Iron targeted transcriptome study draws attention to novel redox protein candidates involved in ferrous iron oxidation in “Ferrovum” sp. JA12
Publication date: Available online 2 June 2018Source: Research in MicrobiologyAuthor(s): Sophie R. Ullrich, Anja Poehlein, Gloria Levicán, Martin Mühling, Michael SchlömannAbstractThe response of the acidophilic iron oxidizer “Ferrovum” sp. JA12 to elevated concentrations of ferrous iron was targeted at transcriptome level in order to assess models on oxidative stress management and ferrous iron oxidation. Overall transcriptome profiles indicate a high cellular activity of “Ferrovum” sp. JA12 up to 50 mM of ferrous iron with genes predicted to be involved in iron oxidation, carbon fixation and ribosome formation...
Source: Research in Microbiology - July 10, 2018 Category: Microbiology Source Type: research
Changes in the lipid composition of Bradyrhizobium cell envelope reveal a rapid response to water deficit involving lysophosphatidylethanolamine synthesis from phosphatidylethanolamine in outer membrane
Publication date: Available online 2 June 2018Source: Research in MicrobiologyAuthor(s): Adriana B. Cesari, Natalia S. Paulucci, María A. Biasutti, Gustavo M. Morales, Marta S. DardanelliAbstractWe evaluate the behavior of the membrane of Bradyrhizobium sp. SEMIA6144 during adaptation to polyethylene glycol (PEG). A dehydrating effect on the morphology of the cell surface, as well as a fluidizing effect on the membrane was observed 10 min after PEG shock; however, the bacteria were able to restore optimal membrane fluidity.Shock for 1 h caused an increase of lysophosphatidylethanolamine in the outer membrane at the expe...
Source: Research in Microbiology - July 10, 2018 Category: Microbiology Source Type: research
Imaging redox activity and Fe(II) at the microbe-mineral interface during Fe(III) reduction
Publication date: Available online 7 June 2018Source: Research in MicrobiologyAuthor(s): Helen F. Downie, Joel P. Standerwick, Letitia Burgess, Louise S. Natrajan, Jonathan R. LloydAbstractDissimilatory iron-reducing bacteria (DIRB) play an important role in controlling the redox chemistry of Fe and other transition metals and radionuclides in the environment. During bacterial iron reduction, electrons are transferred from the outer membrane to poorly soluble Fe(III) minerals, although the precise physiological mechanisms and local impact on minerals of these redox processes remain unclear. The aim of this work was to use ...
Source: Research in Microbiology - July 10, 2018 Category: Microbiology Source Type: research
Real-time assessment of bacteriophage T3-derived antimicrobial activity against planktonic and biofilm-embedded Escherichia coli by isothermal microcalorimetry
Publication date: Available online 7 June 2018Source: Research in MicrobiologyAuthor(s): Tamta Tkhilaishvili, Mariagrazia Di Luca, Gerardo Abbandonato, Elena Maryka Maiolo, Ann-Brit Klatt, Monika Reuter, Elisabeth Möncke-Buchner, Andrej TrampuzAbstractBacterial biofilms, highly resistant to the conventional antimicrobial therapy, remain an unresolved challenge pressing the medical community to investigate new and alternative strategies to fight chronic implant-associated infections. Recently, strictly lytic bacteriophages have been revalued as powerful agents to kill antibiotic-resistant bacteria even in biofilm. Here, th...
Source: Research in Microbiology - July 10, 2018 Category: Microbiology Source Type: research
High precision microfluidic microencapsulation of bacteriophages for enteric delivery
Publication date: Available online 7 June 2018Source: Research in MicrobiologyAuthor(s): Gurinder K. Vinner, Danish J. MalikAbstractA Salmonella specific bacteriophage Felix O1 (Myoviridae) was microencapsulated in a pH responsive polymer formulation. The formulation incorporated a pH responsive methacrylic acid copolymer Eudragit® S100 (10% (w/v)) with the addition of the biopolymer sodium alginate, the composition of which was varied in the range (0.5% (w/v)–2% (w/v)). The microencapsulation process employed commercially available microfluidic droplet generation devices. We have used readily available low cost microfl...
Source: Research in Microbiology - July 10, 2018 Category: Microbiology Source Type: research
Peptides as biosorbents – Promising tools for resource recovery
We present an approach to identify peptides for resource recovery using Phage Surface Display. Here, we describe the development of peptides for binding of rare earth element terbium-containing solids and for removal and enrichment of the heavy metal ions of cobalt and nickel out of waste waters and leaching solutions. We identified phage displaying specific peptides with ∼100× enhanced affinity towards terbium-containing solids or ∼20× enhanced affinity towards nickel (∼3× cobalt). (Source: Research in Microbiology)
Source: Research in Microbiology - July 10, 2018 Category: Microbiology Source Type: research
Adaptive response of yeast cells to triggered toxicity of phosphoribulokinase
Publication date: Available online 30 June 2018Source: Research in MicrobiologyAuthor(s): Catherine Rouzeau, Adilya Dagkesamanskaya, Krzysztof Langer, Jérôme Bibette, Jean Baudry, Denis Pompon, Véronique Anton-LeberreAbstractAdjustment of plasmid copy number resulting from the balance between positive and negative impacts of borne synthetic genes, plays a critical role in the global efficiency of multistep metabolic engineering. Differential expression of co-expressed engineered genes is frequently observed depending on growth phases, metabolic status and triggered adjustments of plasmid copy numbers, constituting a dyn...
