Complete mapping of disulfide linkages for etanercept products by multi-enzyme digestion coupled with LC-MS/MS using multi-fragmentations including CID and ETD
Publication date: Available online 2 January 2019Source: Journal of Food and Drug AnalysisAuthor(s): Li-Juan Huang, Chia-Wang Chiang, Shun-Li Chen, Shih-Yao Wei, Shu-Hui ChenAbstractThe disulfide linkages of two etanercept products, Enbrel® (innovator drug) and TuNEX®, were characterized and compared using a multi-fragmentation approach consisting of electron transfer dissociation (ETD) and collision induced dissociation (CID) in combination with multi-enzyme digestion protocols (from Lys-C, trypsin, Glu-C, and PNGase F). Multi-fragmentation approach allowed multi-disulfide linkages contained in a peptide to be un-ambigu...
Source: Journal of Food and Drug Analysis - January 3, 2019 Category: Food Science Source Type: research
Quantitation of serum 25(OH)D2 and 25(OH)D3 concentrations by liquid chromatography tandem mass spectrometry in patients with diabetes mellitus
Publication date: Available online 31 December 2018Source: Journal of Food and Drug AnalysisAuthor(s): Yi-Ching Lin, Hei-Hwa Lee, Shing-Cheng Tseng, Kun-Der Lin, Li-Ping Tseng, Jong-Feng Lee, Yung-Hung Lee, Bai-Hsiun ChenAbstractVitamin D has been considered to regulate calcium and phosphorus homeostasis and to preserve skeletal integrity. Serum 25-hydroxyvitamin D (25(OH)D) is the best indicator of vitamin D levels. The association of serum 25(OH)D deficiency with increased risk of type 1 diabetes (T1DM) and type 2 diabetes (T2DM) is controversial. We investigated serum 25(OH)D2 and 25(OH)D3 levels in diabetes patients by...
Source: Journal of Food and Drug Analysis - December 31, 2018 Category: Food Science Source Type: research
Simultaneous quantification of six indicator compounds in Wen-Qing-Yin by high-performance liquid chromatography-diode array detection
Publication date: Available online 28 December 2018Source: Journal of Food and Drug AnalysisAuthor(s): Chi-Chen Yeh, Shih-Shan Huang, Po-Yu Liu, Bo-Cheng Wang, Chia-Fen Tsai, Der-Yuan Wang, Hwei-Fang ChengAbstractA simple gradient high-performance liquid chromatography with diode array detection (HPLC-DAD) method was used to simultaneously to analyze characteristics of six indicator compounds in the traditional Chinese medicine (TCM) formulation Wen-Qing-Yin (WQY). Separate optimization was performed using a Cosmosil C18 column gradient method with 0.1% formic acid in both mobile phases of aqueous and acetonitrile (ACN), a...
Source: Journal of Food and Drug Analysis - December 29, 2018 Category: Food Science Source Type: research
The current application of nanotechnology in food and agriculture
Publication date: Available online 24 December 2018Source: Journal of Food and Drug AnalysisAuthor(s): Xiaojia He, Hua Deng, Huey-min HwangAbstractThe rapid development of nanotechnology has been facilitating the transformations of traditional food and agriculture sectors, particularly the invention of smart and active packaging, nanosensors, nanopesticides and nanofertilizers. Numerous novel nanomaterials have been developed for improving food quality and safety, crop growth, and monitoring environmental conditions. In this review the most recent trends in nanotechnology are discussed and the most challenging tasks and pr...
Source: Journal of Food and Drug Analysis - December 25, 2018 Category: Food Science Source Type: research
Exposure marker discovery of di-2(propylheptyl) phthalate using ultra-performance liquid chromatography-mass spectrometry and a rat model
Publication date: Available online 11 December 2018Source: Journal of Food and Drug AnalysisAuthor(s): Chia-Lung Shih, Jen-Yi Hsu, Chien-Ping Tien, Yi-Ning Chung, Victor G. Zgoda, Pao-Chi LiaoAbstractDi-(2-propylheptyl) phthalate (DPHP) is a plasticizer and has been suggested to be a subchronic toxicant in rats. DPHP has been approved to be used in food containers and handling by the U.S. Food and Drug Administration. The use of DPHP is still increasing, and the risk of human exposure to DPHP via food may be high. Exposure markers measured in human samples are commonly used to monitor human exposure levels. Ultra-performan...
