Inhibition of P ‐TEFb disrupts global transcription, oocyte maturation, and embryo development in the mouse
In this study, using immunocytochemical analyses, we find that the P‐TEFb components, Cyclin T1, CDK9, and its T‐loop phosphorylated form, are localized to nuclear speckles, as well as in nucleoli in mouse germinal vesicle oocytes. Moreover, using fluorescence in situ hybridization, we show that in absence of CDK9 activity, nucleolar integration, as well as production of 28S rRNA is impaired in oocytes and embryos. We also present evidence indicating that P‐TEFb kinase activity is essential for completion of mouse oocyte maturation and embryo development. Treatment with CDK9 inhibitor, flavopiridol resulted in metaph...
Source: genesis - August 17, 2016 Category: Genetics & Stem Cells Authors: Reza K. Oqani, Tao Lin, Jae Eun Lee, So Yeon Kim, Soo Jin Sa, Je Seok Woo, Dong Il Jin Tags: Research Article Source Type: research
Pou4f2 ‐GFP knock‐in mouse line: A model for studying retinal ganglion cell development
Summary
Pou4f2 acts as a key node in the comprehensive and step‐wise gene regulatory network (GRN) and regulates the development of retinal ganglion cells (RGCs). Accordingly, deletion of Pou4f2 results in RGC axon defects and apoptosis. To investigate the GRN involved in RGC regeneration, we generated a mouse line with a POU4F2‐green fluorescent protein (GFP) fusion protein expressed in RGCs. Co‐localization of POU4F2 and GFP in the retina and brain of Pou4f2‐GFP/+ heterozygote mice was confirmed using immunofluorescence analysis. Compared with those in wild‐type mice, the expression patterns of POU4F2 and POU4F...
Source: genesis - August 16, 2016 Category: Genetics & Stem Cells Authors: Dongwang Zheng, Xiaoyan Yang, Donglai Sheng, Dongliang Yu, Guoqing Liang, Luming Guo, Mei Xu, Xu Hu, Daqiang He, Yang Yang, Yuying Wang Tags: Technology Report Source Type: research
Reappraisal of VAChT ‐Cre: Preference in Slow Motor Neurons Innervating Type I or IIa Muscle Fibers
This article is protected by copyright. All rights reserved. (Source: genesis)
Source: genesis - July 31, 2016 Category: Genetics & Stem Cells Authors: Hidemi Misawa, Daijiro Inomata, Miseri Kikuchi, Sae Maruyama, Yasuhiro Moriwaki, Takashi Okuda, Nobuyuki Nukina, Tomoyuki Yamanaka Tags: Technology Report Source Type: research
Role of Tau, a microtubule associated protein, in Drosophila photoreceptor morphogenesis
This article is protected by copyright. All rights reserved. (Source: genesis)
Source: genesis - July 31, 2016 Category: Genetics & Stem Cells Authors: Sang ‐Chul Nam Tags: Research Article Source Type: research
Issue Information (TOC and EDBD)
(Source: genesis)
Source: genesis - July 19, 2016 Category: Genetics & Stem Cells Tags: Issue Information Source Type: research
Cover Image
(Source: genesis)
Source: genesis - July 19, 2016 Category: Genetics & Stem Cells Tags: Cover Image Source Type: research
A knock ‐in allele of En1 expressing dre recombinase
This article is a US Government work and is in the public domain in the USA. (Source: genesis)
Source: genesis - July 8, 2016 Category: Genetics & Stem Cells Authors: Nicholas W. Plummer, Jacqueline de Marchena, Patricia Jensen Tags: Technology Report Source Type: research
A compact unc45b ‐promoter drives muscle‐specific expression in zebrafish and mouse
Summary: Gene therapeutic approaches to cure genetic diseases require tools to express the rescuing gene exclusively within the affected tissues. Viruses are often chosen as gene transfer vehicles but they have limited capacity for genetic information to be carried and transduced. In addition, to avoid off‐target effects the therapeutic gene should be driven by a tissue‐specific promoter in order to ensure expression in the target organs, tissues, or cell populations. The larger the promoter, the less space will be left for the respective gene. Thus, there is a need for small but tissue‐specific promoters. Here, we d...
