Sequence analysis of a normalized cDNA library of Mytilus edulis hemocytes exposed to Vibrio splendidus LGP32 strain
Publication date: 2013 Source:Results in Immunology, Volume 3 Author(s): Marion Tanguy , Patty McKenna , Sophie Gauthier-Clerc , Jocelyne Pellerin , Jean-Michel Danger , Ahmed Siah In the past decades, reports on bivalves' pathogens and associated mortalities have steadily increased. To face pathogenic micro-organisms, bivalves rely on innate defenses established in hemocytes which are essentially based on phagocytosis and cytotoxic reactions. As a step towards a better understanding of the molecular mechanisms involved in the mussel Mytilus edulis innate immune system, we constructed and sequenced a normalized c...
Source: Results in Immunology - November 6, 2014 Category: Allergy & Immunology Source Type: research
Proof of concept: A bioinformatic and serological screening method for identifying new peptide antigens for Chlamydia trachomatis related sequelae in women
This study aimed to identify new peptide antigens from Chlamydia (C.) trachomatis in a proof of concept approach which could be used to develop an epitope-based serological diagnostic for C. trachomatis related infertility in women. A bioinformatics analysis was conducted examining several immunodominant proteins from C. trachomatis to identify predicted immunoglobulin epitopes unique to C. trachomatis. A peptide array of these epitopes was screened against participant sera. The participants (all female) were categorized into the following cohorts based on their infection and gynecological history; acute (single treated in...
Source: Results in Immunology - November 6, 2014 Category: Allergy & Immunology Source Type: research
Development and characterization of two porcine monocyte-derived macrophage cell lines
Publication date: 2013 Source:Results in Immunology, Volume 3 Author(s): Carol G. Chitko-McKown , Stephen K. Chapes , Laura C. Miller , Penny K. Riggs , M. Teresa Ortega , Benedict T. Green , Richard D. McKown Cell lines CΔ2+ and CΔ2− were developed from monocytes obtained from a 10-month-old, crossbred, female pig. These cells morphologically resembled macrophages, stained positively for α-naphthyl esterase and negatively for peroxidase. The cell lines were bactericidal and highly phagocytic. Both cell lines expressed the porcine cell-surface molecules MHCI, CD11b, CD14, CD16, CD172, and small amounts of C...
Source: Results in Immunology - November 6, 2014 Category: Allergy & Immunology Source Type: research
Tollip-induced down-regulation of MARCH1
Publication date: 2013 Source:Results in Immunology, Volume 3 Author(s): Marie-Claude Bourgeois-Daigneault , Abdul Mohammad Pezeshki , Tristan Galbas , Mathieu Houde , Martin Baril , Klaus Früh , Abdelaziz Amrani , Satoshi Ishido , Daniel Lamarre , Jacques Thibodeau In addition to their classical antigen presenting functions, MHC class II molecules potentiate the TLR-triggered production of pro-inflammatory cytokines. Here, we have addressed the effect of Tollip and MARCH1 on the regulation of MHC II trafficking and TLR signaling. Our results show that MARCH1-deficient mice splenocytes are impaired in their ...
Source: Results in Immunology - November 6, 2014 Category: Allergy & Immunology Source Type: research
Increased production of autoantibodies and specific antibodies in response to influenza virus vaccination in physically active older individuals
Publication date: 2013 Source:Results in Immunology, Volume 3 Author(s): André L.L. Bachi , Vinicius M. Suguri , Luiz Roberto Ramos , Mario Mariano , Mauro Vaisberg , Jose D. Lopes Immunosenescence is associated to aging and among many changes in immune response is reported a reduced response to vaccination and an increase in the number of cases of autoimmunity, caused by autoantibodies known as natural antibodies whose function, according to reports, would be protection against infection and inflammation. Although immunosenescence is an irreversible process, regular moderate exercise can attenuate some aspects ...
Source: Results in Immunology - November 6, 2014 Category: Allergy & Immunology Source Type: research
Uptake of biodegradable poly(γ-glutamic acid) nanoparticles and antigen presentation by dendritic cells in vivo
In this study, γ-PGA NPs were examined for their uptake by DCs and subsequent migration from the skin to the regional lymph nodes (LNs) in mice. After subcutaneous injection of fluorescein 5-isothiocyanate (FITC)-labeled NPs or FITC-ovalbumin (OVA)-carrying NPs (FITC-OVA-NPs), DCs migrated from the skin to the LNs and maturated, resulting in the upregulation of the costimulatory molecules CD80 and CD86 and the chemokine receptor CCR7. However, the migrated DCs were not detected in the spleen. FITC-OVA-NPs were found to be taken up by skin-derived CD103+ DCs, and the processed antigen peptides were cross-presented by the m...
