Transcriptional and hormonal profiling of Fusarium graminearum-infected wheat reveals an association between auxin and susceptibility
Publication date: Available online 19 April 2019Source: Physiological and Molecular Plant PathologyAuthor(s): Elizabeth K. Brauer, Hélène Rocheleau, Margaret Balcerzak, Youlian Pan, François Fauteux, Ziying Liu, Li Wang, Wenju Zheng, Thérèse OuelletAbstractResistance to Fusarium graminearum, a fungal pathogen of cereals, has been associated with increased defense-associated hormonal responses in wheat. A comparison of susceptible (Roblin) and moderately resistant wheat genotypes (Wuhan 1, Nyubai) revealed transcriptional regulation of 1,021 genes during infection. Only 4% of these genes were uniquely differentially ex...
Source: Physiological and Molecular Plant Pathology - April 20, 2019 Category: Molecular Biology Source Type: research

The role of MicroRNAs in defense against viral phytopathogens
Publication date: Available online 17 April 2019Source: Physiological and Molecular Plant PathologyAuthor(s): Behzad Hajieghrari, Naser Farrokhi, Bahram Goliaei, Kaveh KavousiAbstractMicroRNAs have been considered as an endogenous post-transcriptional gene silencing mechanism for transcription regulation. Additionally, reports are indicative of their involvement in plant viral defense. It seems that horizontal miRNA transfer from pathogenic viral and viroid genomes into plants has evolved into the counter-defense mechanism(s) against their invasions. miRNAs of a green alga and 16 other higher plant species, with/without en...
Source: Physiological and Molecular Plant Pathology - April 18, 2019 Category: Molecular Biology Source Type: research

Biological control of Sclerotium fruit rot of snake fruit and stem rot of lettuce by Trichoderma sp. T76-12/2 and the mechanisms involved
Publication date: Available online 10 April 2019Source: Physiological and Molecular Plant PathologyAuthor(s): Prisana Wonglom, Wilailuck Daengsuwan, Shin-ichi Ito, Anurag SunpapaoAbstractFruit rot of snake fruit and stem rot of lettuce caused by Sclerotium sp. SZ01 and LS01, respectively, are major diseases causing devastating crop losses. Tichoderma species has been known as an effective biological control agent (BCA) against several plant disease pathogens. This research aimed to select an effective Trichoderma species for controlling both diseases. Five Trichoderma spp. isolates were subjected to primary screening again...
Source: Physiological and Molecular Plant Pathology - April 10, 2019 Category: Molecular Biology Source Type: research

DNA-based electrochemical nanobiosensor for the detection of Phytophthora palmivora (Butler) Butler, causing black pod rot in cacao (Theobroma cacao L.) pods
Publication date: Available online 9 April 2019Source: Physiological and Molecular Plant PathologyAuthor(s): Anthony James DM. Franco, Florinia E. Merca, Myrna S. Rodriguez, Johnny F. Balidion, Veronica P. Migo, Divina M. Amalin, Evangelyn C. Alocilja, Lilia M. FernandoAbstractA nanobiosensor was developed for the electrochemical detection of Phytophthora palmivora, a notorious pathogen of cacao causing severe crop loss. Sandwich hybrids between two oligonucleotide probes and the genomic DNA of P. palmivora were prepared and electrochemically detected. The oligonucleotide probes were designed based on the ITS sequence of t...
Source: Physiological and Molecular Plant Pathology - April 9, 2019 Category: Molecular Biology Source Type: research

Quorum sensing interruption as a tool to control virulence of plant pathogenic bacteria
Publication date: Available online 9 April 2019Source: Physiological and Molecular Plant PathologyAuthor(s): M.M. Gutierrez-Pacheco, A.T. Bernal-Mercado, F.J. Vazquez-Armenta, M.A. Martinez-Tellez, G.A. Gonzalez-Aguilar, J. Lizardi-Mendoza, T.J. Madera-Santana, F. Nazzaro, J.F. Ayala-ZavalaAbstractPathogenic bacteria use Quorum sensing (QS) to regulate the expression of virulence factors involved in plant tissue infection. Some of these factors are the production of biofilm, hydrolytic enzymes, toxins, and plasmids; therefore, the interruption of this system could be a useful tool to control plant tissue infections. This r...
Source: Physiological and Molecular Plant Pathology - April 9, 2019 Category: Molecular Biology Source Type: research

