Biological control of Sclerotium fruit rot of snake fruit and stem rot of lettuce by Trichoderma sp. T76-12/2 and the mechanisms involved

Publication date: Available online 10 April 2019Source: Physiological and Molecular Plant PathologyAuthor(s): Prisana Wonglom, Wilailuck Daengsuwan, Shin-ichi Ito, Anurag SunpapaoAbstractFruit rot of snake fruit and stem rot of lettuce caused by Sclerotium sp. SZ01 and LS01, respectively, are major diseases causing devastating crop losses. Tichoderma species has been known as an effective biological control agent (BCA) against several plant disease pathogens. This research aimed to select an effective Trichoderma species for controlling both diseases. Five Trichoderma spp. isolates were subjected to primary screening against Sclerotium sp. LS01 and SZ01 by dual culture test. Trichoderma sp. T76-12/2 showed significant inhibitory effects on mycelial growth of Sclerotium sp. LS01 and SZ01 with 81.48% and 78.33% inhibition. The Trichoderma sp. T76-12/2 caused discoloration of Sclerotium sp. mycelia at the contact point. Volatile antifungal bioassay revealed Trichoderma sp. T76-12/2 was superior in inhibiting the growth of Sclerotium sp. by up to 44.44% for LS01 and by 59.52% for SZ01. Gas chromatography-mass spectrometry (GC/MS) was conducted to resolve the volatile organic compounds emitted by Trichoderma sp. T76-12/2, and the results revealed presence of antifungal compounds including alcohol (phenylethyl alcohol) and sesquiterpene (epi-cubenol). Enzyme assay of cell-free culture filtrate showed that the activity of β-1,3-glucanase was higher than that in control. Application...
Source: Physiological and Molecular Plant Pathology - Category: Molecular Biology Source Type: research