First isolation of Vibrio tapetis and an atypical strain of Aeromonas salmonicida from skin ulcerations in common dab (Limanda limanda) in the North Sea
Abstract Skin ulcerations rank amongst the most prevalent lesions affecting wild common dab (Limanda limanda) with an increase in prevalence of up to 3.5% in the Belgian part of the North Sea. A complex aetiology of these ulcerations is suspected, and many questions remain on the exact factors contributing to these lesions. To construct the aetiological spectrum of skin ulcerations in flatfish, a one‐day monitoring campaign was undertaken in the North Sea. Fifteen fish presented with one or more ulcerations on the pigmented and/or non‐pigmented side. Pathological features revealed various stages of ulcerations with los...
Source: Journal of Fish Diseases - November 17, 2017 Category: Zoology Authors: M Vercauteren, E De Swaef, A Declercq, L Bosseler, S Gulla, S Balboa, J L Romalde, L Devriese, H Polet, F Boyen, K Chiers, A Decostere Tags: ORIGINAL ARTICLE Source Type: research

Experimental infection by Yersinia ruckeri O1 biotype 2 induces brain lesions and neurological signs in rainbow trout (Oncorhynchus mykiss)
In conclusion, Y. ruckeri strain 07111224 causes encephalitis in the acute phase of infection, which could explain why Y. ruckeri‐affected fish show exophthalmia and erratic swimming known as signs of ERM. (Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - November 17, 2017 Category: Zoology Authors: H K Str øm, M Ohtani, B Nowak, T S Boutrup, B Jones, M K Raida, A M Bojesen Tags: ORIGINAL ARTICLE Source Type: research

Statistical modelling of sea lice count data from salmon farms in the Faroe Islands
This study is useful for understanding sea lice levels and treatments, and for in situ analysis of the sea‐lice development times and growth rates. (Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - November 17, 2017 Category: Zoology Authors: H Gislason Tags: SPECIAL ISSUE Source Type: research

Comparison of gene expression in post ‐smolt Atlantic salmon challenged by LF‐89‐like and EM‐90‐like Piscirickettsia salmonis isolates reveals differences in the immune response associated with pathogenicity
Abstract Piscirickettsiosis is the main bacterial disease affecting the Chilean salmon farming industry and is responsible for high economic losses. The aim of this study was to describe and comparatively quantify the immune response of post‐smolt Atlantic salmon infected by cohabitation with fish bearing LF‐89‐like and EM‐90‐like Piscirickettsia salmonis. The expression of 17 genes related to the immune response was studied in head kidney from cohabitant fish by RT‐qPCR. Our results at the transcriptomic level suggest that P. salmonis is able to manipulate the kinetics of cytokine production in a way that mig...
Source: Journal of Fish Diseases - November 16, 2017 Category: Zoology Authors: M Rozas ‐Serri, A Peña, G Arriagada, R Enríquez, L Maldonado Tags: ORIGINAL ARTICLE Source Type: research

Neoplasia in goldlined seabream, Rhabdosargus sarba (Forssk ål, 1775)
(Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - November 10, 2017 Category: Zoology Authors: V Singaravel, A Gopalakrishnan, K Raja, M Rajkumar, H W Ferguson Tags: SHORT COMMUNICATION Source Type: research

First analysis by MALDI ‐TOF MS technique of Chryseobacterium species relevant to aquaculture
(Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - November 10, 2017 Category: Zoology Authors: M P érez‐Sancho, A I Vela, M Kostrzewa, L Zamora, A Casamayor, L Domínguez, J F Fernández‐Garayzábal Tags: SHORT COMMUNICATION Source Type: research

Corrigendum
(Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - November 8, 2017 Category: Zoology Tags: CORRIGENDUM Source Type: research

Establishment of a cell line from the kidney of black carp and its susceptibility to spring viremia of carp virus
In this study, we established and characterized a cell line derived from the kidney of black carp (Mylopharyngodon piceus), which is an important freshwater aquaculture species. The cell line was designated as MPK and subcultured for more than 70 passages in DMEM medium containing 10% fetal bovine serum (FBS) at 28°C. MPK had a modal diploid chromosome number of 48. Moreover, a transient MPK transfection efficiency was up to 18% using a green fluorescent protein plasmid by a modified electroporation. In addition, the MPK cells showed susceptibility to spring viremia of carp virus (SVCV), as demonstrated by the presence of...
Source: Journal of Fish Diseases - October 25, 2017 Category: Zoology Authors: T Xue, Y Z Wang, Q H Pan, Q Wang, J F Yuan, T S Chen Tags: ORIGINAL ARTICLE Source Type: research

