Physiological and Molecular Plant Pathology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Introduction
Publication date: Available online 22 January 2019Source: Physiological and Molecular Plant PathologyAuthor(s): Angela Obiageli Eni (Source: Physiological and Molecular Plant Pathology)
Source: Physiological and Molecular Plant Pathology - January 23, 2019 Category: Molecular Biology Source Type: research

Jasmonic acid and nitric oxide protects naranjilla (Solanum quitoense) against infection by Fusarium oxysporum f. sp. quitoense by eliciting plant defense responses
Publication date: Available online 14 January 2019Source: Physiological and Molecular Plant PathologyAuthor(s): Ana Cristina Ávila, José Ochoa, Karina Proaño, M. Carmen MartínezAbstractNaranjilla (Solanum quitoense) is an Andean tropical fruit of the solanaceae family with high nutritional value. Its cultivation is gravely affected by the lethal disease produced by Fusarium oxysporum f.sp. quitoense, causing severe economic losses. In this work we studied the possibility of boosting the plants’ natural defenses to the fungus by using plant hormones. Our results show that spraying jasmonic acid or sodium nitroprusside...
Source: Physiological and Molecular Plant Pathology - January 15, 2019 Category: Molecular Biology Source Type: research

Comparative transcriptomic analysis reveals different responses of Arabidopsis thaliana roots and shoots to infection by Agrobacterium tumefaciens in a hydroponic co-cultivation system
In this study, A. thaliana were co-cultivated (infected) with A. tumefaciens in a sterile hydroponic system for 8 hours, and the transcriptome profiles of root and shoot were determined by microarray analysis. Overall, hundreds of genes involved in plant responses to stress, regulation of transcription, signal transduction or plant metabolism are differentially expressed in roots and shoots upon A. tumefaciens infection. In total, 323 genes were up-regulated and 226 were down-regulated by a log2 transformed fold change of at least 2 in roots upon A. tumefaciens infection, as compared with mock-inoculated plants. In the sho...
Source: Physiological and Molecular Plant Pathology - January 12, 2019 Category: Molecular Biology Source Type: research

Efficacy of certain bioagents on patho-physiological characters of wheat plants under wheat leaf rust stress
Publication date: Available online 4 January 2019Source: Physiological and Molecular Plant PathologyAuthor(s): R.I. Omara, Gabr El-Kot A, Fawzya M. Fadel, A.A. Khaled Abdelaal, Eman M. SalehAbstractLeaf rust caused by Puccinia triticina f.sp. tritici is the most important serious diseases attacking wheat plants. The efficacy of bioagents, i.e. Bacillus subtilis, Bacillus chitinospours, Bacillus pumilus, Trichoderma viride and Trichoderma harzianum to control leaf rust was studied. The bioagents were sprayed before and after inoculation with P. triticina by 24 hours during 2016/17 and 2017/18 growing seasons. Our results sh...
Source: Physiological and Molecular Plant Pathology - January 5, 2019 Category: Molecular Biology Source Type: research

Reprint of “Molecular and phenotypic characterization of pathogenic fungal strains isolated from ginseng root rot”
Publication date: Available online 21 November 2018Source: Physiological and Molecular Plant PathologyAuthor(s): Kaliannan Durairaj, Palanivel Velmurugan, Kaliyappan Amballandi Vedhanayakisri, Woo-Suk Chang, Palaninaicker Senthilkumar, Kyung-Min Choi, Jeong-Ho Lee, Byung-Taek OhAbstractIn the present study, we investigated the diversity of pathogenic fungal strain isolated from infected Korean ginseng root rot and tested their morphology, molecular characterization, and pathogenic impact. From the results, we have identified different shapes of fungal conidia such as cylindrical, conical, bell and convex shape. The molecul...
Source: Physiological and Molecular Plant Pathology - January 4, 2019 Category: Molecular Biology Source Type: research

