TOX expression decreases with progression of colorectal cancers and is associated with CD4 T-cell density and Fusobacterium nucleatum infection
Fusobacterium nucleatum (F. nucleatum) in the tumor microenvironment plays an important role in the development of colorectal cancer (CRC). The underlying mechanism of action, however, remains to be elucidated. We evaluated the relation of F. nucleatum amount to thymocyte selection-associated high-mobility group box (TOX) protein expression and CD4+ T cell density in 138 human colorectal tissues. TOX expression and CD4+ T cell density in F. nucleatum negative tissues were significantly higher compared to those in F. (Source: Human Pathology)
Source: Human Pathology - May 21, 2018 Category: Pathology Authors: Ting Chen, Qing Li, Xiaoyan Zhang, Ran Long, Yaxin Wu, Jiao Wu, Xiangsheng Fu Tags: Original Contributions Source Type: research
Pathological significance and prognostic roles of densities of CD57+ cells, CD68+ cells, and mast cells, and their ratios in clear cell renal cell carcinoma
In this study, the clinical significance of densities of CD57+ cells, CD68+ cells, and mast cells, and their ratios were investigated in patients with clear cell RCC. The densities of CD57+, CD68+, and mast cells were evaluated by immunohistochemical techniques in 179 patients. (Source: Human Pathology)
Source: Human Pathology - May 19, 2018 Category: Pathology Authors: Hiromi Nakanishi, Yasuyoshi Miyata, Yasushi Mochizuki, Takuji Yasuda, Yuichiro Nakamura, Kyohei Araki, Yuji Sagara, Tomohiro Matsuo, Kojiro Ohba, Hideki Sakai Tags: Original Contributions Source Type: research
Clinicopathological characteristics of thyroid transcription factor 1-negative small cell lung cancers
This study aimed to elucidate the characteristics of TTF-1-negative SCLC. We studied the associations between the expression of TTF-1 and the clinicopathological factors associated with SCLC, including survival and expression of neuroendocrine markers (synaptophysin, chromogranin A, and CD56), neuroendocrine cell-specific transcription factors (achaete-scute homolog like 1 (ASCL1), BRN2), a proliferation marker (Ki-67 labeling index), and an oncogene (Nuclear Factor 1B (NF1B)). (Source: Human Pathology)
Source: Human Pathology - May 19, 2018 Category: Pathology Authors: Yuko Iida, Shinobu Masuda, Yoko Nakanishi, Tetsuo Shimizu, Haruna Nishimaki, Mai Takahashi, Mari Hikichi, Shuichiro Maruoka, Yasuhiro Gon, Noriaki Takahashi, Shu Hashimoto Tags: Original contribution Source Type: research
Budding and tumor infiltrating lymphocytes – combination of both parameters predicts survival in colorectal cancer and leads to new prognostic subgroups
Tumor budding is an independent prognostic factor in colorectal cancer (CRC) and has recently been well defined by the International Concensus Conference on Tumor Budding (ITBCC). Tumor infiltrating lymphocytes (TILs) are also an issue in different human cancers and correlate with prognosis in CRC.Here we evaluate the combination of budding and TILs in CRC with regard to prognosis.Hematoxylin and Eosin (H&E) stained slides of 501 CRC patients, diagnosed between 2005 and 2010 were reevaluated for tumor budding according to the ITBCC-criteria. (Source: Human Pathology)
Source: Human Pathology - May 19, 2018 Category: Pathology Authors: Corinna Lang-Schwarz, Balint Melcher, Franziska Haumaier, Klaus Lang-Schwarz, Thomas Rupprecht, Michael Vieth, William Sterlacci Tags: Original contribution Source Type: research
Clinicopathological analysis of immunohistochemical expression of retinoic acid-related orphan receptor- γt in peripheral T-cell lymphoma, not otherwise specified
Peripheral T-cell lymphoma, not otherwise specified (PTCL-NOS) is cytologically and phenotypically heterogeneous. Retinoic acid-related orphan receptor- γt (RORγt) is a transcription factor that regulates the differentiation of naïve CD4+ helper T-cells to Th17 cells. In the present study, we immunohistochemically confirmed the expression of RORγt in PTCL-NOS. Pathological and clinical investigations were performed for 170 cases of PTCL-NOS. ROR γt-positive cases accounted for 17.6% (30/170) of the total cases and they showed a significantly higher frequency of CD8 positivity (P=.033), lower counts of white blood cell...
