Sensitive detection of Porphyromonas gingivalis based on magnetic capture and upconversion fluorescent identification with multifunctional nanospheres
A specific and sensitive detection system was designed to detect Porphyromonas gingivalis, a major periodontal pathogen, in mixed bacterial fluids. This new detection system was based on the use of fluorescent and magnetic encoding nanospheres that were conjugated with monoclonal antibodies specific to P. gingivalis, thus enabling rapid detection of the target bacterium. This strategy simplifies the detection process and improves the sensitivity compared with conventional methods, with a detection limit of approximately 10 colony‐forming units (CFU) ml−1. This new method shows strong anti‐interference ability and exc...
Source: European Journal of Oral Sciences - May 31, 2016 Category: Dentistry Authors: Wei Qin, Bin Zheng, Yuan Yuan, Meng Li, Yang Bai, Jin Chang, Hanjie Wang, Yonglan Wang Tags: Original Article Source Type: research
Influence of duration of phosphoric acid pre‐etching on bond durability of universal adhesives and surface free‐energy characteristics of enamel
The purpose of this study was to evaluate the influence of duration of phosphoric acid pre‐etching on the bond durability of universal adhesives and the surface free‐energy characteristics of enamel. Three universal adhesives and extracted human molars were used. Two no‐pre‐etching groups were prepared: ground enamel; and enamel after ultrasonic cleaning with distilled water for 30 s to remove the smear layer. Four pre‐etching groups were prepared: enamel pre‐etched with phosphoric acid for 3, 5, 10, and 15 s. Shear bond strength (SBS) values of universal adhesive after no thermal cycling and after 30,000 or 60...
Source: European Journal of Oral Sciences - May 31, 2016 Category: Dentistry Authors: Akimasa Tsujimoto, Wayne W. Barkmeier, Toshiki Takamizawa, Hidehiko Watanabe, William W. Johnson, Mark A. Latta, Masashi Miyazaki Tags: Original Article Source Type: research
Prevalence and patterns of permanent tooth agenesis in individuals with Down syndrome: a meta‐analysis
In conclusion, individuals with Down syndrome display high prevalence rates and severity of agenesis of permanent teeth. Proper and timely diagnosis of missing permanent teeth is thus necessary, to allow a more comprehensive long‐term treatment plan and a more favorable prognosis in these individuals. (Source: European Journal of Oral Sciences)
Source: European Journal of Oral Sciences - May 31, 2016 Category: Dentistry Authors: Pinelopi K. Palaska, Gregory S. Antonarakis Tags: Original Article Source Type: research
Expression of TET2 enzyme indicates enhanced epigenetic modification of cells in periodontitis
DNA methylation is an important epigenetic mechanism involved in the regulation of gene expression, and a reduction in DNA methylation influences cell‐cycle progression and cell differentiation in inflammatory cells. The aim of the present study was to analyze the DNA‐methylation pattern at local and global/systemic levels in patients with periodontitis and gingivitis. Twenty‐one subjects with generalized, severe periodontitis and 17 subjects with gingival inflammation but no attachment loss were recruited. Gingival biopsies and peripheral blood samples were collected and prepared for immunohistochemical analysis of ...
Source: European Journal of Oral Sciences - May 31, 2016 Category: Dentistry Authors: Lena Larsson, Sara Thorbert‐Mros, Aaron Lopez‐Lago, Josephine Kalm, Asal Shikhan, Tord Berglundh Tags: Original Article Source Type: research
Interaction between intra‐oral cinnamaldehyde and nicotine assessed by psychophysical and physiological responses
This study investigated whether cinnamaldehyde modulates nicotine‐induced psychophysical and physiological responses in oral tissues. Healthy non‐smokers (n = 22) received, in a randomized, double‐blind, crossover design, three different gums containing 4 mg of nicotine, 20 mg of cinnamaldehyde, or a combination thereof. Assessments of orofacial temperature and blood flow, blood pressure, heart rate, taste experience, and intra‐oral pain/irritation area and intensity were performed before, during, and after a 10‐min chewing regime. Cinnamaldehyde increased the temperature of the tongue and blood flow of the lip, ...
Source: European Journal of Oral Sciences - May 31, 2016 Category: Dentistry Authors: Tanja K. Jensen, Michelle V. Andersen, Kent A. Nielsen, Lars Arendt‐Nielsen, Shellie A. Boudreau Tags: Original Article Source Type: research
