Establishment and characterization of a new cell line from koi brain (Cyprinus carpio L.)
Abstract
A novel permanently growing brain cell line from koi (Cyprinus carpio L.) (KB cell line) was established, and its suitability for detection of koi herpesvirus (KHV) was demonstrated in this study. The KB cell line was optimally maintained at 27°C in Leibovitz's L‐15 medium supplemented with 10% foetal bovine serum (FBS). It was subcultured more than 100 times, and chromosome analysis revealed that 51.54% of KB cells at passage 80 maintained the abnormal diploid chromosome number 2n = 96 while the modal chromosome number was 2n = 100. The cell line was cryopreserved in liquid nitrogen at −196°C and was re...
Source: Journal of Fish Diseases - October 24, 2017 Category: Zoology Authors: Y Wang, W Zeng, Q Wang, Y Li, S M Bergmann, S Zheng, Y Ren, C Liu, O Chang, P Lee Tags: ORIGINAL ARTICLE Source Type: research
Cardiomyopathy syndrome in Atlantic salmon Salmo salar L.: A review of the current state of knowledge
Abstract
Cardiomyopathy syndrome (CMS) is a severe cardiac disease affecting Atlantic salmon Salmo salar L. The disease was first recognized in farmed Atlantic salmon in Norway in 1985 and subsequently in farmed salmon in the Faroe Islands, Scotland and Ireland. CMS has also been described in wild Atlantic salmon in Norway. The demonstration of CMS as a transmissible disease in 2009, and the subsequent detection and initial characterization of piscine myocarditis virus (PMCV) in 2010 and 2011 were significant discoveries that gave new impetus to the CMS research. In Norway, CMS usually causes mortality in large salmon in o...
Source: Journal of Fish Diseases - October 24, 2017 Category: Zoology Authors: Å H Garseth, C Fritsvold, J C Svendsen, B Bang Jensen, A B Mikalsen Tags: REVIEW Source Type: research
Effects of clioquinol on the scuticociliatosis ‐causing protozoan Miamiensis avidus in olive flounder Paralichthys olivaceus
This study aimed to investigate a systemically effective antiscuticociliatosis agent for olive flounder for better absorption into the infected internal organs. The in vitro and in vivo antiscuticociliatosis effects of clioquinol (CQ) were examined after screening 30 biocidal agents against the highly pathogenic scuticociliate Miamiensis avidus. CQ was the most potent in vitro drug of those tested against cultured M. avidus. CQ was the least toxic in healthy olive flounder among the drugs that exhibit high potencies. In olive flounder, a single intramuscular injection of 40 mg/kg CQ significantly reduced mortality cau...
Source: Journal of Fish Diseases - October 17, 2017 Category: Zoology Authors: J ‐H Lee, J‐J Park, J‐H Choi, S‐Y Kang, Y‐J Kang, K H Park Tags: ORIGINAL ARTICLE Source Type: research
Serum adenosine deaminase and xanthine oxidase activities in silver catfish naturally infected with Ichthyophthirius multifiliis: The influence of these enzymes on inflammatory and oxidative status
Abstract
Adenosine deaminase (ADA) activity, through adenosine (Ado) levels, as well as xanthine oxidase (XO) activity through uric acid levels exerts an essential role on immune and inflammatory responses during infectious diseases. Thus, the aim of this study was to evaluate the involvement of seric ADA and XO activities in the inflammatory and oxidative status of silver catfish naturally infected with Ichthyophthirius multifiliis. Seric ADA activity decreased, while Ado levels increased in infected animals compared to uninfected animals. Moreover, the seric XO activity increased in infected animals compared to uninfecte...