Source: Human Pathology - May 15, 2018 Category: Pathology Authors: Eriko Yanagida, Hiroaki Miyoshi, Keisuke Kawamoto, Kazutaka Nakashima, Kotaro Matsuda, Kyohei Yamada, Reiji Muto, Koji Nagafuji, Masao Seto, Koichi Ohshima Tags: Original contribution Source Type: research
BAP1 loss is unusual in well-differentiated papillary mesothelioma and may predict development of malignant mesothelioma
Literature on BRCA1-associated protein 1 (BAP1) expression status in well-differentiated papillary mesothelioma (WDPM) is limited. In the present study, we examined the prevalence of BAP1 loss in WDPM by immunohistochemistry with clinical correlation, along with CDKN2A deletion status by fluorescence in situ hybridization (FISH). Eight patients diagnosed as WDPM were identified from the surgical pathology file. Adenomatoid tumors (n=8) and malignant mesothelioma (MM) (n=39) were included for comparison. (Source: Human Pathology)
Source: Human Pathology - May 12, 2018 Category: Pathology Authors: Hee Eun Lee, Julian R. Molina, William R. Sukov, Anja C. Roden, Eunhee S. Yi Tags: Original contribution Source Type: research
TPL2 expression is correlated with distant metastasis and poor prognosis in colorectal cancer
Tumor progression locus 2 (TPL2) is a mitogen-activated protein kinase (MAPK) kinase kinase (MAP3K). The present study aimed to elucidate the clinicopathological significance and prognostic role of TPL2 expression in colorectal cancer (CRC) through immunohistochemistry. In the present study, the correlations between TPL2 expression and clinicopathological parameters, including survival rate, were investigated using 262 archival paraffin-embedded CRC tissue samples. In addition, the correlation between TPL2 expression and tumor-infiltrating lymphocytes was evaluated using immunoscore. (Source: Human Pathology)
Source: Human Pathology - May 12, 2018 Category: Pathology Authors: Jung-Soo Pyo, Mee Ja Park, Chang-Nam Kim Tags: Original contribution Source Type: research
Prognostic factors in primary anorectal melanoma: a Clinicopathologic study of 60 cases in China
To investigate the clinicopathologic features and prognostic factors of primary anorectal melanoma, we described the clinical presentations, histopathology, and prognosis of 60 cases in China. Patients' age ranged from 17 to 86years with a female-to-male ratio of 2.33:1. The prevalent anatomic site of tumor was anorectum. The mean tumor thickness was 11.5mm and mean tumor size was 2.9cm. Abdominoperineal resection was performed on 38 (63.3%) patients, while wide local excision on 22 (36.7%) patients. (Source: Human Pathology)
Source: Human Pathology - May 12, 2018 Category: Pathology Authors: Min Ren, Yawen Lu, Jiaojie Lv, Xuxia Shen, Jincheng Kong, Bo Dai, Yunyi Kong Tags: Original Contributions Source Type: research
Small cell carcinoma of the urinary bladder: a Clinicopathologic and Immunohistochemical analysis of 81 cases
Small cell carcinoma (SmCC) of the bladder is a rare disease. We retrospectively studied a large series of bladder SmCC from a single institution. The patients included 69 men and 12 women with a mean age of 68years. Most bladder SmCC were presented at advanced stage with tumors invading the muscularis propria and beyond (n=77). SmCC was pure in 27 cases and mixed with other histologic types in 54 cases, including urothelial carcinoma (UC) (n=32), UC in situ (n=26), glandular (n=14), micropapillary (n=4), sarcomatoid (n=4), and squamous (n=3), and plasmacytoid (n=1) features. (Source: Human Pathology)
Source: Human Pathology - May 12, 2018 Category: Pathology Authors: Gang Wang, Li Xiao, Miao Zhang, Ashish M. Kamat, Arlene Siefker-Radtke, Colin P. Dinney, Bogdan Czerniak, Charles C. Guo Tags: Original Contributions Source Type: research
New insights into hypoxia-related mechanisms involved in different microvascular patterns of bronchopulmonary carcinoids and poorly differentiated neuroendocrine carcinomas. Role of ribonuclease T2 (RNASET2) and HIF-1 alpha
In this study, we investigated RNASET2 expression in well-differentiated and poorly differentiated neuroendocrine neoplasms of the lung (Lu-NENs), which are known to show clear-cut differences in morphology, biology and clinical behavior. (Source: Human Pathology)
Source: Human Pathology - May 12, 2018 Category: Pathology Authors: Silvia Uccella, Stefano La Rosa, Debora Scaldaferri, Laura Monti, Roberta Maragliano, Elisa Sorrenti, Marzia Gariboldi, Roberto Taramelli, Fausto Sessa, Francesco Acquati Tags: Original Contributions Source Type: research
Hepatic micrometastases are associated with poor prognosis in patients with liver metastases from neuroendocrine tumors of the digestive tract
Pathologic examination of hepatic metastasectomies from patients with metastatic small intestinal or pancreatic neuroendocrine tumor frequently reveals micrometastases undetectable by radiologic or macroscopic gross examination. This finding raises the possibility that undetectable micrometastases remain in these patients after metastasectomy. Here we examined liver resections for micrometastases and assessed their impact on prognosis. Hepatic metastasectomies from 65 patients with neuroendocrine tumor of the small intestine (N=43) or pancreas (N=22) were reviewed for the presence of micrometastases, which were defined as ...
