Analysis of rutin, β‐carotene, and lutein content and evaluation of antioxidant activities of six edible leaves on free radicals and reactive oxygen species
AbstractThe antioxidant potency of soluble and bound phenolic extracts of freeze ‐dried six leafy vegetables was investigated using in vitro systems; singlet oxygen scavenging, hydroxyl radical scavenging, nitric oxide radical scavenging, and ABTS radicals assays. Further rutin, lutein, and β‐carotene content of leaves were analysed. The results revealed that both soluble a nd bound phenolic extracts have exhibited antioxidant potential toward free radicals and reactive oxygen species concerned. However, soluble extracts showed a higher content of phenolics and flavonoids than the bound counterpart. The highest rutin,...
Source: Journal of Food Biochemistry - October 24, 2018 Category: Food Science Authors: K. D. P. P. Gunathilake,
K. K. D. S. Ranaweera,
H. P. V. Rupasinghe Tags: FULL ARTICLE Source Type: research
Characterization of Vicia (Fabaceae) seed water extracts with potential immunomodulatory and cell antiproliferative activities
AbstractVicia seeds represent an underutilized source of nutritional and functional components. Besides being rich in proteins, they are also rich in polyphenols, free amino acids, and lectins. These components are enriched in a by ‐product of the production of protein isolates, a pH 4 soluble extract. Extracts from sixVicia have been analyzed, and NO radical scavenging and antiproliferative activities have been determined. Canavanine was the main free amino acid, while gallic acid derivatives were the most abundant of polyphenols. Fractionation of the extracts by ultrafiltration rendered a lectin ‐free fraction that e...
Source: Journal of Food Biochemistry - October 24, 2018 Category: Food Science Authors: Cristina Meg ías,
Isabel Cortés‐Giraldo,
Julio Girón‐Calle,
Manuel Alaiz,
Javier Vioque Tags: FULL ARTICLE Source Type: research
Synergistic effect of selenium and UV ‐B radiation in enhancing antioxidant level of wheatgrass grown from selenium rich wheat
In this study, exposure of Se‐rich wheatgrass to UV‐B radiation has exhibited a significant increase in different ant ioxidant properties. Thus, wheatgrass naturally enriched with selenium can be an effective source of Se supplementation along with other nutritional benefits that it facilitates. (Source: Journal of Food Biochemistry)
Source: Journal of Food Biochemistry - October 24, 2018 Category: Food Science Authors: Sumit K. Jaiswal,
Ranjana Prakash,
Anatoly V. Skalny,
Margarita G. Skalnaya,
Andrei R. Grabeklis,
Anastasia A. Skalnaya,
Alexey A. Tinkov,
Feng Zhang,
Xiong Guo,
N. Tejo Prakash Tags: FULL ARTICLE Source Type: research
Phenolic, tannin, antioxidant, color, and sensory attributes of Barhi date (Phoenix dactylifera) fruit stored in modified atmosphere packages
AbstractModified atmosphere packaging (MAP) was evaluated for total phenolic contents (TPC), antioxidant, tannin, color, and sensory properties of fresh Barhi dates. Polypropylene (PP) and polyethylene (PE) with different perforations (0 [H0], 50 µm [H3], 100 µm [H6], and 150 µm [H9]) were evaluated for storing fresh Barhi dates for up to 45 days at 1 to 25°C temperature. MAP significantly (p ≤ .05) affected the properties investigated as compared to control. PE dates have higher (p ≤ .05) TPC than PP dates, whereas other properties remained largely unaffected by packaging material. TPC and antioxidant acti...
Source: Journal of Food Biochemistry - October 24, 2018 Category: Food Science Authors: Omer N. Alsawmahi,
Fahad Al ‐Juhaimi,
Abdullah M. Alhamdan,
Kashif Ghafoor,
Oladipupo Q. Adiamo,
Isam A. Mohamed Ahmed,
Bakri H. Hassan,
Kheled A. Ehmed,
Elfadil E. Babiker,
Diaeldin Abdelkarim,
Mahmoud Younis,
Nasser Alashmawe Tags: FULL ARTICLE Source Type: research
Metabolomics and chemometric study for identification of acetylcholinesterase inhibitor(s) from the flower extracts of Nymphaea pubescens
AbstractInhibitors of acetylcholinesterase (AChE) are used in the treatment of Alzheimer's disease. Initial screening revealed that the edible flowers ofN. pubescens inhibited AChE. This finding motivated us to identify the contributory bioactive metabolite(s) responsible for inhibition AChE using Gas Chromatography ‐ Mass Spectrometry based metabolomics and chemometric approach. The metabolite levels as well as AChE inhibitory activity varied in different flower samples collected from different districts of West Bengal in the monsoon season. Different multivariate analyses differentiated the extracts with lo w activity ...