Source: Journal of Fish Diseases - October 13, 2017 Category: Zoology Authors: M D Baldissera, C F Souza, P H Doleski, S G Monteiro, A S Silva, B Baldisserotto Tags: ORIGINAL ARTICLE Source Type: research
The drivers of sea lice management policies and how best to integrate them into a risk management strategy: An ecosystem approach to sea lice management
Journal of Fish Diseases, EarlyView. (Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - October 13, 2017 Category: Zoology Source Type: research
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Journal of Fish Diseases, Ahead of Print. (Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - October 13, 2017 Category: Zoology Source Type: research
Development and validation of a reverse transcription quantitative polymerase chain reaction for tilapia lake virus detection in clinical samples and experimentally challenged fish
In this study, a SYBR green‐based reverse transcription quantitative polymerase chain reaction (RT‐qPCR) assay targeting segment three of the virus was developed to detect and quantify TiLV in clinical samples and experimentally challenged fish. All 30 field samples with clinical signs and history consistent with TiLV infection were positive for TiLV as detected by the developed RT‐qPCR method. The RT‐qPCR technique provided 100 and 10,000 times more sensitive for virus detection than those offered by the RT‐PCR and virus isolation in cell culture methods, respectively. The detection limit of the RT‐qPCR method...
Source: Journal of Fish Diseases - October 13, 2017 Category: Zoology Authors: P Tattiyapong, K Sirikanchana, W Surachetpong Tags: ORIGINAL ARTICLE Source Type: research
High prevalence of betanodavirus barfin flounder nervous necrosis virus as well as red ‐spotted grouper nervous necrosis virus genotype in shellfish
Abstract
Using two serially executed PCRs, the discriminative multiplex two‐step RT‐PCR (DMT‐2 RT‐PCR) following the detection seminested two‐step RT‐PCR (DSN‐2 RT‐PCR), we found a high frequency presence of BFNNV genotype as well as RGNNV in various domestic and imported shellfish. This was definitely different from the previous reports of outbreaks and asymptomatic infection only by the RGNNV genotype in cultured finfish in Korea. Cultivation of NNV entrapped in shellfish was performed successfully by a blind passage. Thus, in an attempt to elucidate the epidemiology of betanodavirus, experiments conducte...
Source: Journal of Fish Diseases - October 13, 2017 Category: Zoology Authors: Y C Kim, W J Kwon, M S Kim, K I Kim, J G Min, H D Jeong Tags: ORIGINAL ARTICLE Source Type: research
The drivers of sea lice management policies and how best to integrate them into a risk management strategy: An ecosystem approach to sea lice management
Abstract
The control of sea lice infestations on cultivated Atlantic salmon is a major issue in many regions of the world. The numerous drivers which shape the priorities and objectives of the control strategies vary for different regions/jurisdictions. These range from the animal welfare and economic priorities of the producers, to the mitigation of any potential impacts on wild stocks. Veterinary ethics, environmental impacts of therapeutants, and impacts for organic certification of the produce are, amongst others, additional sets of factors which should be considered. Current best practice in both EU and international ...
Source: Journal of Fish Diseases - October 13, 2017 Category: Zoology Authors: D Jackson, O Moberg, E M Stenevik Djupev åg, F Kane, H Hareide Tags: SPECIAL ISSUE Source Type: research
Transmission of a common intestinal neoplasm in zebrafish by cohabitation
This study indicates an infectious aetiology for these transmissible tumours of zebrafish and suggests a possible candidate agent of a Mycoplasma species. (Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - October 11, 2017 Category: Zoology Authors: A R Burns, V Watral, S Sichel, S Spagnoli, A V Banse, E Mittge, T J Sharpton, K Guillemin, M L Kent Tags: ORIGINAL ARTICLE Source Type: research
Transmission of a common intestinal neoplasm in zebrafish by cohabitation
Journal of Fish Diseases,Volume 41, Issue 4, Page 569-579, April 2018. (Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - October 11, 2017 Category: Zoology Source Type: research
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Journal of Fish Diseases,Volume 41, Issue 4, Page 569-579, April 2018. (Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - October 11, 2017 Category: Zoology Source Type: research
Hydrogen peroxide treatment modulates the transcription of sex ‐related genes in the sea lice Caligus rogercresseyi
Journal of Fish Diseases, EarlyView. (Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - October 6, 2017 Category: Zoology Source Type: research
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Journal of Fish Diseases, Ahead of Print. (Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - October 6, 2017 Category: Zoology Source Type: research
Hydrogen peroxide treatment modulates the transcription of sex ‐related genes in the sea lice Caligus rogercresseyi
(Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - October 1, 2017 Category: Zoology Authors: R Farlora, F Valdebenito ‐Aguayo, V Valenzuela‐Muñoz, C Gallardo‐Escárate Tags: SPECIAL ISSUE Source Type: research
