Diversity of CD2 subfamily receptors in cyprinid fishes
Publication date: 2012 Source:Results in Immunology, Volume 2 Author(s): Shiro Sameshima , Miki Nakao , Tomonori Somamoto CD2 family receptor (CD2f) is evolutionarily conserved and is widely expressed by various types of leukocytes. To elucidate the phylogenetic diversity of the CD2f, we characterized CD2f in teleosts using ginbuna crucian carp and zebrafish. The identified CD2f isoforms of the ginbuna carp (caauCD2f) exhibited high sequence similarity to the mammalian CD2 subsets CD48, CD244, and CD319, but it was difficult to classify them into their respective mammalian CD2f based on sequence similarity, the pres...
Source: Results in Immunology - November 6, 2014 Category: Allergy & Immunology Source Type: research
Effective virus inactivation and removal by steps of Biotest Pharmaceuticals IGIV production process
Publication date: 2012 Source:Results in Immunology, Volume 2 Author(s): Herbert O. Dichtelmüller , Eckhard Flechsig , Frank Sananes , Michael Kretschmar , Christopher J. Dougherty The virus validation of three steps of Biotest Pharmaceuticals IGIV production process is described here. The steps validated are precipitation and removal of fraction III of the cold ethanol fractionation process, solvent/detergent treatment and 35nm virus filtration. Virus validation was performed considering combined worst case conditions. By these validated steps sufficient virus inactivation/removal is achieved, resulting in a vir...
Source: Results in Immunology - November 6, 2014 Category: Allergy & Immunology Source Type: research
IgM phosphorylcholine antibodies inhibit cell death and constitute a strong protection marker for atherosclerosis development, particularly in combination with other auto-antibodies against modified LDL
Conclusion Group I anti-PC antibodies, particularly of the IgM class, are independent protection markers for atherosclerosis progression. One potential mechanism of action is inhibition of LPC-induced cell cytotoxicity. Highlights ► Anti-PC IgM, IgA and IgG1 (but not IgG2) protects against IMT-progression. ► Combining anti-PC with other antibodies against oxLDL strengthens this association. ► The protective anti-PC isotypes belong to the Group I idiotype. ► Serum levels of anti-PC IgM are stable over time. (Source: Results in Immunology)
Source: Results in Immunology - November 6, 2014 Category: Allergy & Immunology Source Type: research
Hepatocyte growth factor in patients with coronary artery disease and its relation to periodontal condition
Publication date: 2012 Source:Results in Immunology, Volume 2 Author(s): J. Lönn , C. Starkhammar Johansson , H. Kälvegren , L. Brudin , C. Skoglund , P. Garvin , E. Särndahl , N. Ravald , A. Richter , T. Bengtsson , F. Nayeri Hepatocyte growth factor (HGF) is an angiogenic, cardioprotective factor important for tissue and vascular repair. High levels of HGF are associated with chronic inflammatory diseases, such as coronary artery disease (CAD) and periodontitis, and are suggested as a marker of the ongoing atherosclerotic event in patients with CAD. Periodontal disease is more prevalent among patients w...
Source: Results in Immunology - November 6, 2014 Category: Allergy & Immunology Source Type: research
Effect of treatment on systemic cytokines in head and neck squamous cell carcinoma patients
Publication date: 2012 Source:Results in Immunology, Volume 2 Author(s): Victoria L. Green , Anna Michno , John Greenman , Nicholas D. Stafford The aim of this study was to determine the effect of HNSCC tumour treatment on systemic Th1 and Th2 cytokine levels and investigate correlations with clinicopathological parameters. IL2, IL4, IL5, IL6, IL8, IL10, IL13, GMCSF, IFNγ and TNFα were measured in the serum of 101 newly-presenting HNSCC patients (9 oral cavity, 27 oropharynx, 57 laryngopharynx, 1 sinonasal, 1 parotid and 6 unknown), prior to and following treatment, using a Quantibody® array based multiplex sand...