Source: Journal of Food Biochemistry - October 24, 2018 Category: Food Science Authors: Jayashree Acharya,
Mainak Dutta,
Koel Chaudhury,
Bratati De Tags: FULL ARTICLE Source Type: research
Production and immobilization of lipase PCL and its application in synthesis of α‐linolenic acid‐rich diacylglycerol
This study examined the production, immobilization of lipase PCL, and its application in the synthesis of diacylglycerol by esterification of α‐linolenic acid with glycerol. The resin ECR880 6 was selected as an effective support for the immobilization of lipase PCL. Fourier transform infrared and Laser scanning confocal microscope analysis proved that the lipase was successfully immobilized on the resin. Compared with the free PCL, the immobilized one exhibited higher temperature toler ance. Under optimized reaction conditions, a DAG content of 54.49% were obtained. After further purified by molecular distillation, the...
Source: Journal of Food Biochemistry - October 24, 2018 Category: Food Science Authors: Nan Liu,
Daoming Li,
Weifei Wang,
Frank Hollmann,
Long Xu,
Yunjian Ma,
Bo Yang,
Weidong Bai,
Xiaotao Sun,
Yonghua Wang Tags: FULL ARTICLE Source Type: research
Neuroprotective effect of dietary black seed flour on key enzymes linked with neuronal signaling molecules in rats exposed to mixture of environmental metals
AbstractThe study examined the neuroprotective effect of dietary black seed flour (D ‐BSF) in rats exposed to mixed‐metals. Animals were divided into six groups (n = 8): normal control rats; normal rats treated with 10 and 20% black seed flour (D‐BSF), respectively; intoxicated rat; intoxicated rats treated with 10 and 20% D‐BSF, respectively for 14 days. Our results revealed that neuronal signaling molecules including acetylcholinesterase (AChE), but rylcholinesterase (BuChE), adenosine deaminase (ADA), monoamine oxidase‐A (MAO‐A) activities, and malonaldehyde (MDA) levels in cerebral cortex were significa...
Source: Journal of Food Biochemistry - October 24, 2018 Category: Food Science Authors: J. K. Akintunde,
A. E. Irondi,
E. O. Ajani,
T. V. Olayemi Tags: FULL ARTICLE Source Type: research
In vivo wound healing potential and HPLC ‐PDA‐ESI‐MS profiling of Zizyphus lotus L. (Desf.) leaves methanol extract
This study was performed to evaluate the wound healing potential and antioxidant effect of methanol extract ofZizyphus lotus leaves (MZL), and to identify its phenolic profile by HPLC ‐PDA‐ESI‐MS. Wound healing activity was assessed in vivo using excision model. Biochemical and histopathological analyses were conducted. In vitro antioxidant potential of MZL was tested using 2,2‐diphenyl‐1‐picrylhydrazyl and β‐carotene bleaching methods. HPLC‐PDA‐ESI‐MS was us ed for the phenolic profiling. Animals treated with MZL ointment showed a significant reduction in epithelialization time and high contraction ra...
Source: Journal of Food Biochemistry - October 24, 2018 Category: Food Science Authors: Halima Saiah,
Meriem Mokhtar,
Wassila Saiah,
Ahmed Aichouni,
Fatima Zohra EL Kebir,
Rachida Allem Tags: FULL ARTICLE Source Type: research
Bioactivity ‐guided isolation of antidiabetic principles from the methanolic leaf extract of Bryophyllum pinnatum
AbstractThe antidiabetic principles from methanolic leaf extract ofBryophyllum pinnatum were isolated. The leaf extract was fractionated on silica gel using column chromatography and identified using nuclear magnetic resonance (NMR) spectrometry. The ethylacetate fraction of the partitioned methanolic extract ofB. pinnatum lowered blood glucose of alloxan ‐induced diabetic rats and inhibited α‐amylase and α‐glucosidase withIC50 137.89 and 110.15 µg/mL, respectively. In addition to lowering blood glucose, fractions A and B inhibited α‐amylase withIC50 57.43 and 43.84 µg/mL and α‐glucosidase with IC50 11....
Source: Journal of Food Biochemistry - October 24, 2018 Category: Food Science Authors: O. B. Ibitoye,
K. A. Olofinsan,
K. Teral ı,
U. M. Ghali,
T. O. Ajiboye Tags: FULL ARTICLE Source Type: research
Curcumin suppresses pro ‐inflammatory adhesion response in Human Umbilical Vein Endothelial Cells
AbstractAtherosclerosis, a progressive pathological disorder, is a leading cause of human morbidity and mortality worldwide. The expression of cell adhesion molecules by the endothelium and the attachment of monocytes to endothelium may play a pivotal role in the early atherogenic process. Curcumin, a polyphenol obtained from the root of turmeric, is the main component of curry powder. Previous studies indicated that curcumin exhibits a variety of pharmacological effects, including anti ‐inflammatory, antitumor, antioxidant, and anti‐hypertensive properties. In the current study, we found that curcumin could suppress t...