CyHV ‐2 transmission in traded goldfish stocks in Germany—A case study
(Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - October 25, 2017 Category: Zoology Authors: M Adamek, J Hellmann, V Jung ‐Schroers, F Teitge, D Steinhagen Tags: SHORT COMMUNICATION Source Type: research

Carp edema virus from three genogroups is present in common carp in Hungary
Abstract Hungary is an important carp producer with intensive trading relationships with farms in other carp‐producing areas in Europe. Carp in Europe were recently found infected with carp edema virus (CEV), a poxvirus which causes the koi sleepy disease (KSD) syndrome. Moribund carp were collected from 17 fish farms and angling ponds in different regions of Hungary. Histological analysis of gills from these carp revealed a proliferation of the interlamellar epithelium and an infiltration by eosinophilic cells. In 13 of 17 of these carp, CEV DNA was detected by qPCR and in seven fish more than 1 × 104 copies of virus...
Source: Journal of Fish Diseases - October 24, 2017 Category: Zoology Authors: M Adamek, F Baska, B Vincze, D Steinhagen Tags: ORIGINAL ARTICLE Source Type: research

Histopathological and ultrastructural assessment of two mugilid species infected with myxozoans and helminths
Abstract The histopathology and ultrastructure of the intestine of mullets, Liza ramada and Liza saliens, from Comacchio lagoons (northern Italy) naturally infected with myxozoans and helminths were investigated and described. Sixty‐two (80.5%) of 77 mullets harboured one or more of the following parasites species: Myxobolus mugchelo (Myxozoa), Neoechinorhynchus agilis (Acanthocephala), Haplosplanchnus pachysomus and Dicrogaster contractus (Digenea). Co‐occurrence of helminths with myxozoans was common. The main damage caused by digeneans was destruction of the mucosal epithelium of the villi, necrosis and degeneration...
Source: Journal of Fish Diseases - October 24, 2017 Category: Zoology Authors: B Sayyaf Dezfuli, G Castaldelli, L Giari Tags: ORIGINAL ARTICLE Source Type: research

Genetic parameters for resistance against Flavobacterium columnare in Nile tilapia Oreochromis niloticus (Linnaeus, 1758)
This study reveals the potential of selective breeding to increase disease resistance to F. columnare in the studied population of Nile tilapia. (Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - October 24, 2017 Category: Zoology Authors: P Wonmongkol, S Sukhavachana, K Ampolsak, P Srisapoome, T Suwanasopee, S Poompuang Tags: ORIGINAL ARTICLE Source Type: research

Antimicrobial susceptibility of Flavobacterium psychrophilum isolates from the United Kingdom
In this study, the antimicrobial susceptibilities of 133 F. psychrophilum isolates, 118 of which were from the UK, were evaluated by broth microdilution and disc diffusion methods following VET04‐A2 and VET03‐A guidelines of Clinical and Laboratory Standards Institute (CLSI), respectively. Isolates were categorized as wild type (fully susceptible, WT) or non‐wild type (NWT) using normalized resistance interpretation (NRI)‐determined cut‐off values (COWT). Broth microdilution testing showed that only 12% of UK isolates were WT to oxolinic acid (MIC COWT ≤ 0.25 mg/L) and 42% were WT for oxytetracycline (MIC C...
Source: Journal of Fish Diseases - October 24, 2017 Category: Zoology Authors: T P H Ngo, P Smith, K L Bartie, T D Kim, D W Verner ‐Jeffreys, R Hoare, A Adams Tags: ORIGINAL ARTICLE Source Type: research

DNA vaccines for fish: Review and perspectives on correlates of protection
Abstract Recently in 2016, the European Medicines Agency (EMA) recommended granting a marketing authorization in the EU for “Clynav,” a DNA vaccine against salmon pancreas disease (salmonid alphavirus‐3). Generally, DNA vaccines induce both early and late immune responses in fish that may be protective against disease. Several transcriptomic approaches have been performed to map immunome profiles following DNA vaccination, but the precise immune mechanism(s) that is responsible for protection is not known, although reasonable suggestions have been made. The current review includes an overview on main transcriptomic f...
Source: Journal of Fish Diseases - October 24, 2017 Category: Zoology Authors: R A Dalmo Tags: REVIEW Source Type: research

A rapid method for simultaneously diagnosing four shrimp diseases using PCR ‐DNA chromatography method
(Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - October 24, 2017 Category: Zoology Authors: K Koiwai, T Kodera, J Thawonsuwan, M Kawase, H Kondo, I Hirono Tags: SHORT COMMUNICATION Source Type: research