Source: genesis - July 8, 2016 Category: Genetics & Stem Cells Authors: Steven Rudeck, Christelle Etard, Muzamil M. Khan, Wolfgang Rottbauer, R üdiger Rudolf, Uwe Strähle, Steffen Just Tags: Letter Source Type: research
Generation and characterization of tamoxifen ‐inducible Pax9‐CreER Knock‐In Mice using CrispR/Cas9
This article is protected by copyright. All rights reserved. (Source: genesis)
Source: genesis - July 5, 2016 Category: Genetics & Stem Cells Authors: Jifan Feng, Junjun Jing, Pedro A Sanchez ‐Lara, Moiz S Bootwalla, Jonathan Buckley, Nancy Wu, Youzhen Yan, Yang Chai Tags: Technology Report Source Type: research
Broad ‐complex, Tramtrack, and Bric‐à‐brac (BTB) Proteins: Critical Regulators of Development
This article is protected by copyright. All rights reserved. (Source: genesis)
Source: genesis - June 30, 2016 Category: Genetics & Stem Cells Authors: Edwin Chaharbakhshi, Jennifer C. Jemc Tags: Review Source Type: research
Transcriptional regulation of the proto ‐oncogene Zfp521 by SPI1 (PU.1) and HOXC13
This article is protected by copyright. All rights reserved. (Source: genesis)
Source: genesis - June 30, 2016 Category: Genetics & Stem Cells Authors: Ming Yu, Salma Al ‐Dallal, Latifa Al‐Haj, Shiraj Panjwani, Akina S. McCartney, Sarah M. Edwards, Pooja Manjunath, Catherine Walker, Alexander Awgulewitsch, Kathryn E. Hentges Tags: Research Article Source Type: research
Inhibition of P ‐TEFb Disrupts Global Transcription, Oocyte Maturation and Embryo Development in the Mouse
This article is protected by copyright. All rights reserved. (Source: genesis)
Source: genesis - June 30, 2016 Category: Genetics & Stem Cells Authors: Reza K. Oqani, Tao Lin, Jae Eun Lee, So Yeon Kim, Soo Jin Sa, Je Seok Woo, Dong Il Jin Tags: Research Article Source Type: research
A new gain ‐of‐function mouse line to study the role of Wnt3a in development and disease
This article is protected by copyright. All rights reserved. (Source: genesis)
Source: genesis - June 30, 2016 Category: Genetics & Stem Cells Authors: Ravindra B. Chalamalasetty, Rieko Ajima, Robert Garriock, Mark Kennedy, Lino Tessarollo, Terry P. Yamaguchi Tags: Technology Report Source Type: research
Zebrafish lines expressing UAS ‐driven red probes for monitoring cytoskeletal dynamics
This article is protected by copyright. All rights reserved. (Source: genesis)
Source: genesis - June 24, 2016 Category: Genetics & Stem Cells Authors: Takamasa Mizoguchi, Koichi Kawakami, Motoyuki Itoh Tags: Technology Report Source Type: research
Fate mapping of Trps1 daughter cells during cardiac development using novel Trps1 ‐Cre mice
Summary
Tricho‐rhino‐phalangeal syndrome (TRPS) is a rare congenital disorder that is characterized by abnormal hair growth and skeletal deformities. These result in sparse hair, short stature, and early onset of joint problems. Recent reports have shown that a relatively high proportion of patients with TRPS exhibit a broad range of congenital heart defects. To determine the regulation of Trps1 transcription in vivo, we generated novel transgenic mice, which expressed Cre recombinase under the murine Trps1 proximal promoter sequence (Trps1‐Cre). We crossed these mice with Cre reporter mice to identify Trps1 daughter...
Source: genesis - June 19, 2016 Category: Genetics & Stem Cells Authors: Ahmed G. Nomir, Yuto Takeuchi, Junji Fujikawa, Ashraf A. El Sharaby, Satoshi Wakisaka, Makoto Abe Tags: Research Article Source Type: research