Source: Journal of Food Biochemistry - October 24, 2018 Category: Food Science Authors: Weng ‐Cheng Chang,
Ya‐Mei Yu,
An‐Chin Cheng Tags: FULL ARTICLE Source Type: research
Effect of high ‐pressure processing on the polysaccharides content and antioxidant activity of fresh Dendrobium officinale juice
AbstractFreshDendrobium officinale possessed higher activities compared with the dried ones. High ‐pressure processing (HPP) could effectively enhance the content and activities ofDendrobium officinale polysaccharides (DOPS) which is the main active ingredients of freshDendrobium officinale juice (FDOJ). Pressure, temperature, and holding time were optimized using response surface methodology to enhance DOPS content. The DOPS molecular weights (Mw) compositions and antioxidantive activities of FDOJ under the optimal HPP conditions (HDOJ) and FDOJ samples were also determined. The result indicated that under the optimal c...
Source: Journal of Food Biochemistry - October 24, 2018 Category: Food Science Authors: Juan Liang,
Sai Guo,
An Zhou,
Ai Ling Hui,
Kai Zong,
Nian Jun Yu,
Dai Yin Peng Tags: FULL ARTICLE Source Type: research
Bioactive compounds and antioxidant activity of papaya inoculated with Colletotrichum gloeosporioides as affected by hot water –calcium chloride
This study was performed to determine the effect of hot water (HW; 48 °C/20 min), calcium chloride (Ca; 1%/20 min), and their combination (HW‐Ca) in bioactive compounds and antioxidant activity of papaya inoculated withColletotrichum gloeosporioides stored during 20 days at 12°C. HW‐Ca treated fruits showed higher ascorbic acid, phenolics content, and antioxidant activity than untreated and HW and Ca treated fruit, which correlated with the contents of ascorbic acid, phenolics, and β‐cryptoxanthin. Also, HW‐Ca treatment resulted in fruits showing lowe r decay area, greater firmness and color retention. The r...
Source: Journal of Food Biochemistry - October 24, 2018 Category: Food Science Authors: Lidia Elena Ay ón‐Reyna,
Francisco Delgado‐Vargas,
Carlos Andrés Soltero‐Sánchez,
Gabriela López‐Angulo,
Martha Edith López‐López,
Jordi Gerardo López‐Velázquez,
Jesús Ricardo Parra‐Unda,
Misael Odín Vega‐García Tags: FULL ARTICLE Source Type: research
A catecholase from Laccaria laccata a wild edible mushroom and its catalytic efficiency in organic media
In this study, catecholase was purified fromLaccaria laccata by affinity chromatography and the enzyme activity was investigated in organic media. Among the tested substrates, the highest catecholase activity was observed in 4 ‐MC, DHPPA, and L‐DOPA. A single band around 58.1 kDa was observed on SDS‐PAGE of the purified enzyme. Km values were calculated as 0.25, 0.40, and 0.83 mM for 4‐MC, DHPPA, and L‐DOPA, respectively. The highestVmax value was calculated as 2500 U/mg protein for 4 ‐MC. The inhibitors used in this study showed mixed type inhibition kinetics for all tested substrates. Ascorbic acid was foun...
Source: Journal of Food Biochemistry - October 24, 2018 Category: Food Science Authors: Yakup Kolcuo ğlu,
Ilksen Kuyumcu,
Ahmet Colak Tags: FULL ARTICLE Source Type: research
Hypercholesterolemia, angiotensin converting enzyme and ecto ‐enzymes of purinergic system: Ameliorative properties of caffeic and chlorogenic acid in hypercholesterolemic rats
AbstractThe aim of this study was to investigate the modulatory properties of caffeic acid (CA) and chlorogenic acid (CH) on the activity of angiotensin converting enzyme (ACE), purinergic enzyme (PE), and lipid lowering properties in hypercholesterolemic rats. CA and CH (10 or 15 mg/BW) were administered on hypercholesterolemic rats for 21 days while simvastatin (0.2 mg/BW) was used as a reference drug. Effects of CA and CH on PE, adenosine deaminase (ADA), ACE, atherogenic index as well as antioxidant status were determined. CA and CH positively modulated activity of e‐ NTPDase, reduced activity of the ADA and ACE,...
Source: Journal of Food Biochemistry - October 24, 2018 Category: Food Science Authors: Odunayo Michael Agunloye,
Ganiyu Oboh Tags: FULL ARTICLE Source Type: research
Valorization of spent shiitake substrate for recovery of antitumor fungal sterols by ultrasound ‐assisted extraction
This study aimed to investigate ultrasound ‐assisted extraction (UAE) of fungal sterols from spent shiitake (Lentinula edodes) substrate (SSS) by response surface methodology. The results showed that three variables, including ultrasonic power, extraction time, and liquid –solid ratio, had considerable influence on the extraction efficiency of total fungal sterols with liquid–solid ratio being highly significant for the process. The optimal UAE conditions included liquid–solid ratio 22 mL/g, ultrasonic power 432 W, and extraction time 16 min for the maximum recovery of fungal sterols (151.62 mg ergosterol equiv...
Source: Journal of Food Biochemistry - October 24, 2018 Category: Food Science Authors: Qi Wang,
Junxia Cheng,
Lixia Wang,
Shuaishuai Yan,
Ruiqin Wang,
Haisheng Zhang,
Hongjun Shao,
Xingbin Yang Tags: FULL ARTICLE Source Type: research