Functional analysis of elicitins and identification of cell wall proteins in Phytophthora cinnamomi
Publication date: Available online 8 April 2019Source: Physiological and Molecular Plant PathologyAuthor(s): M.T. Islam, H.I. Hussain, R. Russo, A. Chambery, A. Amoresano, A. Schallmey, W. Oßwald, P.P. Nadiminti, D.M. CahillAbstractPhytophthora cinnamomi Rands, a soil-borne oomycete plant pathogen, is a major threat to natural vegetation in many countries, especially Australia. The disease that it causes also has economic impacts on several agricultural and forestry systems worldwide. To investigate the virulence mechanisms of P. cinnamomi the roles of elicitins and elicitin-like proteins were examined using a susceptible...
Source: Physiological and Molecular Plant Pathology - April 8, 2019 Category: Molecular Biology Source Type: research

p-coumaric acid induces jasmonic acid-mediated phenolic accumulation and resistance to black rot disease in Brassica napus
Publication date: Available online 5 April 2019Source: Physiological and Molecular Plant PathologyAuthor(s): Md Tabibul Islam, Bok-Rye Lee, Van Hien La, Hyo Lee, Woo-Jin Jung, Dong-Won Bae, Tae-Hwan KimAbstractTo investigate p-coumaric acid (pCA)-induced plant resistance, the regulation of jasmonic acid (JA)-mediated phenylpropanoid biosynthesis pathway was assessed in response to pre-treatment with pCA or an inhibitor of 4-coumarate–CoA ligase (4CL), 3,4-(methylenedioxy) cinnamic acid (MDCA), in Brassica napus cultivar (cv. Mosa) following Xanthomonas campestris pv. campestris (Xcc) inoculation. At seven days post inocu...
Source: Physiological and Molecular Plant Pathology - April 6, 2019 Category: Molecular Biology Source Type: research

RSI1/FLD is a positive regulator for defense against necrotrophic pathogens
Publication date: Available online 6 April 2019Source: Physiological and Molecular Plant PathologyAuthor(s): Vijayata Singh, Deepjyoti Singh, Janesh Kumar Gautam, Ashis Kumar NandiAbstractThe reduced systemic immunity 1 (rsi1) mutant of Arabidopsis (allelic to fld; flowering locus D) is essential for systemic acquired resistance (SAR). RSI/FLD codes for putative histone-demethylase and modulates flowering and defense response. The rsi1/fld mutants are defective in systemic SA accumulation upon pathogen challenge and fail to respond to the SAR signals in the distal tissues. However, the role of RSI1/FLD in ethylene (ET)-jas...
Source: Physiological and Molecular Plant Pathology - April 6, 2019 Category: Molecular Biology Source Type: research

Salicylic acid deficient Nicotiana benthamiana attenuated virus induced gene silencing but did not affect transgene-induced posttranscriptional gene silencing nor general biogenesis of microRNAs
Publication date: Available online 5 April 2019Source: Physiological and Molecular Plant PathologyAuthor(s): Xiaohe Song, Xiaobao YingAbstractRNA interfering (RNAi) is a conserved mechanism in plants which plays a vital role in plant development, response to abiotic stress and biotic stress mediated by endogenous small RNAs and miRNAs as well as small RNAs originated from exogenic RNAs. Salicylic acid (SA) is an important signal in plant resistance to viral infection, functioning in R-gene mediated resistance as well as RNAi. NIbV3, Nicotiana benthamiana (N.benthamiana) transformed with nuclear inclusion b (NIb) gene of pl...
Source: Physiological and Molecular Plant Pathology - April 5, 2019 Category: Molecular Biology Source Type: research

The infection of grapes by Talaromyces rugulosus O1 and the role of Cell wall-degrading enzymes and ochratoxin A in the infection
This study provides useful information about the role of cell wall degrading enzymes and OTA in the infection of grapes by T. rugulosus O1. (Source: Physiological and Molecular Plant Pathology)
Source: Physiological and Molecular Plant Pathology - April 3, 2019 Category: Molecular Biology Source Type: research