Source: Research in Microbiology - July 10, 2018 Category: Microbiology Source Type: research
Temporal evolution of Acinetobacter baumannii ST107 clone: Conversion of blaOXA-143 into blaOXA-231 coupled with mobilization of ISAba1 upstream occAB1
We reported an A. baumannii ST107 clone carrying blaOXA-143 that acquired a mutation resulting into blaOXA-231 and mobilized ISAba1 upstream occAB1. (Source: Research in Microbiology)
Source: Research in Microbiology - July 10, 2018 Category: Microbiology Source Type: research
Characterization of a luxI/luxR-type quorum sensing system and N-acyl homoserine lactone-dependent regulation of exo-enzyme and antibacterial component production in Serratia plymuthica RVH1
Publication date: Available online 14 January 2007Source: Research in MicrobiologyAuthor(s): Rob Van Houdt, Pieter Moons, Abram Aertsen, An Jansen, Kristof Vanoirbeek, Mavis Daykin, Paul Williams, Chris W. MichielsThe Publisher regrets that this article was an accidental duplication of an article that has already been published in Res Microbiol, 158 (2007) 150-158, doi: 10.1016/j.resmic.2006.11.008. The duplicate article has therefore been withdrawn. (Source: Research in Microbiology)
Source: Research in Microbiology - July 5, 2018 Category: Microbiology Source Type: research
Corrigendum to “The microbiome of a striped dolphin (Stenella coeruleoalba) stranded in Portugal” [Res Microbiol (2017) 85–93]
Publication date: Available online 29 January 2017Source: Research in MicrobiologyAuthor(s): Filipa Godoy-Vitorino, Arnold Rodriguez-Hilario, Ana Luísa Alves, Filipa Gonçalves, Beatriz Cabrera-Colon, Cristina Sousa Mesquita, Pedro Soares-Castro, Marisa Ferreira, Ana Marçalo, José Vingada, Catarina Eira, Pedro Miguel Santos (Source: Research in Microbiology)
Source: Research in Microbiology - July 5, 2018 Category: Microbiology Source Type: research
The fascinating but mysterious mechanistic aspects of multidrug transport by MdfA from Escherichia coli
Publication date: Available online 23 September 2017Source: Research in MicrobiologyAuthor(s): Eliane H. Yardeni, Elia Zomot, Eitan BibiAbstractMdfA is an interesting member of a large group of secondary multidrug (Mdr) transporters. Through genetic, biochemical and biophysical studies of MdfA, many challenging aspects of the multidrug transport phenomenon have been addressed. This includes its ability to interact with chemically unrelated drugs and how it utilizes energy to drive efflux of compounds that are not only structurally, but also electrically, different. Admittedly, however, despite all efforts and a recent pion...
Source: Research in Microbiology - July 5, 2018 Category: Microbiology Source Type: research
Clamping down on drugs: the Escherichia coli multidrug efflux protein MdtM
Publication date: Available online 27 September 2017Source: Research in MicrobiologyAuthor(s): Christopher J. Law, Kamela O. AlegreAbstractMultidrug resistance is principally a consequence of the active transport of drugs out of the cell by proteins that are integral membrane transporters. In the following review, we present a synthesis of current understanding of the Escherichia coli multidrug resistance transporter, MdtM, a 410 amino acid residue protein that belongs to the large and ubiquitous major facilitator superfamily (MFS). (Source: Research in Microbiology)
Source: Research in Microbiology - July 5, 2018 Category: Microbiology Source Type: research
Regulation of the AcrAB-TolC efflux pump in Enterobacteriaceae
Publication date: Available online 8 November 2017Source: Research in MicrobiologyAuthor(s): Natasha Weston, Prateek Sharma, Vito Ricci, Laura J.V. PiddockAbstractBacterial multidrug efflux systems are a major mechanism of antimicrobial resistance and are fundamental to the physiology of Gram-negative bacteria. The resistance-nodulation-division (RND) family of efflux pumps is the most clinically significant, as it is associated with multidrug resistance. Expression of efflux systems is subject to multiple levels of regulation, involving local and global transcriptional regulation as well as post-transcriptional and post-t...
Source: Research in Microbiology - July 5, 2018 Category: Microbiology Source Type: research
The hydrophobic trap—the Achilles heel of RND efflux pumps
Publication date: Available online 13 November 2017Source: Research in MicrobiologyAuthor(s): Zachary Aron, Timothy J. OppermanAbstractResistance–nodulation–division (RND) superfamily efflux pumps play a major role in multidrug resistance (MDR) of Gram-negative pathogens by extruding diverse classes of antibiotics from the cell. There has been considerable interest in developing efflux pump inhibitors (EPIs) of RND pumps as adjunctive therapies. The primary challenge in EPI discovery has been the highly hydrophobic, poly-specific substrate binding site of the target. Recent findings have identified the hydrophobic trap...
Source: Research in Microbiology - July 5, 2018 Category: Microbiology Source Type: research
