Lactonase SsoPox modulates CRISPR-Cas expression in Gram-negative proteobacteria using AHL-based Quorum Sensing systems

Publication date: Available online 3 July 2019Source: Research in MicrobiologyAuthor(s): Sonia Mion, Laure Plener, Benjamin Rémy, David Daudé, Éric ChabrièreAbstractQuorum sensing (QS) is a molecular communication system that bacteria use to harmonize the regulation of genes in a cell density-dependent manner. In proteobacteria, QS is involved, among others, in virulence, biofilm formation or CRISPR-Cas gene regulation. Here, we report for the first time the effect of a QS-interfering enzyme to alter the regulation of CRISPR-Cas systems in model and clinical strains of Pseudomonas aeruginosa, as well as in the marine bacterium Chromobacterium violaceum CV12472. The expression of CRISPR-Cas genes decreased in most cases suggesting that enzymatic disruption of QS is promising for modulating phage-bacteria interactions.

Source: Research in Microbiology - July 4, 2019 Category: Microbiology Source Type: research