Extended persistence Candidatus Liberibacter asiaticus (CLas) DNA in Huanglongbing-affected citrus tissue after bacterial death
Publication date: Available online 5 March 2019Source: Physiological and Molecular Plant PathologyAuthor(s): Ed Etxeberria, Pedro Gonzalez, Christopher Vincent, Arnold SchumannAbstractObjectiveWe aimed to determine the rate of Candidatus Liberibacter asiaticus (CLas) DNA disappearance from Huanglongbing (citrus greening disease)-affected citrus trees after bacterial death from heat treatment.ResultsUsing the leaf disc sampling method, we followed CLas qPCR Ct values in leaves of potted ‘Valencia’ orange trees after a heat-treatment that eliminated viable CLas. Although titer declined, CLas remained detectable 5 months ...
Source: Physiological and Molecular Plant Pathology - March 5, 2019 Category: Molecular Biology Source Type: research

Induced changes in metabolic constituents of grapevine (Vitis vinifera L.) leaves infected with grapevine leafroll-associated virus-3
Publication date: April 2019Source: Physiological and Molecular Plant Pathology, Volume 106Author(s): Nargiz Sultanova, Nargiz Bayramova, Durna Aliyeva, Mina Rastgou, Irada HuseynovaAbstractGrapevine leafroll disease (GLD) is an economically important virus disease affecting grapes (Vitis spp.) in almost all grape-producing areas, but little is known about its effect on metabolic constitutes of affected grapes. Fifty-seven suspicious grapevine samples of various cultivars showing symptoms of leaf curl, vein greening, reduced leaf size, necrosis, mosaics or reddening and reduced fruit yield and quality were collected during...
Source: Physiological and Molecular Plant Pathology - March 4, 2019 Category: Molecular Biology Source Type: research

Papilla formation, defense gene expression and HR contribute to the powdery mildew resistance of the novel wheat line L699 carrying Pm40 gene
Publication date: Available online 1 March 2019Source: Physiological and Molecular Plant PathologyAuthor(s): X.L. Chang, L.Y. Luo, Y.P. Liang, Y.T. Hu, P.G. Luo, G.S. Gong, H.B. Chen, M.I. Khaskheli, T.G. Liu, W.Q. Chen, M. ZhangAbstractPowdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is one of the most destructive wheat diseases worldwide and causes severe yield losses in most wheat-growing areas. POWDERY MILDEW (Pm) genes are responsible for wheat resistance to Bgt. Recently, a wheat line L699 carrying a novel Pm40 gene which was originated from Elytrigia inermedium, was developed and showed high resista...
Source: Physiological and Molecular Plant Pathology - March 1, 2019 Category: Molecular Biology Source Type: research

The er2 gene resistance against powdery mildew infection is associated with enhanced antioxidative protection and defense gene expression
Publication date: Available online 27 February 2019Source: Physiological and Molecular Plant PathologyAuthor(s): Sheetal M. Bhosle, Nitinkumar Marathe, Ragiba MakandarAbstractThe present study was aimed at analyzing host responses of JI-2480 a resistant pea genotype carrying a recessive gene er2 and Arkel-a commercial cultivar susceptible to powdery mildew infection caused by Erysiphe pisi. The cellular responses were analyzed through biochemical, histo-chemical and defense gene expression in a time-course manner. The reactive oxygen species (ROS) scavenging enzymes showed higher activity in JI-2480 post-inoculation compar...
Source: Physiological and Molecular Plant Pathology - February 28, 2019 Category: Molecular Biology Source Type: research

In silico identification of copper-binding proteins of Xanthomonas translucens pv. undulosa for their probable role in plant-pathogen interactions
This study will further assist towards the understanding of the copper homeostasis in Xtu and the probable roles of copper-binding proteins in plant-pathogen interactions.Graphical abstract (Source: Physiological and Molecular Plant Pathology)
Source: Physiological and Molecular Plant Pathology - February 28, 2019 Category: Molecular Biology Source Type: research

Quantitative trait loci mapping of leaf rust resistance in tetraploid alfalfa
This study suggests that leaf rust resistance in alfalfa is most likely incomplete and has polygenic inheritance. Comparative genome analysis of QTL regions using sequences of significant SNPs revealed homology to M. truncatula sequences that were previously reported for their role in plant defense mechanisms. Validation of the QTLs in different genetic backgrounds as well as fine mapping of the QTL regions would be the next step of this research. The markers presented in this study would be valuable resources for alfalfa genetic improvement via marker-assisted selection (MAS). (Source: Physiological and Molecular Plant Pathology)
Source: Physiological and Molecular Plant Pathology - February 26, 2019 Category: Molecular Biology Source Type: research

Changes in phenylpropanoid pathway gene expression in roots and leaves of susceptible and resistant Brassica napus lines in response to Plasmodiophora brassicae inoculation
Publication date: Available online 25 February 2019Source: Physiological and Molecular Plant PathologyAuthor(s): Solmaz Irani, Christopher D. Todd, Yangdou Wei, Peta C. Bonham-SmithAbstractClubroot, caused by the obligate parasite Plasmodiophora brassicae, is currently one of the most severe diseases of the Brassicaceae. As a soil-borne pathogen, P. brassicae infects the roots of host plants, inducing the formation of galls and negatively affecting plant growth and productivity. Roots and leaves of two clubroot susceptible and one resistant Brassica napus lines, inoculated with P. brassicae spores, were monitored for disea...
Source: Physiological and Molecular Plant Pathology - February 25, 2019 Category: Molecular Biology Source Type: research

