In vivo wound healing potential and HPLC ‐PDA‐ESI‐MS profiling of Zizyphus lotus L. (Desf.) leaves methanol extract

This study was performed to evaluate the wound healing potential and antioxidant effect of methanol extract ofZizyphus lotus leaves (MZL), and to identify its phenolic profile by HPLC ‐PDA‐ESI‐MS. Wound healing activity was assessed in vivo using excision model. Biochemical and histopathological analyses were conducted. In vitro antioxidant potential of MZL was tested using 2,2‐diphenyl‐1‐picrylhydrazyl and β‐carotene bleaching methods. HPLC‐PDA‐ESI‐MS was us ed for the phenolic profiling. Animals treated with MZL ointment showed a significant reduction in epithelialization time and high contraction rate compared to controls. Proteins, hydroxyproline, and hexosamines contents of ointment‐treated animals were higher than controls. Marked cross‐linking of collagen fibers and lack of inflammatory cells were observed in the granulation tissue of the ointment‐treated animals. Chemical profiling of MZL revealed that quercetin‐3‐O‐rutinoside and catechin are the major constituents. Phenolic compounds of the methanol extract ofZizyphus lotus leaves may be engaged in the acceleration of the healing process activity.Practical applicationIn Mediterranean region, like Algeria,Zizyphus lotus is used in nutrition, health, and cosmetics. Several parts of this plant are employed to treat urinary troubles, diabetes, skin infections, fever, diarrhea, insomnia, bronchitis, and hypoglycemia. There is no scientific paper investigating the phenolic composition ofZ....
Source: Journal of Food Biochemistry - Category: Food Science Authors: Tags: FULL ARTICLE Source Type: research