p-coumaric acid induces jasmonic acid-mediated phenolic accumulation and resistance to black rot disease in Brassica napus

Publication date: Available online 5 April 2019Source: Physiological and Molecular Plant PathologyAuthor(s): Md Tabibul Islam, Bok-Rye Lee, Van Hien La, Hyo Lee, Woo-Jin Jung, Dong-Won Bae, Tae-Hwan KimAbstractTo investigate p-coumaric acid (pCA)-induced plant resistance, the regulation of jasmonic acid (JA)-mediated phenylpropanoid biosynthesis pathway was assessed in response to pre-treatment with pCA or an inhibitor of 4-coumarate–CoA ligase (4CL), 3,4-(methylenedioxy) cinnamic acid (MDCA), in Brassica napus cultivar (cv. Mosa) following Xanthomonas campestris pv. campestris (Xcc) inoculation. At seven days post inoculation with Xcc, necrotic lesions decreased in response to pCA pre-treatment. JA content and expression of signaling genes (COI1 and PDF1.2) were enhanced in pCA-pre-treated plants, along with an increase in the MYB transcriptional factor, production of anthocyanin pigment 1 (PAP1), which regulates phenylpropanoid biosynthesis in Xcc-inoculated plants. Following Xcc inoculation, the accumulation of epigallocatechin and epigallocatechin gallate was increased in soluble forms; however, induced accumulation of pCA, ferulic acid, and sinapic acid was observed in both soluble and cell wall-bound forms in response to pCA pre-treatment, whereas this decreased in control or MDCA pre-treated plants. The expression of phenylpropanoid biosynthesis-related genes was upregulated in plants pre-treated with pCA, whereas it decreased or did not change in control and MDCA pr...
Source: Physiological and Molecular Plant Pathology - Category: Molecular Biology Source Type: research