Clinical Microbiology and Infection This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Matrix ‐assisted laser desorption ionization time‐of‐flight mass spectrometry: revolutionizing clinical laboratory diagnosis of mould infections
Abstract The clinical diagnosis of mould infections currently involves complex species identification based on morphological criteria, which is often prone to error. Employing an extensive mould species reference spectral library (up to 2832 reference spectra, corresponding to 708 strains from 347 species), we assessed the extent to which matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) enhanced the accuracy of species identification. MALDI‐TOF MS data were validated against morphology‐based and DNA sequence‐based results with 262 clinical isolates collected over a...
Source: Clinical Microbiology and Infection - December 11, 2014 Category: Microbiology Authors: M. Gautier, S. Ranque, A. ‐C. Normand, P. Becker, A. Packeu, C. Cassagne, C. L'Ollivier, M. Hendrickx, R. Piarroux Tags: Original Article Source Type: research

Tigecycline therapy for carbapenem ‐resistant Klebsiella pneumoniae (CRKP) bacteriuria leads to tigecycline resistance
In conclusion, the use of tigecycline with CRKP bacteriuria is common, and is associated with the subsequent development of tigecycline resistance. (Source: Clinical Microbiology and Infection)
Source: Clinical Microbiology and Infection - December 11, 2014 Category: Microbiology Authors: D. Duin, E. D. Cober, S. S. Richter, F. Perez, M. Cline, K. S. Kaye, R. C. Kalayjian, R. A. Salata, S. R. Evans, V. G. Fowler, R. A. Bonomo Tags: Research Note Source Type: research

A simple, robust and rapid approach to detect carbapenemases in Gram ‐negative isolates by MALDI‐TOF mass spectrometry: validation with triple quadripole tandem mass spectrometry, microarray and PCR
Abstract Carbapenemases should be accurately and rapidly detected, given their possible epidemiological spread and their impact on treatment options. Here, we developed a simple, easy and rapid matrix‐assisted laser desorption ionization‐time of flight (MALDI‐TOF)‐based assay to detect carbapenemases and compared this innovative test with four other diagnostic approaches on 47 clinical isolates. Tandem mass spectrometry (MS‐MS) was also used to determine accurately the amount of antibiotic present in the supernatant after 1 h of incubation and both MALDI‐TOF and MS‐MS approaches exhibited a 100% sensitivity ...
Source: Clinical Microbiology and Infection - December 11, 2014 Category: Microbiology Authors: C. Vogne, G. Prod'hom, K. Jaton, L. A. Decosterd, G. Greub Tags: Original Article Source Type: research

Predicting pneumococcal community ‐acquired pneumonia in the emergency department
Abstract The aim of this study was to quantify the value of clinical predictors available in the emergency department (ED) in predicting Streptococcus pneumoniae as the cause of community‐acquired pneumonia (CAP). A prospective, observational, cohort study of patients with CAP presenting in the ED was performed. Pneumococcal aetiology of CAP was based on either bacteraemia, or S. pneumoniae being cultured from sputum, or urinary immunochromatographic assay positivity, or positivity of a novel serotype‐specific urinary antigen detection test. Multivariate logistic regression was used to identify independent predictors ...
Source: Clinical Microbiology and Infection - December 11, 2014 Category: Microbiology Authors: S. M. Huijts, W. G. Boersma, D. E. Grobbee, W. C. Gruber, K. U. Jansen, J. A. J. W. Kluytmans, B. A. F. Kuipers, F. Palmen, M. W. Pride, C. Webber, M. J. M. Bonten, Tags: Original Article Source Type: research

Detection of small amounts of human adenoviruses in stools: comparison of a new immuno real ‐time PCR assay with classical tools
Abstract The detection of low virus concentrations in biological matrices, especially stool samples, is facing significant limitations as far as common diagnostic methods (enzyme‐linked‐immunosorbent assay (ELISA) or quantitative real‐time PCR (qPCR)) are considered. Here the development of a new immuno real‐time PCR (iPCR) is described and its performance in the detection of human adenoviruses (HAdVs) in spiked stools is compared with those of ELISA and qPCR assays. For the iPCR, detection of the sandwich formed by the complexation of capture antibody‐antigen‐detection antibody was performed by qPCR thanks to ...
Source: Clinical Microbiology and Infection - December 11, 2014 Category: Microbiology Authors: S. Bonot, L. Ogorzaly, B. El Moualij, W. Zorzi, H. ‐M. Cauchie Tags: Original Article Source Type: research

Hepatitis C virus and interferon type III (interferon‐λ3/interleukin‐28B and interferon‐λ4): genetic basis of susceptibility to infection and response to antiviral treatment
Abstract There has been a significant increase in our understanding of the host genetic determinants of susceptibility to viral infections in recent years. Recently, two single‐nucleotide polymorphisms (SNPs), rs12979860 T/C and rs8099917 T/G, upstream of the interleukin (IL)‐28B/interferon (IFN)‐λ3 gene have been clearly associated with spontaneous and treatment‐induced viral clearance in hepatitis C virus (HCV) infection. Because of their power in predicting the response to IFN/ribavirin therapy, the above SNPs have been used as a diagnostic tool, even though their relevance in the management of HCV infection w...
Source: Clinical Microbiology and Infection - December 11, 2014 Category: Microbiology Authors: E. Riva, C. Scagnolari, O. Turriziani, G. Antonelli Tags: Review Source Type: research

Abstracts of Online‐Only Letters
(Source: Clinical Microbiology and Infection)
Source: Clinical Microbiology and Infection - December 2, 2014 Category: Microbiology Tags: Abstracts of Online‐Only Letters Source Type: research

Abstract of Online‐Only Research Note
(Source: Clinical Microbiology and Infection)
Source: Clinical Microbiology and Infection - December 2, 2014 Category: Microbiology Tags: Abstract of Online‐Only Research Note Source Type: research

Abstract of Online‐Only Original Article
(Source: Clinical Microbiology and Infection)
Source: Clinical Microbiology and Infection - December 2, 2014 Category: Microbiology Tags: Abstract of Online‐Only Original Article Source Type: research

Abstracts of Online‐Only Original Articles
(Source: Clinical Microbiology and Infection)
Source: Clinical Microbiology and Infection - December 2, 2014 Category: Microbiology Tags: Abstracts of Online‐Only Original Articles Source Type: research