Source: Results in Immunology - November 6, 2014 Category: Allergy & Immunology Source Type: research
Perturbations of the CD8+ T-cell repertoire in CVID patients with complications
Publication date: 2013 Source:Results in Immunology, Volume 3 Author(s): Jean-François Viallard , Catherine Ruiz , Marina Guillet , Jean-Luc Pellegrin , Jean-François Moreau A higher chronic expansion of effector cytotoxic CD8+DR+ T-lymphocytes has been reported in common variable immunodeficiency (CVID) patients with complications such as splenomegaly, autoimmune disease and/or granulomatous disease. In order to document the features associated with this T cell activation involving the CD8+ T-compartment, we examined the diversity of the alpha/beta TCR repertoire of the patient's CD8+ T-lymphocytes using the qu...
Source: Results in Immunology - November 6, 2014 Category: Allergy & Immunology Source Type: research
Complement regulator C4BP binds to Staphylococcus aureus surface proteins SdrE and Bbp inhibiting bacterial opsonization and killing
Publication date: 2013 Source:Results in Immunology, Volume 3 Author(s): Pamela S. Hair , Caitlin K. Foley , Neel K. Krishna , Julius O. Nyalwidhe , Joan A. Geoghegan , Timothy J. Foster , Kenji M. Cunnion Staphylococcus aureus is a premier human pathogen and the most common cause of osteoarticular, wound, and implanted device infections. We recently demonstrated S. aureus efficiently binds the classical complement regulator C4b-binding protein (C4BP) inhibiting antibody-initiated complement-mediated opsonization. Here we identify S. aureus surface protein SdrE as a C4BP-binding protein. Recombinant SdrE and re...
Source: Results in Immunology - November 6, 2014 Category: Allergy & Immunology Source Type: research
CD4 and CD8 T cells participate in the immune memory response against Vaccinia virus after a previous natural infection
Publication date: 2013 Source:Results in Immunology, Volume 3 Author(s): Daniela Carla Medeiros-Silva , Eduardo Augusto dos Santos Moreira-Silva , Juliana de Assis Silva Gomes , Flávio Guimarães da Fonseca , Rodrigo Correa-Oliveira The present study evaluates the immune response of memory CD4+ and CD8+ T cells from patients following a natural Vaccinia virus (VACV) infection. A total of 42 individuals were involved in the study being: 22 previously infected individuals (vaccinated or not against smallpox) and 20 non-infected individuals (vaccinated or not). A short-term in vitro stimulation with UV-inactivated V...
Source: Results in Immunology - November 6, 2014 Category: Allergy & Immunology Source Type: research
Transcriptome analysis of neoplastic hemocytes in soft-shell clams Mya arenaria: Focus on cell cycle molecular mechanism
This study aims at identifying the transcripts related to DN in soft shell clams’ hemocytes using next generation of sequencing (Illumina HiSeq2000). This study mainly focuses on transcripts and molecular mechanisms involved in cell cycle. Using Illumina next generation of sequencing, more than 95,399,159 reads count with an average length of 45 bp was generated from three groups of hemocytes: (1) a healthy group with less than 10% of tetraploid cells; (2) an intermediate group with tetraploid hemocytes ranging between 10% and 50% and (3) a diseased group with more than 50% of tetraploid cells. After the reads were ...
Source: Results in Immunology - November 6, 2014 Category: Allergy & Immunology Source Type: research
Burn-injury affects gut-associated lymphoid tissues derived CD4+ T cells
Publication date: 2013 Source:Results in Immunology, Volume 3 Author(s): Nadeem Fazal , Alla Shelip , Alhusain J. Alzahrani After scald burn-injury, the intestinal immune system responds to maintain immune balance. In this regard CD4+T cells in Gut-Associated Lymphoid Tissues (GALT), like mesenteric lymph nodes (MLN) and Peyer's patches (PP) respond to avoid immune suppression following major injury such as burn. Therefore, we hypothesized that the gut CD4+T cells become dysfunctional and turn the immune homeostasis towards depression of CD4+ T cell-mediated adaptive immune responses. In the current study we show do...