Source: Human Pathology - May 12, 2018 Category: Pathology Authors: William E. Gibson, Raul S. Gonzalez, Justin M.M. Cates, Eric Liu, Chanjuan Shi Tags: Original contribution Source Type: research
Role of HNF1 β in the differential diagnosis from other germ cell tumors
Identification of the yolk sac tumor (YST) component in germ cell tumors (GCT) may prove challenging, and highly sensitive and specific immunohistochemical markers are still lacking. Preliminary data from the literature suggest that HNF1 β may represent a sensitive marker of YST. The specificity of HNF1β has not been addressed in GCT. A cohort of 49 YST specimens from 45 patients was designed, occurring either as pure tumors, or as a component of a mixed GCT. Immunohistochemistry was conducted on whole tumor sections using HNF1β. (Source: Human Pathology)
Source: Human Pathology - May 10, 2018 Category: Pathology Authors: Anne-Laure Rougemont, Jean-Christophe Tille Tags: Original Contributions Source Type: research
Evaluating agreement, histological features, and relevance of separating pleomorphic and florid lobular carcinoma in situ subtypes
Morphological variants of lobular carcinoma in situ (LCIS) include classical (CLCIS), pleomorphic (PLCIS) and florid type (FLCIS). Treatment guidelines suggest managing PLCIS and FLCIS like ductal carcinoma in situ (DCIS); therefore accurate identification of LCIS subtypes is critical. However, the significance of separating PLCIS from FLCIS is not clear. Also, interobserver agreement in identifying LCIS subtypes, using contemporary criteria, is not known. We aimed to evaluate interobserver agreement amongst breast pathologists in diagnosing LCIS subtypes and use the agreement data to justify LCIS classification for manage...
Source: Human Pathology - May 9, 2018 Category: Pathology Authors: Kamaljeet Singh, Cherie Paquette, Elizabeth T. Kalife, Yihong Wang, Shamlal Mangray, M. Ruhul Quddus, Margaret M. Steinhoff Tags: Original contribution Source Type: research
Determination of the mutational landscape in Taiwanese patients with papillary thyroid cancer by whole-exome sequencing
Among women in Taiwan, thyroid cancer is the fifth most common malignant neoplasia. However, genomic profiling of papillary thyroid cancer (PTC) cases from Taiwan has not been attempted previously. We used whole-exome sequencing to identify mutations in a cohort of 19 PTC patients. Sequencing was performed using the Illumina system; Sanger sequencing was used to validate all identified mutations. We identified new somatic mutations in APC, DICER1, LRRC8D and NDRG1. We also found somatic mutations in ARID5A, CREB3L2, MDM4, PPP2R5A and TFPT; mutations in these genes had been found previously in other tumors, but had not been...
Source: Human Pathology - May 9, 2018 Category: Pathology Authors: Chun-Chi Chang, Ya-Sian Chang, Hsi-Yuan Huang, Kun-Tu Yeh, Ta-Chih Liu, Jan-Gowth Chang Tags: Original contribution Source Type: research
Overexpression of MCM6 predicts poor survival in patients with glioma
Minichromosome maintenance proteins (MCMs) play an essential role in DNA replication and other cellular activities. However, their expression levels and clinical value in glioma are unclear. In the present study, we analyzed the relationship between MCM mRNA expression and clinical parameters in 325 gliomas, and found that MCM6 presented high expression and was associated with poor survival. Immunohistochemistry analysis of an independent dataset of 423 glioma tissues confirmed the overexpression of MCM6 protein, especially in glioblastomas (GBMs) with shorter overall survival (OS). (Source: Human Pathology)
Source: Human Pathology - May 9, 2018 Category: Pathology Authors: Hong-Qing Cai, Zhi-Jian Cheng, Hai-Peng Zhang, Peng-Fei Wang, Yu Zhang, Jia-Jie Hao, Ming-Rong Wang, Jing-Hai Wan Source Type: research
