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Cytometry Part B: Clinical Cytometry, Ahead of Print. (Source: Cytometry Part B: Clinical Cytometry)
Source: Cytometry Part B: Clinical Cytometry - July 5, 2017 Category: Molecular Biology Source Type: research

Changes in tonsil B cell phenotypes and EBV receptor expression in children under 5 years old
Conclusions: We show that young children have a higher frequency of naïve tonsil B cells, and importantly that these cells express increased surface EBV receptor, suggesting that young children have a larger pool of cells that can be infected by the virus. This article is protected by copyright. All rights reserved. (Source: Cytometry Part B: Clinical Cytometry)
Source: Cytometry Part B: Clinical Cytometry - July 1, 2017 Category: Molecular Biology Authors: Eric M Wohlford, Paul C Baresel, Joel R Wilmore, Anthony J Mortelliti, Carrie B Coleman, Rosemary Rochford Tags: Original Article Source Type: research

PhenoGraph and viSNE Facilitate the Identification of Abnormal T ‐Cell Populations in Routine Clinical Flow Cytometric Data
Conclusions: PhenoGraph and viSNE may facilitate the identification of abnormal T‐cell populations in routine clinical flow cytometric data. This article is protected by copyright. All rights reserved. (Source: Cytometry Part B: Clinical Cytometry)
Source: Cytometry Part B: Clinical Cytometry - July 1, 2017 Category: Molecular Biology Authors: Joseph A. DiGiuseppe, Jolene L. Cardinali, William N. Rezuke, Dana Pe'er Tags: Original Article Source Type: research

Evaluation of biological fluid analysis using the Sysmex XN automatic hematology analyzer
Conclusions: The Except for AF, the Sysmex XN‐1000 results agree with those of the manual method, although to different degrees depending on the fluid type. This article is protected by copyright. All rights reserved. (Source: Cytometry Part B: Clinical Cytometry)
Source: Cytometry Part B: Clinical Cytometry - July 1, 2017 Category: Molecular Biology Authors: Vicente Aguadero, Ruth Cano, Eugenio Berlanga, Montserrat Torra Tags: Original Article Source Type: research

Multicolor flow cytometric analysis of TLR2 and TLR9 expression and function in NK cells from patients with ANCA ‐associated vasculitis
Conclusions: Our data suggest a role of infectious stimuli triggering NK cells in AAV pathogenesis. Poor detection of NK cells in affected tissues suggests a marginal involvement in local inflammatory responses. This article is protected by copyright. All rights reserved. (Source: Cytometry Part B: Clinical Cytometry)
Source: Cytometry Part B: Clinical Cytometry - July 1, 2017 Category: Molecular Biology Authors: Rossana Scrivo, Giovanna Peruzzi, Angelica Gattamelata, Catharina C. Gross, Raffaella Carletti, Cira Di Gioia, Jessica Brandt, Roberta Priori, Stefania Morrone, Angela Santoni, Guido Valesini Tags: Original Article Source Type: research

Clinical flow cytometry: It's not just about reaching a diagnosis
(Source: Cytometry Part B: Clinical Cytometry)
Source: Cytometry Part B: Clinical Cytometry - July 1, 2017 Category: Molecular Biology Authors: Fiona E. Craig Tags: Other Source Type: research

Flow cytometric analysis of viable bacteria in urine samples of febrile patients at the emergency department
Conclusion: FC counting of only viable bacteria can predict quickly and reliably positive and negative urine cultures in febrile patients admitted to the emergency department. It can help to improve the speed and accuracy of the diagnostic procedure at the emergency department. This article is protected by copyright. All rights reserved. (Source: Cytometry Part B: Clinical Cytometry)
Source: Cytometry Part B: Clinical Cytometry - July 1, 2017 Category: Molecular Biology Authors: Anne H. Tavenier, Foppie J. de Boer, Bijan Moshaver, Sjef J.C.M. van der Leur, Coen A. Stegeman, Paul H.P. Groeneveld Tags: Original Article Source Type: research

Influence of IL ‐6, IL‐33, and TNF‐α on Human Mast Cell Activation: Lessons from Single Cell Analysis by Flow Cytometry
Conclusions: In conclusion, IL‐33, and in a lesser extent IL‐6, prime individual MCs for subsequent IgE‐mediated activation. Moreover, this priming effect is synergistic. In contrast, none of the cytokines had a priming effect on MRGPRX2‐mediated activation of MCs. This article is protected by copyright. All rights reserved. (Source: Cytometry Part B: Clinical Cytometry)
Source: Cytometry Part B: Clinical Cytometry - July 1, 2017 Category: Molecular Biology Authors: Nathalie Cop, Didier G. Ebo, Chris H. Bridts, Jessy Elst, Margo M. Hagendorens, Christel Mertens, Margaretha A. Faber, Luc S. De Clerck, Vito Sabato Tags: Original Article Source Type: research

