The mutagenesis of a type IV secretion system locus of Piscirickettsia salmonis leads to the attenuation of the pathogen in Atlantic salmon, Salmo salar
We report here the genetic manipulation of P. salmonis by means of the transference of plasmid DNA in mating assays. An insertion cassette was engineered for targeting the icmB gene, which encodes a putative T4SS‐ATPase and is carried by one of the chromosomal T4SS clusters found within the genome of P. salmonis PM15972A1, a virulent representative of the EM‐90‐like strain. The molecular characterization of the resulting mutant strain demonstrated that the insertion interrupted the target gene. Further in vitro testing of the icmB mutant showed a dramatic drop in infectivity as tested in CHSE‐214 cells, which is...
Source: Journal of Fish Diseases - December 18, 2017 Category: Zoology Authors: M Mancilla, J Saavedra, M Grand ón, E Tapia, E Navas, H Grothusen, P Bustos Tags: ORIGINAL ARTICLE Source Type: research
Sensitivity assessment of sea lice to chemotherapeutants: Current bioassays and best practices
Journal of Fish Diseases, EarlyView. (Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - December 18, 2017 Category: Zoology Source Type: research
The mutagenesis of a type IV secretion system locus of Piscirickettsia salmonis leads to the attenuation of the pathogen in Atlantic salmon, Salmo salar
Journal of Fish Diseases,Volume 41, Issue 4, Page 625-634, April 2018. (Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - December 18, 2017 Category: Zoology Source Type: research
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Journal of Fish Diseases, Ahead of Print. (Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - December 18, 2017 Category: Zoology Source Type: research
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Journal of Fish Diseases,Volume 41, Issue 4, Page 625-634, April 2018. (Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - December 18, 2017 Category: Zoology Source Type: research
Sensitivity assessment of sea lice to chemotherapeutants: Current bioassays and best practices
The objective of this article is to provide a baseline analysis of the methodology for traditional bioassays and to identify procedures that need to be addressed to standardize the protocol. The methodology was divided into the following steps: bioassay design; material and equipment; sea lice collection, transportation and laboratory reception; preparation of dilution; parasite exposure; response evaluation; data analysis; and reporting. Information from the presentations of the workshop, and also from other studies, allowed for the identification of procedures inside a given step that need to be standardized as they were...
Source: Journal of Fish Diseases - December 18, 2017 Category: Zoology Authors: S L Mar ín, J Mancilla, M A Hausdorf, D Bouchard, M S Tudor, F Kane Tags: SPECIAL ISSUE Source Type: research
Issue Information
(Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - December 15, 2017 Category: Zoology Tags: ISSUE INFORMATION Source Type: research
Cover Image
The cover image, by Å H Garseth et al., is based on the Original Article A survey of salmon gill poxvirus (SGPV) in wild salmonids in Norway, DOI: 10.1111/jfd.12688. Image Credit: Norwegian Veterinary Institute. (Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - December 15, 2017 Category: Zoology Authors: Å H Garseth, M C Gjessing, T Moldal, A G Gjevre Tags: COVER IMAGE Source Type: research
Vaccination of Atlantic lumpfish (Cyclopterus lumpus L.) at a low temperature leads to a low antibody response against Aeromonas salmonicida
Journal of Fish Diseases,Volume 41, Issue 4, Page 613-623, April 2018. (Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - December 11, 2017 Category: Zoology Source Type: research
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Journal of Fish Diseases,Volume 41, Issue 4, Page 613-623, April 2018. (Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - December 11, 2017 Category: Zoology Source Type: research
Detection and molecular characterization of betanodaviruses retrieved from bivalve molluscs
Journal of Fish Diseases,Volume 41, Issue 4, Page 603-611, April 2018. (Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - December 5, 2017 Category: Zoology Source Type: research
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Journal of Fish Diseases,Volume 41, Issue 4, Page 603-611, April 2018. (Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - December 5, 2017 Category: Zoology Source Type: research
Dermocystidium sp. infection in farmed hybrid fish Colossoma macropomum × Piaractus brachypomus in Brazil
(Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - December 1, 2017 Category: Zoology Authors: R Y Fujimoto, M V S Couto, N C Sousa, D G Diniz, J A P Diniz, R R Madi, M L Martins, J C Eiras Tags: SHORT COMMUNICATION Source Type: research
Vaccination of Atlantic lumpfish (Cyclopterus lumpus L.) at a low temperature leads to a low antibody response against Aeromonas salmonicida
We present a study on the effect of water temperature on immunization of Atlantic lumpfish. In total, 360 fish were vaccinated with either 50 μl of an oil‐based injection vaccine (VAX), with Aeromonas salmonicida and Vibrio salmonicida antigens, or PBS. Fish were vaccinated at three different water temperatures, 5°C, 10°C and 15°C, and sorted into six groups (N = 60). Lumpfish were weighed every 3 weeks after vaccination, sampled at 3, 6, 9 and 18 weeks post‐immunization (wpi) and evaluated by modified Speilberg score, ELISA and immunoblotting. Vaccinated fish showed low antibody response against V. salmonici...
Source: Journal of Fish Diseases - December 1, 2017 Category: Zoology Authors: T Erkinharju, R A Dalmo, Ø Vågsnes, I Hordvik, T Seternes Tags: ORIGINAL ARTICLE Source Type: research
Detection and molecular characterization of betanodaviruses retrieved from bivalve molluscs
Abstract
Betanodaviruses are small ssRNA viruses responsible for viral encephalopathy and retinopathy, otherwise known as viral nervous necrosis, in marine fish worldwide. These viruses can be either horizontally or vertically transmitted and have been sporadically detected in invertebrates, which seem to be one of the possible viral sources. Twenty‐eight new betanodavirus strains were retrieved in three molluscs species collected from different European countries between 2008 and 2015. The phylogenetic analyses revealed that strains retrieved from bivalve molluscs are closely related to viruses detected in finfish in So...
Source: Journal of Fish Diseases - December 1, 2017 Category: Zoology Authors: E Volpe, M Grodzki, V Panzarin, A Guercio, G Purpari, P Serratore, S Ciulli Tags: ORIGINAL ARTICLE Source Type: research