Source: Results in Immunology - November 6, 2014 Category: Allergy & Immunology Source Type: research
Advisory Board
Publication date: 2013 Source:Results in Immunology, Volume 3 (Source: Results in Immunology)
Source: Results in Immunology - November 6, 2014 Category: Allergy & Immunology Source Type: research
Predictive value of antinuclear antibodies in autoimmune diseases classified by clinical criteria: Analytical study in a specialized health institute, 1 year follow-up
Conclusion: Application of the test after careful evaluation for clinical criteria remarkably improves the positive likelihood ratio for the diagnosis. (Source: Results in Immunology)
Source: Results in Immunology - November 6, 2014 Category: Allergy & Immunology Source Type: research
Effective anthelmintic therapy of residents living in endemic area of high prevalence for hookworm and Schistossoma mansoni infections enhances the levels of allergy risk factor anti-Der p1 IgE
In conclusion, effective anthelmintic therapy of subjects from endemic areas with high prevalence of hookworm and S. mansoni infections enhances anti-Der p1 IgE levels. (Source: Results in Immunology)
Source: Results in Immunology - November 6, 2014 Category: Allergy & Immunology Source Type: research
Establishment of c-myc-immortalized Kupffer cell line from a C57BL/6 mouse strain
In this study, we applied this method to the C57BL/6 mouse liver and established an immortalized Kupffer cell line from this mouse strain. The hepatocytes from the C57BL/6 adult mouse liver were isolated by a two-step collagenase perfusion method and cultured in T25 culture flasks. Similar to our previous studies, the mouse hepatocytes progressively changed their morphology into a fibroblastic appearance after a few days of culture. After 7–10 days of culture, Kupffer-like cells, which were contaminants in the hepatocyte fraction at the start of the culture, actively proliferated on the mixed fibroblastic cell sheet. At...
Source: Results in Immunology - November 6, 2014 Category: Allergy & Immunology Source Type: research
Extracellular ATP does not induce P2X7 receptor-dependent responses in cultured renal- and liver-derived swine macrophages
In this study, we investigated the expression and functions of P2X7R in swine macrophages, which were isolated from mixed primary cultures of swine kidney or liver tissue. The P2X7R mRNA and protein expression observed in the swine macrophages was comparable to that seen in a c-myc-immortalized mouse kidney-derived clonal macrophage cell line (KM-1). However, extracellular ATP did not induce P2X7R-dependent sustained Ca2+ influx, membrane pore formation, or the secretion of the bioactive cytokine interleukin-1β in the swine macrophages, whereas these responses were clearly observed in the mouse KM-1 cells after stimulatio...
Source: Results in Immunology - November 6, 2014 Category: Allergy & Immunology Source Type: research
Fungal colonization with Pneumocystis correlates to increasing chloride channel accessory 1 (hCLCA1) suggesting a pathway for up-regulation of airway mucus responses, in infant lungs
Publication date: 2014 Source:Results in Immunology, Volume 4 Author(s): Francisco J. Pérez , Carolina A. Ponce , Diego A. Rojas , Pablo A. Iturra , Rebeca I. Bustamante , Myriam Gallo , Karime Hananias , Sergio L. Vargas Fungal colonization with Pneumocystis is associated with increased airway mucus in infants during their primary Pneumocystis infection, and to severity of COPD in adults. The pathogenic mechanisms are under investigation. Interestingly, increased levels of hCLCA1 – a member of the calcium-sensitive chloride conductance family of proteins that drives mucus hypersecretion – have been associ...
Source: Results in Immunology - November 6, 2014 Category: Allergy & Immunology Source Type: research
Normal serum levels of immune complexes in postpolio patients
Conclusions There was no increase in circulating immune complex or in tumor necrosis factor-inducing effects of circulating immune complex between postpolio patients and healthy controls, indicating that the postpolio syndrome is not due to an autoimmune reaction. (Source: Results in Immunology)
Source: Results in Immunology - November 6, 2014 Category: Allergy & Immunology Source Type: research
DNA-encapsulated magnesium phosphate nanoparticles elicit both humoral and cellular immune responses in mice
Publication date: 2014 Source:Results in Immunology, Volume 4 Author(s): Gajadhar Bhakta , Victor Nurcombe , Amarnath Maitra , Anju Shrivastava The efficacy of pEGFP (plasmid expressing enhanced green fluorescent protein)-encapsulated PEGylated (meaning polyethylene glycol coated) magnesium phosphate nanoparticles (referred to as MgPi-pEGFP nanoparticles) for the induction of immune responses was investigated in a mouse model. MgPi-pEGFP nanoparticles induced enhanced serum antibody and antigen-specific T-lymphocyte responses, as well as increased IFN-γ and IL-12 levels compared to naked pEGFP when administered vi...
Source: Results in Immunology - November 6, 2014 Category: Allergy & Immunology Source Type: research
Molecular pathway alterations in CD4 T-cells of nonobese diabetic (NOD) mice in the preinsulitis phase of autoimmune diabetes
Publication date: 2014 Source:Results in Immunology, Volume 4 Author(s): Dorothy N. Kakoola , Anita Curcio-Brint , Nataliya I. Lenchik , Ivan C. Gerling Type 1 diabetes (T1D) is a multigenic disease caused by T-cell mediated destruction of the insulin producing pancreatic islet β-cells. The earliest sign of islet autoimmunity in NOD mice, islet leukocytic infiltration or insulitis, is obvious at around 5 weeks of age. The molecular alterations that occur in T cells prior to insulitis and that may contribute to T1D development are poorly understood. Since CD4 T-cells are essential to T1D development, we tested the...
Source: Results in Immunology - November 6, 2014 Category: Allergy & Immunology Source Type: research
