The CLEC12A Receptor Marks Human Basophils; Potential Implications for Minimal Residual Disease Detection in Myeloid Malignancies

Conclusions: Identification and enumeration of basophils is of high relevance in diagnostic hematology and immunology. We here show that CLEC12A in a simple FCM assay consistently marks basophils. Importantly, since basophils are characterized by a CD45lowSSClow profile similar to the “blast‐gate” employed for the evaluation of hematological disorders, awareness of minor normal CLEC12A+ subpopulations is crucial when using CLEC12A as a minimal residual disease marker in myeloid malignancies. This article is protected by copyright. All rights reserved.

Source: Cytometry Part B: Clinical Cytometry - July 1, 2017 Category: Molecular Biology Authors: Marie Toft ‐Petersen, Anne Stidsholt Roug, Trine Plesner, Lene Ebbesen, Gordon D. Brown, Line Nederby Tags: Original Article Source Type: research