Flow cytometry vs optical microscopy in the evaluation of the genotoxic potential of xenobiotic compounds
Conclusions: This fact, in view of results supported by a high number of cells analyzed and obtained by an accurate and objective reading, with a considerable reduction of the analysis time, can support a future request for validation of the micronucleus analysis by FCM. This article is protected by copyright. All rights reserved. (Source: Cytometry Part B: Clinical Cytometry)
Source: Cytometry Part B: Clinical Cytometry - July 1, 2017 Category: Molecular Biology Authors: Monia Lenzi, Veronica Cocchi, Patrizia Hrelia Tags: Original Article Source Type: research

High ‐Dimensional Single Cell Mapping of Cerium Distribution in the Lung Immune Microenvironment of an Active Smoker
Conclusions: These results demonstrate the first high‐dimensional single cell characterization of environmental metal exposure associated with smoking, and offer a demonstration of the unique potential for applying mass cytometry to the field of environmental toxicology. This article is protected by copyright. All rights reserved. (Source: Cytometry Part B: Clinical Cytometry)
Source: Cytometry Part B: Clinical Cytometry - July 1, 2017 Category: Molecular Biology Authors: Adeeb H. Rahman, Yonit Lavin, Soma Kobayashi, Andrew Leader, Miriam Merad Tags: Brief Communications Source Type: research

Evaluation of Primary Mediastinal Large B cell Lymphoma by Flow Cytometry
CONCLUSIONS: FC can detect the NP of PMLBCL and a FC assay for CHL performed better than a B‐NHL assay in this regard. The neoplastic cells showed a B cell immunophenotype with over‐expression of CD40. The reactive infiltrate in PMLBCL shows unique features including a prominent CD8+ T cell infiltrate. This article is protected by copyright. All rights reserved. (Source: Cytometry Part B: Clinical Cytometry)
Source: Cytometry Part B: Clinical Cytometry - July 1, 2017 Category: Molecular Biology Authors: Sindhu Cherian, Jonathan R. Fromm Tags: Original Article Source Type: research

Single Cell Analysis of Human Tissues and Solid Tumors with Mass Cytometry
(Source: Cytometry Part B: Clinical Cytometry)
Source: Cytometry Part B: Clinical Cytometry - July 1, 2017 Category: Molecular Biology Authors: Nalin Leelatian, Deon B. Doxie, Allison R. Greenplate, Bret C. Mobley, Jonathan M. Lehman, Justine Sinnaeve, Rondi M. Kauffman, Jay A. Werkhaven, Akshitkumar M. Mistry, Kyle D. Weaver, Reid C. Thompson, Pierre P. Massion, Mary A. Hooks, Mark C. Kelley, Lo Tags: Other Source Type: research

The CLEC12A Receptor Marks Human Basophils; Potential Implications for Minimal Residual Disease Detection in Myeloid Malignancies
Conclusions: Identification and enumeration of basophils is of high relevance in diagnostic hematology and immunology. We here show that CLEC12A in a simple FCM assay consistently marks basophils. Importantly, since basophils are characterized by a CD45lowSSClow profile similar to the “blast‐gate” employed for the evaluation of hematological disorders, awareness of minor normal CLEC12A+ subpopulations is crucial when using CLEC12A as a minimal residual disease marker in myeloid malignancies. This article is protected by copyright. All rights reserved. (Source: Cytometry Part B: Clinical Cytometry)
Source: Cytometry Part B: Clinical Cytometry - July 1, 2017 Category: Molecular Biology Authors: Marie Toft ‐Petersen, Anne Stidsholt Roug, Trine Plesner, Lene Ebbesen, Gordon D. Brown, Line Nederby Tags: Original Article Source Type: research

Standardized high ‐sensitivity flow cytometry testing for paroxysmal nocturnal hemoglobinuria in children with acquired bone marrow failure disorders: A single center US study
Cytometry Part B: Clinical Cytometry, EarlyView. (Source: Cytometry Part B: Clinical Cytometry)
Source: Cytometry Part B: Clinical Cytometry - June 27, 2017 Category: Molecular Biology Authors: Rachel E.Donohue , Andrea N.Marcogliese , Ghadir S.Sasa , M. TarekElghetany , Alka A.Redkar , Alison A.Bertuch , Choladda V.Curry Source Type: research