox activity and Fe(II) at the microbe-mineral interface during Fe(III) reduction
Publication date: Available online 7 June 2018Source: Research in MicrobiologyAuthor(s): Helen F. Downie, Joel P. Standerwick, Letitia Burgess, Louise S. Natrajan, Jonathan R. LloydAbstractDissimilatory iron-reducing bacteria (DIRB) play an important role in controlling the redox chemistry of Fe and other transition metals and radionuclides in the environment. During bacterial iron reduction, electrons are transferred from the outer membrane to poorly soluble Fe(III) minerals, although the precise physiological mechanisms and local impact on minerals of these redox processes remain unclear. The aim of this work was to use ...
Source: Research in Microbiology - July 5, 2018 Category: Microbiology Source Type: research
High precision microfluidic microencapsulation of bacteriophages for enteric delivery
Publication date: Available online 7 June 2018Source: Research in MicrobiologyAuthor(s): Gurinder K. Vinner, Danish J. MalikAbstractA Salmonella specific bacteriophage Felix O1 (Myoviridae) was microencapsulated in a pH responsive polymer formulation. The formulation incorporated a pH responsive methacrylic acid copolymer Eudragit® S100 (10% (w/v)) with the addition of the biopolymer sodium alginate, the composition of which was varied in the range (0.5% (w/v) - 2% (w/v)). The microencapsulation process employed commercially available microfluidic droplet generation devices. We have used readily available low cost microfl...
Source: Research in Microbiology - July 5, 2018 Category: Microbiology Source Type: research
Peptides as biosorbents – Promising tools for resource recovery
We present an approach to identify peptides for resource recovery using Phage Surface Display. Here, we describe the development of peptides for binding of rare earth element terbium-containing solids and for removal and enrichment of the heavy metal ions of cobalt and nickel out of waste waters and leaching solutions. We identified phage displaying specific peptides with ∼100× enhanced affinity towards terbium-containing solids or ∼20× enhanced affinity towards nickel (∼3× cobalt). (Source: Research in Microbiology)
Source: Research in Microbiology - July 5, 2018 Category: Microbiology Source Type: research
Adaptive response of yeast cells to triggered toxicity of phosphoribulokinase
Publication date: Available online 30 June 2018Source: Research in MicrobiologyAuthor(s): Catherine Rouzeau, Adilya Dagkesamanskaya, Krzysztof Langer, Jérôme Bibette, Jean Baudry, Denis Pompon, Véronique Anton-LeberreAbstractAdjustment of plasmid copy number resulting from the balance between positive and negative impacts of borne synthetic genes, plays a critical role in the global efficiency of multistep metabolic engineering. Differential expression of co-expressed engineered genes is frequently observed depending on growth phases, metabolic status and triggered adjustments of plasmid copy numbers, constituting a dyn...
Source: Research in Microbiology - July 5, 2018 Category: Microbiology Source Type: research
Adaptive response of yeast cells to triggered toxicity of phosphoribulokinase
Publication date: Available online 30 June 2018 Source:Research in Microbiology Author(s): Catherine Rouzeau, Adilya Dagkesamanskaya, Krzysztof Langer, Jérôme Bibette, Jean Baudry, Denis Pompon, Véronique Anton-Leberre Adjustment of plasmid copy number resulting from the balance between positive and negative impacts of borne synthetic genes, plays a critical role in the global efficiency of multistep metabolic engineering. Differential expression of co-expressed engineered genes is frequently observed depending on growth phases, metabolic status and triggered adjustments of plasmid copy numbers, constituting a ...
Source: Research in Microbiology - June 30, 2018 Category: Microbiology Source Type: research
Peptides as Biosorbents – Promising tools for Resource Recovery
We present an approach to identify peptides for resource recovery using Phage Surface Display. Here, we describe the development of peptides for binding of rare earth element terbium-containing solids and for removal and enrichment of the heavy metal ions of cobalt and nickel out of waste waters and leaching solutions. We identified phage displaying specific peptides with ∼100x enhanced affinity towards terbium-containing solids or ∼20x enhanced affinity towards nickel (∼3x cobalt). (Source: Research in Microbiology)
Source: Research in Microbiology - June 19, 2018 Category: Microbiology Source Type: research
Real-time assessment of bacteriophage T3-derived antimicrobial activity against planktonic and biofilm-embedded Escherichia coli by isothermal microcalorimetry
Publication date: Available online 7 June 2018 Source:Research in Microbiology Author(s): Tamta Tkhilaishvili, Mariagrazia Di Luca, Gerardo Abbandonato, Elena Maryka Maiolo, Ann-Brit Klatt, Monika Reuter, Elisabeth Möncke-Buchner, Andrej Trampuz Bacterial biofilms, highly resistant to the conventional antimicrobial therapy, remain an unresolved challenge pressing the medical community to investigate new and alternative strategies to fight chronic implant-associated infections. Recently, strictly lytic bacteriophages have been revalued as powerful agents to kill antibiotic-resistant bacteria even in biofilm. Here...
Source: Research in Microbiology - June 7, 2018 Category: Microbiology Source Type: research
Imaging redox activity and Fe(II) at the microbe-mineral interface during Fe(III) reduction
Publication date: Available online 7 June 2018 Source:Research in Microbiology Author(s): Helen F. Downie, Joel P. Standerwick, Letitia Burgess, Louise S. Natrajan, Jonathan R. Lloyd Dissimilatory iron-reducing bacteria (DIRB) play an important role in controlling the redox chemistry of Fe and other transition metals and radionuclides in the environment. During bacterial iron reduction, electrons are transferred from the outer membrane to poorly soluble Fe(III) minerals, although the precise physiological mechanisms and local impact on minerals of these redox processes remain unclear. The aim of this work was to use...