Trichoderma asperellum T1 mediated antifungal activity and induced defense response against leaf spot fungi in lettuce (Lactuca sativa L.)
Publication date: April 2019Source: Physiological and Molecular Plant Pathology, Volume 106Author(s): Burhanah Baiyee, Shin-ichi Ito, Anurag SunpapaoAbstractLeaf spot, caused by Corynespora cassiicola and Curvularia aeria, is a major disease of lettuce that results in the reduction of the quality and the quantity of lettuce production. Primary in vitro examination revealed that Trichoderma sp. T1 inhibited the mycelial growth of C. cassiicola and C. aeria by 83.79% and 85.71%, respectively, in dual culture assays. Gel diffusion showed cell-free culture filtrate of Trichoderma sp. T1 inhibited C. cassiicola and C. aeria by ...
Source: Physiological and Molecular Plant Pathology - January 1, 2019 Category: Molecular Biology Source Type: research

Defense responses against the sorghum anthracnose pathogen in leaf blade and midrib tissue of johnsongrass and sorghum
Publication date: Available online 21 December 2018Source: Physiological and Molecular Plant PathologyAuthor(s): Ezekiel Ahn, Louis K. Prom, Gary Odvody, Clint MagillAbstractThree isolates of Colletotrichum sublineolum P. Henn were inoculated on leaf blades and midribs of twenty-six johnsongrass cultivars with two sorghum cultivars, BTx 623 and SC 748-5 by using an excised leaf method. Phenotypic host responses from no response to severe infection, were observed. Some johnsongrass cultivars and BTx 623 showed different responses between the two tissues. Expression of six defense response related genes were measured using R...
Source: Physiological and Molecular Plant Pathology - December 21, 2018 Category: Molecular Biology Source Type: research

Responses of Fuji (Malus domestica) and Shandingzi (Malus baccata) apples to Marssonina coronaria infection revealed by comparative transcriptome analysis
In this study, the responses of Fuji and Shandingzi to M. coronaria infection were studied by comparative transcriptome analysis. As a result, 4898 and 2897 differentially expressed genes (DEGs) in Fuji and Shandingzi, respectively, were identified. Gene Ontology (GO) enrichment analysis of the co-expressed DEGs showed that 25 significant terms were enriched, and the unique DEGs in Shandingzi were mainly associated with the biotic stimulus response (GO: 0009607), defense response (GO: 0006952), stress response (GO: 0006950) and oxidoreductase activity (GO: 0016491). Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment...
Source: Physiological and Molecular Plant Pathology - December 15, 2018 Category: Molecular Biology Source Type: research

Induced changes in metabolic constituents of grapevine (Vitisvinifera L.) leaves infected with grapevine leafroll-associated virus-3
Publication date: April 2019Source: Physiological and Molecular Plant Pathology, Volume 106Author(s): Nargiz Sultanova, Nargiz Bayramova, Durna Aliyeva, Mina Rastgou, Irada HuseynovaAbstractGrapevine leafroll disease (GLD) is an economically important virus disease affecting grapes (Vitis spp.) in almost all grape-producing areas, but little is known about its effect on metabolic constitutes of affected grapes. Fifty-seven suspicious grapevine samples of various cultivars showing symptoms of leaf curl, vein greening, reduced leaf size, necrosis, mosaics or reddening and reduced fruit yield and quality were collected during...
Source: Physiological and Molecular Plant Pathology - December 12, 2018 Category: Molecular Biology Source Type: research

Methylation-sensitive amplified polymorphism analysis of resistant and susceptible interactions of cucumber with Podosphaera xanthii
Publication date: Available online 8 December 2018Source: Physiological and Molecular Plant PathologyAuthor(s): Namdar Moradi, Ali Rezaei, Bahman Bahramnejad, Paul H. GoodwinAbstractCytosine methylation alterations play key roles in gene regulation in plants interacting with biotrophic pathogens. For resistant and susceptible interactions of cucumber with the powdery mildew pathogen, Podosphaera xanthii, methylation of DNA cytosines were monitored by methylation-sensitive amplified polymorphism (MSAP) analysis from immediately prior to 96 h post inoculation (hpi). In both resistant and susceptible interactions, methylati...
Source: Physiological and Molecular Plant Pathology - December 9, 2018 Category: Molecular Biology Source Type: research

