Development of Potato virus Y (PVY) resistant pepper (Capsicum annuum L.) lines using marker-assisted selection (MAS)

In this study, PVY resistant F2 pepper lines were developed from local germplasm carrying recessive genes (pvr21 and pvr22) using marker-assisted selection (MAS). The F1 and F2 generations were assessed for the presence of resistant/susceptible alleles (pvr2+/pvr21/pvr22) at the pvr2-elF4E locus using the tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) procedure. Our results show that ARMS-PCR can be used to successfully screen pepper genotypes for alleles that confer PVY resistance thereby contributing to the improvement of pepper production.
Source: Physiological and Molecular Plant Pathology - Category: Molecular Biology Source Type: research