Methods for identification and differentiation of different Shewanella spp. isolates for diagnostic use
In this study, 74 isolates of Shewanella spp. were analysed. For species identification, biochemical techniques, 16S rRNA sequencing, MALDI‐TOF MS and the Sherlock Microbial Identification System (MIS) based on the composition of fatty acid ethyl esters were compared. The phylogenetic relationship, cytotoxicity in vitro and resistance against antibiotics were tested. The most reliable method for species identification was 16S rRNA sequencing. From diseased fish, clinically healthy fish and the aquatic environment, different Shewanella species were isolated. This indicates that Shewanella spp. is widespread in the aquati...
Source: Journal of Fish Diseases - December 27, 2017 Category: Zoology Authors: V Jung ‐Schroers, A Jung, M Ryll, J Bauer, F Teitge, D Steinhagen Tags: ORIGINAL ARTICLE Source Type: research
Methods for identification and differentiation of different Shewanella spp. isolates for diagnostic use
Journal of Fish Diseases,Volume 41, Issue 4, Page 689-714, April 2018. (Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - December 27, 2017 Category: Zoology Source Type: research
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Journal of Fish Diseases,Volume 41, Issue 4, Page 689-714, April 2018. (Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - December 27, 2017 Category: Zoology Source Type: research
Outbreak of mass mortality of yearling groupers of Epinephelus (Perciformes, Serranidae) associated with the infection of a suspected new enteric Sphaerospora (Myxozoa: Myxosporea) species in South China Sea
Abstract
A suspected new enteric Sphaerospora species was believed to be directly associated with the mass mortality of yearling groupers of Epinephelus spp. in South China. The epizootic generally emerged from late September to late April of the following year. The infection prevalence and mortality rate were significantly negatively correlated with fish size. Clinical signs included anorexia, cachexia and extrusion of white pulp‐like substance from anus after gentle pressure on the abdomen. Upon necropsy, severe intestinal oedema, thin and transparent intestinal wall, swollen spleen, kidney and gall bladder could be ob...
Source: Journal of Fish Diseases - December 19, 2017 Category: Zoology Authors: X H Liu, L W Xu, D Luo, Y L Zhao, Q Q Zhang, G F Liu, J Y Zhang Tags: ORIGINAL ARTICLE Source Type: research
Purinergic signalling displays an anti ‐inflammatory profile in the spleen of fish experimentally infected with Aeromonas caviae: Modulation of the immune response
Journal of Fish Diseases,Volume 41, Issue 4, Page 683-687, April 2018. (Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - December 19, 2017 Category: Zoology Source Type: research
PCR procedure for detecting the fish pathogen Tenacibaculum dicentrarchi
Journal of Fish Diseases,Volume 41, Issue 4, Page 715-719, April 2018. (Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - December 19, 2017 Category: Zoology Source Type: research
Outbreak of mass mortality of yearling groupers of Epinephelus (Perciformes, Serranidae) associated with the infection of a suspected new enteric Sphaerospora (Myxozoa: Myxosporea) species in South China Sea
Journal of Fish Diseases,Volume 41, Issue 4, Page 663-672, April 2018. (Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - December 19, 2017 Category: Zoology Source Type: research
Bromodeoxyuridine DNA labelling reveals host and parasite proliferation in a fish ‐myxozoan model
Journal of Fish Diseases,Volume 41, Issue 4, Page 651-662, April 2018. (Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - December 19, 2017 Category: Zoology Source Type: research
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Journal of Fish Diseases,Volume 41, Issue 4, Page 683-687, April 2018. (Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - December 19, 2017 Category: Zoology Source Type: research
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Journal of Fish Diseases,Volume 41, Issue 4, Page 715-719, April 2018. (Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - December 19, 2017 Category: Zoology Source Type: research
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Journal of Fish Diseases,Volume 41, Issue 4, Page 663-672, April 2018. (Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - December 19, 2017 Category: Zoology Source Type: research
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Journal of Fish Diseases,Volume 41, Issue 4, Page 651-662, April 2018. (Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - December 19, 2017 Category: Zoology Source Type: research
PCR procedure for detecting the fish pathogen Tenacibaculum dicentrarchi
(Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - December 19, 2017 Category: Zoology Authors: R Avenda ño‐Herrera, R Irgang, D Tapia‐Cammas Tags: SHORT COMMUNICATION Source Type: research
Purinergic signalling displays an anti ‐inflammatory profile in the spleen of fish experimentally infected with Aeromonas caviae: Modulation of the immune response
Abstract
Extracellular adenosine triphosphate (ATP) and its metabolite adenosine (Ado) are recognized as key mediators of immune and inflammatory responses. Depending on its concentration, ATP may act as an immunostimulant or immunodepressant, while Ado levels display an anti‐inflammatory profile. The aim of this study was to evaluate whether splenic purinergic signalling is capable of modulating immune and inflammatory responses in fish experimentally infected with Aeromonas caviae. Triphosphate diphosphohydrolase (NTPDase) and 5′‐nucleotidase activities increased in the spleen of silver catfish (Rhamdia quelen) exp...
Source: Journal of Fish Diseases - December 19, 2017 Category: Zoology Authors: M D Baldissera, C F Souza, N B Bottari, C M Verdi, R C V Santos, B S Vizzotto, B Baldisserotto Tags: ORIGINAL ARTICLE Source Type: research
Bromodeoxyuridine DNA labelling reveals host and parasite proliferation in a fish ‐myxozoan model
Abstract
Enteromyxum leei is a myxozoan parasite responsible for enteritis in gilthead sea bream (Sparus aurata). The parasite proliferates in the paracellular space of the intestinal epithelium and induces an inflammatory reaction. To assess intestinal cell turnover and parasite proliferation, fish were infected with the parasite by anal intubation; after 17 and 64 days, the cell proliferative marker bromodeoxyuridine (BrdU) was administered; and after 24 hr, tissue samples were taken for immunohistochemical detection. Parasite exposure induced increased epithelial and immune cell proliferation in all intestinal segment...
Source: Journal of Fish Diseases - December 19, 2017 Category: Zoology Authors: I Estensoro, G P érez‐Cordón, A Sitjà‐Bobadilla, M C Piazzon Tags: ORIGINAL ARTICLE Source Type: research