Source: Journal of Food and Drug Analysis - December 12, 2018 Category: Food Science Source Type: research
Chemical isotope labeling liquid chromatography mass spectrometry for investigating acute dietary effects of cow milk consumption on human urine metabolome
Publication date: Available online 11 December 2018Source: Journal of Food and Drug AnalysisAuthor(s): Dorothea Mung, Liang LiAbstractBiomarker discovery has been increasingly important in the field of metabolomics for the detection and understanding of diseases. Of the many biofluids available for metabolomics, urine is a preferred option as it is non-invasive to collect and contains a wide range of metabolites reflective of the health status of the testing individual. However, urine also contains many exogenous metabolites which are introduced through various sources such as diet. This complicates the data interpretation...
Source: Journal of Food and Drug Analysis - December 12, 2018 Category: Food Science Source Type: research
Using ambient mass spectrometry and LC–MS/MS for the rapid detection and identification of multiple illicit street drugs
In this study the recently developed technique of thermal desorption electrospray ionization/mass spectrometry (TD–ESI/MS) was applied to the rapid analysis of multiple controlled substances. With the reallocation of mass spectral resources [from a standard ESI source coupled with liquid chromatography (LC) to an ambient TD–ESI source], this direct-analysis technique allows the identification of a wider range of illicit drugs through a dual-working mode (pretreatment-free qualitative screening/conventional quantitative confirmation). Through 60-MRM (multiple reaction monitoring) analysis—in which the MS/MS process wa...
Source: Journal of Food and Drug Analysis - December 5, 2018 Category: Food Science Source Type: research
Measuring serum total and free indoxyl sulfate and p-cresyl sulfate in chronic kidney disease using UPLC-MS/MS
Publication date: Available online 4 December 2018Source: Journal of Food and Drug AnalysisAuthor(s): Chia-Ni Lin, I-Wen Wu, Yun-Fen Huang, Shu-Yu Peng, Ya-Ching Huang, Hsiao-Chen NingAbstractChronic kidney disease (CKD) is a complex disorder that affects multiple organs and increases the risk of cardiovascular complications. CKD affects approximately 12% of the population in Taiwan. Loss of kidney function leads to accumulation of potentially toxic compounds such as indoxyl sulfate (IS) and p-cresyl sulfate (pCS), two protein-bound uremic solutes that can stimulate the progression of CKD. The aim of this study was to asse...
Source: Journal of Food and Drug Analysis - December 5, 2018 Category: Food Science Source Type: research
The regulation of drug-metabolizing enzymes and membrane transporters by inflammation: Evidences in inflammatory diseases and age-related disorders
Publication date: Available online 4 December 2018Source: Journal of Food and Drug AnalysisAuthor(s): Kuo-Chen Wu, Chun-Jung LinAbstractDrug-metabolizing enzymes (DMEs) and membrane transporters play important roles in the absorption, distribution, metabolism, and excretion processes that determine the pharmacokinetics of drugs. Inflammation has been shown to regulate the expression and function of these drug-processing proteins. Given that inflammation is a common feature of many diseases, in this review, the general mechanisms for inflammation-mediated regulation of DMEs and transporters are described. Also, evidences re...
Source: Journal of Food and Drug Analysis - December 5, 2018 Category: Food Science Source Type: research
Antcins, triterpenoids from Antrodia cinnamomea, as new agonists for peroxisome proliferator-activated receptor α
Publication date: Available online 4 December 2018Source: Journal of Food and Drug AnalysisAuthor(s): Yu-Jen Wang, Shih-Chin Lee, Chun-Hua Hsu, Yueh-Hsiung Kuo, Chien-Chih Yang, Fu-Jung LinAbstractPeroxisome proliferator-activated receptor α (PPARα) is a nuclear hormone receptor that transcriptionally regulates lipid metabolism and inflammation; therefore, PPARα agonists are promising agents to treat dyslipidemia and metabolic disorders. PPARα full agonists, such as fibrates, are effective anti-hypertriglyceride agents, but their use is limited by adverse side effects. Hence, the aim of this study was to identify small...