Effects of Penicillium infection on the expression and activity of CDPK2 in postharvest Hami melon treated with calcium chloride
Publication date: Available online 16 February 2019Source: Physiological and Molecular Plant PathologyAuthor(s): Ming Ning, Fengxian Tang, Qin Zhang, Xinxin Zhao, Liping Yang, Wenchao Cai, Chunhui ShanAbstractCalcium ions are second messengers in plant signal transduction involved in plant growth and development and the response to stress. Calcium-dependent protein kinase (CDPK) is calcium ion receptor functioning in plant disease resistance. Herein, we investigated changes in CDPK activity and resistance to Penicillium in postharvest Hami melons after different treatments. The non-infection group was permeated with calciu...
Source: Physiological and Molecular Plant Pathology - February 17, 2019 Category: Molecular Biology Source Type: research

In silico identification of copper-binding proteins of Xanthomonas translucens pv.undulosa for their probable role in plant-pathogen interactions
This study will further assist towards the understanding of the copper homeostasis in Xtu and the probable roles of copper-binding proteins in plant-pathogen interactions.Graphical abstract (Source: Physiological and Molecular Plant Pathology)
Source: Physiological and Molecular Plant Pathology - February 16, 2019 Category: Molecular Biology Source Type: research

Assessment of genetic diversity and population structure of Magnaporthe oryzae causing rice blast disease using SSR markers
Publication date: Available online 12 February 2019Source: Physiological and Molecular Plant PathologyAuthor(s): Manoj Kumar Yadav, S. Aravindan, S. Raghu, S.R. Prabhukarthikeyan, U. Keerthana, Umakanta Ngangkham, D. Pramesh, Amrita Banerjee, Totan Adak, Meera Kumari Kar, C. Parameswaran, Rupesh Deshmukh, Jitendra Kumar Tiwari, Mihir Ranjan Mohanty, P.C. RathAbstractRice blast caused by Magnaporthe oryzae is one of the most destructive diseases of rice in the world. The blast fungus shows a high degree of variability in the field and limited information is available on its genetic diversity and population structure in Indi...
Source: Physiological and Molecular Plant Pathology - February 13, 2019 Category: Molecular Biology Source Type: research

Development of novel methods for functional evaluation of the signal peptide of secreted protein
Publication date: Available online 8 February 2019Source: Physiological and Molecular Plant PathologyAuthor(s): Songlin Xie, Yufu Wang, Wei Wei, Yang Lin, Weixiao Yin, Chaoxi LuoAbstractSecreted proteins play critical roles in plant-microbe interactions, in which they manipulate host metabolism, defense responses, physiology, etc. Improved methods for identifying pathogens secreted proteins will accelerate biological discoveries in cell signaling and cellular communication. Bioinformatics tools have been used to predict secreted proteins from genomic data, but few methods have been used in experimental identification of se...
Source: Physiological and Molecular Plant Pathology - February 8, 2019 Category: Molecular Biology Source Type: research

Redox- and bio-activity of apocynin (acetovanillone) in tobacco, a plant phenolic that alleviates symptoms of autoimmune diseases in animals
Publication date: Available online 8 February 2019Source: Physiological and Molecular Plant PathologyAuthor(s): C. Jacyn Baker, Norton M. Mock, Andrey A. AveryanovAbstractApocynin (APO) was shown to be the major constituent of early herbal medicines used to reduce painful suffering from many ailments. Because of its proven utility in rat models, it is now an active candidate for treating humans for several major autoimmune diseases such as Alzheimer's, arthritis, and cardiovascular diseases. In plant research, APO is referred to as acetovanillone, a secondary metabolite that is induced in the leaf apoplast during bacterial...
Source: Physiological and Molecular Plant Pathology - February 8, 2019 Category: Molecular Biology Source Type: research

Dynamic changes in common metabolites and antioxidants during Penicillium expansum-apple fruit interactions
Publication date: Available online 4 February 2019Source: Physiological and Molecular Plant PathologyAuthor(s): Aleksandra Žebeljan, Ivana Vico, Nataša Duduk, Bojana Žiberna, Andreja Urbanek KrajncAbstractPenicillium expansum-infected apple fruit resulted in a disrupted balance of primary metabolites and impacted the ascorbate–glutathione cycle. The initial decline in fructose, malic acid, shikimic acid and total ascorbate content (6 hpi) was followed by a significant increase in malic acid (24 hpi) and total glutathione (3 dpi). Significant increase in sucrose was accompanied by a decline in glucose and ascorbic acid...
Source: Physiological and Molecular Plant Pathology - February 5, 2019 Category: Molecular Biology Source Type: research

Editorial Board
Publication date: January 2019Source: Physiological and Molecular Plant Pathology, Volume 105Author(s): (Source: Physiological and Molecular Plant Pathology)
Source: Physiological and Molecular Plant Pathology - February 1, 2019 Category: Molecular Biology Source Type: research

Seasonal variation and detection frequency of Candidatus Liberibacter asiaticus in Binchuan, Yunnan province China
In this study, we investigated the seasonal variations in the population dynamics of Clas and assessed the possible genetic variations among the identified isolates. We conducted a disease survey in tangerine citrus groves affected with HLB in Binchuan county, Yunnan province, China, on a monthly basis over a period of one year. The collected leaf samples were analyzed every month via nested PCR, targeting the 16S rRNA and the β-operon ribosomal protein-encoding genes of Clas. The abundance of the HLB-causing pathogen changes several times, with high temperatures in July 2017 yielding low pathogen abundance, followed by a...
Source: Physiological and Molecular Plant Pathology - February 1, 2019 Category: Molecular Biology Source Type: research