Source: Research in Microbiology - June 7, 2018 Category: Microbiology Source Type: research
High precision microfluidic microencapsulation of bacteriophages for enteric delivery
Publication date: Available online 7 June 2018 Source:Research in Microbiology Author(s): Gurinder K. Vinner, Danish J. Malik A Salmonella specific bacteriophage Felix O1 (Myoviridae) was microencapsulated in a pH responsive polymer formulation. The formulation incorporated a pH responsive methacrylic acid copolymer Eudragit® S100 (10% (w/v)) with the addition of the biopolymer sodium alginate, the composition of which was varied in the range (0.5% (w/v) - 2% (w/v)). The microencapsulation process employed commercially available microfluidic droplet generation devices. We have used readily available low cost microflui...
Source: Research in Microbiology - June 7, 2018 Category: Microbiology Source Type: research
Iron targeted transcriptome study draws attention to novel redox protein candidates involved in ferrous iron oxidation in “Ferrovum” sp. JA12
Publication date: Available online 2 June 2018 Source:Research in Microbiology Author(s): Sophie R. Ullrich, Anja Poehlein, Gloria Levicán, Martin Mühling, Michael Schlömann The response of the acidophilic iron oxidizer “Ferrovum” sp. JA12 to elevated concentrations of ferrous iron was targeted at transcriptome level in order to assess models on oxidative stress management and ferrous iron oxidation. Overall transcriptome profiles indicate a high cellular activity of “Ferrovum” sp. JA12 up to 50 mM of ferrous iron with genes predicted to be involved in iron oxidation, carbon fixation and ribosome formatio...
Source: Research in Microbiology - June 2, 2018 Category: Microbiology Source Type: research
Changes in the lipid composition of Bradyrhizobium cell envelope reveal a rapid response to water deficit involving lysophosphatidylethanolamine synthesis from phosphatidylethanolamine in outer membrane
Publication date: Available online 2 June 2018 Source:Research in Microbiology Author(s): Adriana B. Cesari, Natalia S. Paulucci, María A. Biasutti, Gustavo M. Morales, Marta S. Dardanelli We evaluate the behavior of the membrane of Bradyrhizobium sp. SEMIA6144 during adaptation to polyethylene glycol (PEG). A dehydrating effect on the morphology of the cell surface, as well as a fluidizing effect on the membrane was observed 10 min after PEG shock; however, the bacteria were able to restore optimal membrane fluidity. Shock for 1 h caused an increase of lysophosphatidylethanolamine in the outer membrane at the expe...
Source: Research in Microbiology - June 2, 2018 Category: Microbiology Source Type: research
C-di-GMP turnover influences motility and biofilm formation in Bacillus amyloliquefaciens PG12
In this study, we characterized the core regulators of c-di-GMP turnover in B. amyloliquefaciens PG12. Using bioinformatic analysis, heterologous expression and biochemical characterization of knockout and overexpression derivatives, we identified and characterized two active diguanylate cyclases (which catalyze c-di-GMP biosynthesis), YhcK and YtrP and one active c-di-GMP phosphodiesterase (which degrades c-di-GMP), YuxH. Furthermore, we showed that elevating c-di-GMP levels up to a certain threshold inhibited the swimming motility of B. amyloliquefaciens PG12. Although yhcK, ytrP and yuxH knockout mutants did not display...
Source: Research in Microbiology - June 1, 2018 Category: Microbiology Source Type: research
The transcription factor SlyA from Salmonella Typhimurium regulates genes in response to hydrogen peroxide and sodium hypochlorite
Publication date: Available online 29 May 2018 Source:Research in Microbiology Author(s): Carolina E. Cabezas, Alan C. Briones, Camila Aguirre, Coral Pardo-Esté, Juan Castro-Severyn, César R. Salinas, María S. Baquedano, Alejando A. Hidalgo, Juan A. Fuentes, Eduardo H. Morales, Claudio A. Meneses, Eduardo Castro-Nallar, Claudia Paz Saavedra Salmonella Typhimurium is an intracellular pathogen that is capable of generating systemic fever in a murine model. Over the course of the infection, Salmonella faces different kinds of stressors, including harmful reactive oxygen species (ROS). Various defence mechani...
Source: Research in Microbiology - May 29, 2018 Category: Microbiology Source Type: research
Syntrophic effect of indigenous and inoculated microorganisms in the leaching of rare earth elements from Western Australian monazite
Publication date: Available online 28 May 2018 Source:Research in Microbiology Author(s): Melissa K. Corbett, Jacques J. Eksteen, Xi-Zhi Niu, Elizabeth LJ. Watkin The unique physiochemical properties exhibited by rare earth elements (REEs) and their increasing application in high-tech industries has created a demand for secure supply lines with established recovery procedures that create minimal environmental damage. Bioleaching experiments conducted on a non-sterile monazite concentrate with a known phosphate solubilising microorganism (PSM) resulted in greater mobilisation of REEs into solution in comparison to exp...
Source: Research in Microbiology - May 28, 2018 Category: Microbiology Source Type: research
Bacteriophage imaging: past, present and future
Publication date: Available online 28 May 2018 Source:Research in Microbiology Author(s): Gabriel MF. Almeida, Miika Leppänen, Ilari J. Maasilta, Lotta-Riina Sundberg The visualization of viral particles only became possible after the advent of the electron microscope. The first bacteriophage images were published in 1940 and were soon followed by many other publications that helped to elucidate the structure of the particles and their interaction with the bacterial hosts. As sample preparation improved and new technologies were developed, phage imaging became important approach to morphologically classify these vir...
Source: Research in Microbiology - May 28, 2018 Category: Microbiology Source Type: research