Effects of transgenic glufosinate-tolerant rapeseed (Brassica napus L.) and the associated herbicide application on rhizospheric bacterial communities
Publication date: Available online 22 March 2019Source: Physiological and Molecular Plant PathologyAuthor(s): Tang Tang, GuiMin Chen, FuXia Liu, CuiPing Bu, Lei Liu, Zhao XiangXiangAbstractEffects of transgenic crops on soil microbial communities are important constituents in ecological risk assessment. The Illumina MiSeq sequencing method was employed to examine the effects of transgenic glufosinate-resistant rapeseed and the associated herbicide application on rhizospheric bacterial communities at different growth stages. Our results showed that both transgenic rapeseed and the application of herbicide Basta (active ingr...
Source: Physiological and Molecular Plant Pathology - March 23, 2019 Category: Molecular Biology Source Type: research

Effect of trisodium phosphate treatment on black spot of apple fruit and the roles of anti-oxidative enzymes
In this study, apple fruit (cv. Golden delicious) was used as the materials to study the effects of 0.25, 0.5, 1.0, 2.0 mg/mL trisodium phosphate (TSP) dipping treatment on lesion development of fruit inoculated with A. alternata. In vitro test was conducted to investigate the effect of TSP on mycelia growth of A. alternata. The optimal concentration of TSP was screened to assay its effect on the activity of anti-oxidative enzymes, the content of H2O2 and phenylpropane metabolites in apple fruit. The results showed that the different concentrations of TSP significantly inhibited mycelia growth of A. alternata and indicat...
Source: Physiological and Molecular Plant Pathology - March 16, 2019 Category: Molecular Biology Source Type: research

Extended persistence of Candidatus Liberibacter asiaticus (CLas) DNA in Huanglongbing-affected citrus tissue after bacterial death
Publication date: April 2019Source: Physiological and Molecular Plant Pathology, Volume 106Author(s): Ed Etxeberria, Pedro Gonzalez, Christopher Vincent, Arnold SchumannAbstractObjectiveWe aimed to determine the rate of Candidatus Liberibacter asiaticus (CLas) DNA disappearance from Huanglongbing (citrus greening disease)-affected citrus trees after bacterial death from heat treatment.ResultsUsing the leaf disc sampling method, we followed CLas qPCR Ct values in leaves of potted ‘Valencia’ orange trees after a heat-treatment that eliminated viable CLas. Although titer declined, CLas remained detectable 5 months after t...
Source: Physiological and Molecular Plant Pathology - March 12, 2019 Category: Molecular Biology Source Type: research

Genome-wide identification of Lysin-Motif Receptor-Like Kinase (LysM-RLK) gene family in Brachypodium distachyon and docking analysis of chitin/LYK binding
Publication date: Available online 9 March 2019Source: Physiological and Molecular Plant PathologyAuthor(s): Guzin Tombuloglu, Huseyin Tombuloglu, Emre Cevik, Hussain SabitAbstractLysin-Motif Receptor-Like Kinase (LysM-RLK) family proteins have critical function in plant-microbe interaction. During infection, common peptide domain of these proteins, namely LysM, interacts with bacterial gylcans or fungal chitins which triggers; (1) plant immune responses, (2) plant defense responses, or (3) plant-microorganism symbiotic interactions. Since pathogenic diseases cause severe annual loss in cereals, a better understanding of t...
Source: Physiological and Molecular Plant Pathology - March 10, 2019 Category: Molecular Biology Source Type: research

Piriformospora indica colonization reprograms plants to improved P-uptake, enhanced crop performance, and biotic/abiotic stress tolerance
Publication date: Available online 5 March 2019Source: Physiological and Molecular Plant PathologyAuthor(s): Mehtab Muhammad Aslam, Joseph Karanja, Suleiman Kehinde BelloAbstractPiriformospora indica is an endophytic fungus that promotes plant growth by establishing mutually beneficial alliances with plant roots reminiscent of arbuscular mycorrhizal fungi. It solubilizes soil inaccessible organic P to readily available Pi, thus, constitutes an indirect source of phosphate for plant growth under P limitation. In exchange, P. indica derives photosynthates (carbon) from host plant cell that is necessary for its metabolic proc...
Source: Physiological and Molecular Plant Pathology - March 5, 2019 Category: Molecular Biology Source Type: research