Production of functional peptides with inhibition ability against angiotensin I-Converting enzyme using P. pastoris expression system
Publication date: July 2018Source: Journal of Food and Drug Analysis, Volume 26, Issue 3Author(s): Hsueh-Ming Tai, Ching-Chin Li, Chun-Yu Hung, Li-Jung YinAbstractTo obtain the angiotension-I converting enzyme inhibitor (ACEI), a fusion ACEI polypeptide encoded with 8 DNA sequences of GPL, GPM, IKW, IVY, IRPVQ, IWHHT, IYPRY and IAPG, which were selected and designed and cloned into pGAPZαC and then transformed into Pichia pastoris SMD1168H. After 3 days induction, the fraction with highest ACEI activity was expressed and purified using a Ni Sepharose™ 6 Fast Flow. The IC50 of recombinant ACEI polypeptide was 88.2 μM. ...
Source: Journal of Food and Drug Analysis - July 5, 2018 Category: Food Science Source Type: research
A feasible and practical 1H NMR analytical method for the quality control and quantification of bioactive principles in Lycii Fructus
Publication date: July 2018Source: Journal of Food and Drug Analysis, Volume 26, Issue 3Author(s): Li-Ying Hsieh, Hsiu-Hui Chan, Ping-Chung Kuo, Hsin-Yi Hung, Yue-Chiun Li, Chao-Lin Kuo, Yong Peng, Zhong-Zhen Zhao, Daih-Huang Kuo, I-Wen Sun, Tian-Shung WuAbstractLycii Fructus, a solanaceous drug, is widely used as functional foods and in Traditional Chinese Medicine. Samples collected from different regions of China have been found to be not identical in chemical compositions which might affect the biological activities. Although many chromatographic and spectrometric methods have been reported to determine the concentrati...
Source: Journal of Food and Drug Analysis - July 5, 2018 Category: Food Science Source Type: research
Quantitative and qualitative analyses of cytotoxic triterpenoids in the rhizomes of Anemone raddeana using HPLC and HPLC-ESI-Q/TOF-MS
Publication date: July 2018Source: Journal of Food and Drug Analysis, Volume 26, Issue 3Author(s): Ying Zhao, Xin Zhang, Chongning Lv, Yang Yu, Yu Zhang, Jincai LuAbstractAnemone raddeana Regel, a Traditional Chinese Medicine, has been demonstrated to possess cytotoxicity and anti-inflammatory activities. The purpose of this study is to establish analytical methods to identify and quantify the major active constituents in Anemone raddeana. A high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (HPLC-ESI-Q/TOF-MS) was used to identify the components i...
Source: Journal of Food and Drug Analysis - July 5, 2018 Category: Food Science Source Type: research
Comprehensive quality evaluation and comparison of Angelica sinensis radix and Angelica acutiloba radix by integrated metabolomics and glycomics
In this study, untargeted/targeted metabolomics and glycomics were integrated by multiple chromatography-based analytical techniques for qualitative and quantitative characterization of secondary metabolites and carbohydrates in Danggui so as to comprehensively evaluate and compare the quality of ASR and AAR. The results revealed that not only secondary metabolites but also carbohydrates in ASR and AAR were different in type and amount, which should collectively contribute to their quality difference. By providing more comprehensive chemical information, the research results highlighted the need to assess characteristics o...
Source: Journal of Food and Drug Analysis - July 5, 2018 Category: Food Science Source Type: research
Simultaneous analysis of 23 illegal adulterated aphrodisiac chemical ingredients in health foods and Chinese traditional patent medicines by ultrahigh performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry
Publication date: July 2018Source: Journal of Food and Drug Analysis, Volume 26, Issue 3Author(s): Xiao-Bing Wang, Jing Zheng, Jun-Jie Li, Hai-Ying Yu, Qi-Yan Li, Li-Hua Xu, Ming-Jie Liu, Rui-Qing Xian, Yang-En Sun, Bing-Jie LiuAbstractThis paper presents an application of ultra high-performance liquid-chromatography-quadrupole-TOF high resolution mass spectrometry (UHPLC-Q-TOF HRMS) for simultaneous analysis of 23 illegal adulterated aphrodisiac type chemical ingredients in health foods and Chinese Traditional Patent Medicines (CTPMs). The mass spectrometer was operated in Information Dependent Acquisition (IDA) mode, whi...
Source: Journal of Food and Drug Analysis - July 5, 2018 Category: Food Science Source Type: research
