Issue Information (TOC and EDBD)
(Source: genesis)
Source: genesis - June 17, 2016 Category: Genetics & Stem Cells Tags: Issue Information Source Type: research
Cover Image
(Source: genesis)
Source: genesis - June 17, 2016 Category: Genetics & Stem Cells Tags: Cover Image Source Type: research
Cre ‐dependent DREADD (Designer Receptors Exclusively Activated by Designer Drugs) mice
Summary
DREADDs, designer receptors exclusively activated by designer drugs, are engineered G protein‐coupled receptors (GPCR) which can precisely control GPCR signaling pathways (for example, Gq, Gs, and Gi). This chemogenetic technology for control of GPCR signaling has been successfully applied in a variety of in vivo studies, including in mice, to remotely control GPCR signaling, for example, in neurons, glia cells, pancreatic β‐cells, or cancer cells. In order to fully explore the in vivo applications of the DREADD technology, we generated hM3Dq and hM4Di strains of mice which allow for Cre recombinase‐mediated...
Source: genesis - June 2, 2016 Category: Genetics & Stem Cells Authors: Hu Zhu, Dipendra K. Aryal, Reid H.J. Olsen, Daniel J. Urban, Amanda Swearingen, Stacy Forbes, Bryan L. Roth, Ute Hochgeschwender Tags: Technology Report Source Type: research
Cre‐dependent DREADD (Designer Receptors Exclusively Activated by Designer Drugs) mice
Summary
DREADDs, designer receptors exclusively activated by designer drugs, are engineered G protein‐coupled receptors (GPCR) which can precisely control GPCR signaling pathways (for example, Gq, Gs, and Gi). This chemogenetic technology for control of GPCR signaling has been successfully applied in a variety of in vivo studies, including in mice, to remotely control GPCR signaling, for example, in neurons, glia cells, pancreatic β‐cells, or cancer cells. In order to fully explore the in vivo applications of the DREADD technology, we generated hM3Dq and hM4Di strains of mice which allow for Cre recombinase‐mediated...
Source: genesis - June 2, 2016 Category: Genetics & Stem Cells Authors: Hu Zhu, Dipendra K. Aryal, Reid H.J. Olsen, Daniel J. Urban, Amanda Swearingen, Stacy Forbes, Bryan L. Roth, Ute Hochgeschwender Tags: Technology Report Source Type: research
A new gain‐of‐function mouse line to study the role of Wnt3a in development and disease
This article is protected by copyright. All rights reserved. (Source: genesis)
Source: genesis - May 31, 2016 Category: Genetics & Stem Cells Authors: Ravindra B. Chalamalasetty, Rieko Ajima, Robert Garriock, Mark Kennedy, Lino Tessarollo, Terry P. Yamaguchi Tags: Technology Report Source Type: research
Antennas of organ morphogenesis: The roles of cilia in vertebrate kidney development
This article is protected by copyright. All rights reserved. (Source: genesis)
Source: genesis - May 31, 2016 Category: Genetics & Stem Cells Authors: Amanda N. Marra, Yue Li, Rebecca A. Wingert Tags: Review Source Type: research
Generation and characterization of tamoxifen‐inducible Pax9‐CreER Knock‐In Mice using CrispR/Cas9
This article is protected by copyright. All rights reserved. (Source: genesis)
Source: genesis - May 31, 2016 Category: Genetics & Stem Cells Authors: Jifan Feng, Junjun Jing, Pedro A Sanchez‐Lara, Moiz S Bootwalla, Jonathan Buckley, Nancy Wu, Youzhen Yan, Yang Chai Tags: Technology Report Source Type: research
Zebrafish lines expressing UAS‐driven red probes for monitoring cytoskeletal dynamics
This article is protected by copyright. All rights reserved. (Source: genesis)
Source: genesis - May 31, 2016 Category: Genetics & Stem Cells Authors: Takamasa Mizoguchi, Koichi Kawakami, Motoyuki Itoh Tags: Technology Report Source Type: research
Generation of an inducible fibroblast cell line for studying direct cardiac reprogramming
This article is protected by copyright. All rights reserved. (Source: genesis)
Source: genesis - May 18, 2016 Category: Genetics & Stem Cells Authors: Haley Ruth Vaseghi, Chaoying Yin, Yang Zhou, Li Wang, Jiandong Liu, Li Qian Tags: Technology Report Source Type: research
Cre dependent DREADD (designer receptors exclusively activated by designer drugs) mice
This article is protected by copyright. All rights reserved. (Source: genesis)
Source: genesis - May 18, 2016 Category: Genetics & Stem Cells Authors: Hu Zhu, Dipendra K. Aryal, Reid H.J. Olsen, Daniel J. Urban, Amanda Swearingen, Stacy Forbes, Bryan L. Roth, Ute Hochgeschwender Tags: Technology Report Source Type: research
Issue Information (TOC and EDBD)
(Source: genesis)
Source: genesis - May 17, 2016 Category: Genetics & Stem Cells Tags: Issue Information Source Type: research
Cover Image
(Source: genesis)
Source: genesis - May 17, 2016 Category: Genetics & Stem Cells Tags: Cover Image Source Type: research
Exclusive transmission of the embryonic stem cell‐derived genome through the mouse germline
Summary
Gene targeting in embryonic stem (ES) cells remains best practice for introducing complex mutations into the mouse germline. One aspect in this multistep process that has not been streamlined with regard to the logistics and ethics of mouse breeding is the efficiency of germline transmission: the transmission of the ES cell‐derived genome through the germline of chimeras to their offspring. A method whereby male chimeras transmit exclusively the genome of the injected ES cells to their offspring has been developed. The new technology, referred to as goGermline, entails injecting ES cells into blastocysts produced...
Source: genesis - May 17, 2016 Category: Genetics & Stem Cells Authors: Frank Koentgen, Jiangwei Lin, Markella Katidou, Isabelle Chang, Mona Khan, Jacqui Watts, Peter Mombaerts Tags: Research Article Source Type: research
Germline recombination in a novel Cre transgenic line, Prl3b1 ‐Cre mouse
In conclusion, our results suggest that the Prl3b1‐cre mice line provides a unique resource to understand testicular germ‐cell development. genesis 54:389–397, 2016. © 2016 Wiley Periodicals, Inc. (Source: genesis)
Source: genesis - May 12, 2016 Category: Genetics & Stem Cells Authors: Al ‐Sayed Al‐Soudy, Tsuyoshi Nakanishi, Seiya Mizuno, Yoshikazu Hasegawa, Hossam H. Shawki, Megumi C. Katoh, Walaa A. Basha, Abdelaziz E. Ibrahim, Hany A. El‐Shemy, Hiroyoshi Iseki, Atsushi Yoshiki, Youhei Hiromori, Hisamitsu Nagase, Satoru Takahash Tags: Technology Report Source Type: research
The untapped potential of the GENSAT mice—A valuable resource for developmental biology
Summary
Gene Expression Nervous System Atlas (GENSAT) transgenic mice express EGFP, tdTomato, or Cre recombinase in a wide range of cell types. The mice and the bacterial artificial chromosome transgenes are available from repositories (MMRRC or CHORI), thereby making these resources readily available to the research community. This resource of 1,386 transgenic lines was developed and validated for neuroscience research. However, GENSAT mice have many potential applications in other contexts including studies of development outside of the CNS. The cell type‐specific expression of fluorescent proteins in these mice has be...
Source: genesis - May 2, 2016 Category: Genetics & Stem Cells Authors: Brian G. Condie Tags: Review Source Type: research
