Assessment of genetic diversity and population structure of Magnaporthe oryzae causing rice blast disease using SSR markers

Publication date: Available online 12 February 2019Source: Physiological and Molecular Plant PathologyAuthor(s): Manoj Kumar Yadav, S. Aravindan, S. Raghu, S.R. Prabhukarthikeyan, U. Keerthana, Umakanta Ngangkham, D. Pramesh, Amrita Banerjee, Totan Adak, Meera Kumari Kar, C. Parameswaran, Rupesh Deshmukh, Jitendra Kumar Tiwari, Mihir Ranjan Mohanty, P.C. RathAbstractRice blast caused by Magnaporthe oryzae is one of the most destructive diseases of rice in the world. The blast fungus shows a high degree of variability in the field and limited information is available on its genetic diversity and population structure in India. Twenty five polymorphic SSR markers were used to investigate the genetic diversity and population structure of 96 blast isolates collected from Odisha and Chhattisgarh region. The markers yielded a total of 110 alleles and number of alleles per locus varied from 2 to 8. The gene diversity ranged from 0.02 to 0.49 whereas, major allele frequency varied from 0.50 to 0.98. The PIC value varied from 0.02 to 0.37 with a mean value of 0.29. Cluster analysis grouped the blast isolates into four distinct major clusters. The high level of genetic diversity was observed with ninety one haplotypes and nucleotide diversity of 0.293. The principal coordinate and structure analysis identified two genetic clusters of M. oryzae isolates with some degree of distinctness according to affected plant parts. Analysis of molecular variance revealed more genetic variation withi...
Source: Physiological and Molecular Plant Pathology - Category: Molecular Biology Source Type: research