Source: Journal of Food and Drug Analysis - December 4, 2018 Category: Food Science Source Type: research
Shenmai-Yin decreased the clearance of nifedipine in rats: The involvement of time-dependent inhibition of nifedipine oxidation
Publication date: Available online 8 November 2018Source: Journal of Food and Drug AnalysisAuthor(s): Hong-Jaan Wang, Chung-Kuang Lu, Wei-Ching Chen, An-Chi Chen, Yune-Fang UengAbstractThe traditional Chinese herbal formula Shenmai-Yin (SY) and nifedipine have both been used to treat patients with cardiovascular disorders. Nifedipine is primarily oxidized by cytochrome P450 (CYP) 3A. The oxidation and pharmacokinetics of nifedipine were studied in rats in vitro and in vivo to illustrate the interaction of SY with nifedipine. Schisandrol A, schisandrin A and schisandrin B were identified as the main lignans in SY. In the ...
Source: Journal of Food and Drug Analysis - November 9, 2018 Category: Food Science Source Type: research
Comparative investigation for raw and processed Aconiti Lateralis Radix using chemical UPLC-MS profiling and multivariate classification techniques
Publication date: Available online 8 November 2018Source: Journal of Food and Drug AnalysisAuthor(s): Lili Sun, Guangjiao You, Xuexiao Cao, Meng Wang, Xiaoliang RenAbstractA strategy combining chemical UPLC-MS profiling and multivariate classification techniques has been used for the comparison of raw and processed Aconiti Lateralis Radix. UPLC-MS was used to identify 18 characteristic compounds, which were selected for discrimination of the raw and two processed products (Heishunpian and Baifupian). Chemometric analyses, including the combination of a heat map and hierarchical cluster analysis (HCA) and principal componen...
Source: Journal of Food and Drug Analysis - November 9, 2018 Category: Food Science Source Type: research
PON-1 carbamylation is enhanced in HDL of uremia patients
Publication date: Available online 28 October 2018Source: Journal of Food and Drug AnalysisAuthor(s): Chiz-Tzung Chang, Yun-Ping Lim, Chi-Wen Lee, Hsin-Yi Liao, Feng-Yu Chen, Chia-Ming Chang, Feng-Yao Tang, Chao-Yuh Yang, Chao-Jung ChenAbstractHigh-density lipoprotein (HDL) carbamylation has been known in uremia patients. Paraoxonase-1 (PON-1) is an important HDL protein responsible for HDL anti-oxidant, arylesterase and lactonase activities. PON-1 carbamylation in uremic HDL has never been explored. We isolated HDL from uremia patients and control healthy subjects for study. Sandwich ELISA was used to estimate carbamylate...
Source: Journal of Food and Drug Analysis - October 29, 2018 Category: Food Science Source Type: research
Method development of immunoglobulin G purification from micro-volumes of human serum for untargeted and targeted proteomics-based antibody repertoire studies
Publication date: Available online 27 October 2018Source: Journal of Food and Drug AnalysisAuthor(s): Yu-Ting Chang, Ming-Chu Chang, Yun-Jun Tsai, Christine Ferng, Hsi-Chang Shih, Ya-Po Kuo, Chung-Hsuan Chen, I-Lin TsaiAbstractImmunoglobulins (Igs) are major serum proteins which play important roles in immunity. Both untargeted and targeted proteomic workflows can be applied to investigate antigen-binding sites and the glycosylation profiles of Igs. For a more-comprehensive picture of IgG from human serum, we developed an IgG purification process and coupled the standardized method to untargeted and targeted proteomic work...
Source: Journal of Food and Drug Analysis - October 27, 2018 Category: Food Science Source Type: research
Analysis of heterocyclic amines in meat products by liquid chromatography – Tandem mass spectrometry
In this study, two sample preparation strategies, liquid–liquid extraction (LLE) with solid-phase extraction (SPE) and a rapid, easy, cheap, effective, rugged, and safe extraction (QuEChERS) method, were investigated for the determination of 11 types of HCAs in meat products by LC-MS/MS. The HCAs in the samples were first extracted with acetonitrile by LLE, and followed by SPE. In the case of QuEChERS extraction, acetonitrile is used as the LLME solvent, and PSA, C18EC and MgSO4 were used as the dSPE sorbent. Both methods showed good performance with respect to precision (RSD < 15.15%), accuracy (79.80–117.64%), ...
Source: Journal of Food and Drug Analysis - October 27, 2018 Category: Food Science Source Type: research