Source: Results in Immunology - November 6, 2014 Category: Allergy & Immunology Source Type: research
Detectability and reproducibility of plasma levels of chemokines and soluble receptors
Conclusion: Multiplex assays for plasma levels of selected chemokines and soluble receptors showed good to excellent assay detectability and repeatability. Most analytes also had good 3-year within-subject reproducibility, indicating that a single measurement of these analytes may be used to assess biomarker-disease associations. (Source: Results in Immunology)
Source: Results in Immunology - November 6, 2014 Category: Allergy & Immunology Source Type: research
Shell colour polymorphism, injuries and immune defense in three helicid snail species, Cepaea hortensis, Theba pisana and Cornu aspersum maximum
Publication date: 2013 Source:Results in Immunology, Volume 3 Author(s): Alexandra E. Scheil , Stefanie Hilsmann , Rita Triebskorn , Heinz-R. Köhler Shell colour polymorphism is a widespread feature of various land snail species. In our study we aimed at elucidating the question whether there is a correlation between shell colouration and immune defense in three land snail species by comparing phenoloxidase (PO) activity levels of different morphs after immunostimulation via Zymosan A-injection. Since phenoloxidase is involved both in immune defense as well as in melanin production, the PO activity level is partic...
Source: Results in Immunology - November 6, 2014 Category: Allergy & Immunology Source Type: research
OX62+OX6+OX35+ rat dendritic cells are unable to prime CD4+ T cells for an effective immune response following acute burn injury
Publication date: 2013 Source:Results in Immunology, Volume 3 Author(s): Nadeem Fazal Co-stimulatory molecules expressed on Dendritic Cells (DCs) function to coordinate an efficient immune response by T cells in the peripheral lymph nodes. We hypothesized that CD4+ T cell-mediated immune suppression following burn injury may be related to dysfunctional DCs residing in gut associated lymphoid tissues (GALT), such as Mesenteric Lymph Nodes (MLN). Therefore, we studied co-stimulatory molecules expressed on burn rat MLN DCs as an index of functional DCs that would mount an effective normal CD4+ T cell immune response. In ...
Source: Results in Immunology - November 6, 2014 Category: Allergy & Immunology Source Type: research
Molecular characterization and validation of commercially available methods for haptoglobin measurement in bottlenose dolphin
Publication date: 2013 Source:Results in Immunology, Volume 3 Author(s): Takao Segawa , Hazumu Amatsuji , Kento Suzuki , Miwa Suzuki , Makio Yanagisawa , Takuya Itou , Takeo Sakai , Teruyuki Nakanishi Haptoglobin (Hp) is a positive acute-phase protein and a valuable marker of inflammation in both human and veterinary medicine. The aim of this study was to validate the molecular characterization of Hp in dolphins and to validate commercially available Hp measurement methods such as Hp-ELISA (originally designed for pigs) and Hp–hemoglobin (Hb) binding assay. The dolphin Hp (dHp) amino acid sequence appeared mo...
Source: Results in Immunology - November 6, 2014 Category: Allergy & Immunology Source Type: research
Development of an ELISA detecting Tumor Protein 53-Induced Nuclear Protein 1 in serum of prostate cancer patients
We report here successful development of a new Enzyme-Linked Immunosorbent Assay (ELISA) detecting TP53INP1, taking advantage of molecular tools (monoclonal antibodies (mAbs) and recombinant proteins) generated in the laboratory during the course of basic functional investigations devoted to TP53INP1. The ELISA principle is based on a sandwich immunoenzymatic system, TP53INP1 protein being trapped by a first specific mAb coated on microplate then recognized by a second specific mAb. This new assay allows specific detection of TP53INP1 in serum of several PC patients. This breakthrough paves the way towards investigation of...
Source: Results in Immunology - November 6, 2014 Category: Allergy & Immunology Source Type: research