Purification and in vitro activities of a chitinase-modifying protein from the corn ear rot pathogen Stenocarpella maydis.
Publication date: Available online 8 December 2018Source: Physiological and Molecular Plant PathologyAuthor(s): Todd A. Naumann, Neil P.J. PriceAbstractStenocarpella maydis is the most common corn ear rot pathogen throughout the world. We previously reported that this fungus secretes a protease, Stm-cmp, which functions as a chitinase-modifying protein (CMP) to truncate corn ChitA chitinase. In the current manuscript we describe the purification of Stm-cmp from fungal cultures and characterize its activity using in vitro protease assays. Hydrophobic interaction chromatography was a key purification step as Stm-cmp bound th...
Source: Physiological and Molecular Plant Pathology - December 8, 2018 Category: Molecular Biology Source Type: research

Induced changes ın metabolıc constıtuents of grapevıne (vıtıs vınıfera l.) Leaves ınfected wıth grapevıne leafroll-assocıated vırus-3
Publication date: Available online 6 December 2018Source: Physiological and Molecular Plant PathologyAuthor(s): Nargiz Sultanova, Nargiz Bayramova, Durna Aliyeva, Mina Rastgou, Irada HuseynovaAbstractGrapevine leafroll disease (GLD) is an economically important virus disease affecting grapes (Vitis vinifera L.) in almost all grape-producing areas, but little is known about its effect on metabolic constitutes of affected grapes. Fifty-seven suspicious grapevine samples of various cultivars showing symptoms of leaf curl, vein greening, reduced leaf size, necrosis, mosaics or reddening and reduced fruit yield and quality were...
Source: Physiological and Molecular Plant Pathology - December 6, 2018 Category: Molecular Biology Source Type: research

Molecular cloning and functional analysis of a necrosis and ethylene inducing protein (NEP) from Ganoderma boninense
In this study, we reported the cloning and characterization of a transcript encoding NEP from Ganoderma boninense which belongs to a family of fungi in Ganodermataceae that cause serious infections of cacao, rubber, tea, coffee and palms. The open reading frame (ORF) encoding NEP in G. boninense (GbNEP) was cloned by 5’ and 3’ rapid amplification of cDNA ends (RACE) PCR. The transcript abundance of GbNEP increased in fungal culture treated with salicyclic acid and jasmonic acid, respectively. The soluble recombinant GbNEP expressed in Escherichia coli BL21(DE3)pLysS was able to induce necrosis in tobacco and tomato but...
Source: Physiological and Molecular Plant Pathology - December 6, 2018 Category: Molecular Biology Source Type: research

Biostimulant and fungicidal effects of phosphite assessed by GC-TOF-MS analysis of potato leaf metabolome
Publication date: Available online 3 December 2018Source: Physiological and Molecular Plant PathologyAuthor(s): Lin Wu, Xingxi Gao, Fagang Xia, Jyoti Joshi, Tudor Borza, Gefu Wang-PruskiAbstractPhosphite (Phi), an analog of phosphate, is increasingly used worldwide as fungicide, fertilizer and plant biostimulant. Studies using various plant systems provided insights on how Phi is translocated in plants and on its mode of action against pathogens. However, little evidence is present at the metabolite level, of the compounds present in plants that are responsible in the enhanced resistance to pathogens. As Phi is used to con...
Source: Physiological and Molecular Plant Pathology - December 4, 2018 Category: Molecular Biology Source Type: research

Development of Potato virus Y (PVY) resistant pepper (Capsicum annuum L.) lines using marker-assisted selection (MAS)
In this study, PVY resistant F2 pepper lines were developed from local germplasm carrying recessive genes (pvr21 and pvr22) using marker-assisted selection (MAS). The F1 and F2 generations were assessed for the presence of resistant/susceptible alleles (pvr2+/pvr21/pvr22) at the pvr2-elF4E locus using the tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) procedure. Our results show that ARMS-PCR can be used to successfully screen pepper genotypes for alleles that confer PVY resistance thereby contributing to the improvement of pepper production. (Source: Physiological and Molecular Plant Pathology)
Source: Physiological and Molecular Plant Pathology - December 4, 2018 Category: Molecular Biology Source Type: research