Risk factors and long-term health consequences of macrosomia: a prospective study in Jiangsu Province, China
Publication date: July 2012 Source:Journal of Biomedical Research, Volume 26, Issue 4 Author(s): Shouyong Gu , Xiaofei An , Liang Fang , Xiaomin Zhang , Chunyan Zhang , Jingling Wang , Qilan Liu , Yanfang Zhang , Yongyue Wei , Zhibin Hu , Feng Chen , Hongbing Shen We sought to determine risk factors associated with fetal macrosomia and to explore the long-term consequence of infant macrosomia at the age of 7 years. A prospective population based cohort study was designed to examine the associations between maternal and perinatal characteristics and the risk of macrosomia. A nested case-control study was con...
Source: Journal of Biomedical Research - November 3, 2014 Category: Biochemistry Source Type: research
MiRNA-429 suppresses the growth of gastric cancer cells in vitro
This study was aimed to investigate the regulation of miRNA-429 (miR-429) in gastric cancer cells on cell proliferation and apoptosis. Quantitative PCR was employed to detect the expressions of miR-429 after eukaryotic expression plasmid of miR-429 and its inhibitor were transiently transfected into poorly differentiated human gastric cancer cell line BGC823. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction as-says were used to examine proliferation ability. Apoptosis was analyzed by flow cytometry after transfection. The results showed that 48 h after transfection, overexpression of miR-429...
Source: Journal of Biomedical Research - November 3, 2014 Category: Biochemistry Source Type: research
Overexpression of the mTERT gene by adenoviral vectors promotes the proliferation of neuronal stem cells in vitro and stimulates neurogenesis in the hippocampus of mice
Publication date: September 2012 Source:Journal of Biomedical Research, Volume 26, Issue 5 Author(s): Mengying Liu , Yao Hu , Lijuan Zhu , Chen Chen , Yu Zhang , Weixiang Sun , Qigang Zhou We sought to construct the adenoviral vector carrying the gene encoding mouse telomerase reverse transcriptase (mTERT), as well as detect its expression and effect on the proliferation of neuronal stem cells. mTERT was amplified by RT-PCR and then the eukaryotic expression vector of pDC-EGFP-TERT was constructed. After DNA sequence analysis, we detected that there were 293 cells transfected with pDC-EGFP-TERT and helper adenov...
Source: Journal of Biomedical Research - November 3, 2014 Category: Biochemistry Source Type: research
Cyclosporine A stimulated hair growth from mouse vibrissae follicles in an organ culture model
Publication date: September 2012 Source:Journal of Biomedical Research, Volume 26, Issue 5 Author(s): Wenrong Xu , Weixin Fan , Kun Yao Hypertrichosis is one of the most common side effects of systemic cyclosporine A therapy. It has been previously shown that cyclosporine A induces anagen and inhibits catagen development in mice. In the present study, to explore the mechanisms of cyclosporine A, we investigated the effects of cyclosporine A on hair shaft elongation, hair follicle cell proliferation, apoptosis, and mRNA expression of selected growth factors using an organ culture model of mouse vibrissae. In this mod...
Source: Journal of Biomedical Research - November 3, 2014 Category: Biochemistry Source Type: research
Corrigendum
Publication date: September 2012 Source:Journal of Biomedical Research, Volume 26, Issue 5 (Source: Journal of Biomedical Research)
Source: Journal of Biomedical Research - November 3, 2014 Category: Biochemistry Source Type: research
Hydrogen – rich saline protects against ultraviolet B radiation injury in rats
In this study, we sought to demonstrate that hydrogen-rich saline has the effect on skin injuries caused by UVB radiation. UVB radiation was irradiated on female C57BL/6 rats to induce skin injury. Hydrogen-rich saline and nitrogen-rich saline were administered to rats by intraperitoneal injection. Skin damage was detected by microscope after injury. UVB radiation had a significant affection in tumor necrosis factor alpha, interleukin (IL)-1β and IL-6 levels, tissue superox-ide dismutase, malondialdehyde and nitric oxide activity. Hydrogen-rich saline had a protective effect by altering the levels of these markers and rel...
Source: Journal of Biomedical Research - November 3, 2014 Category: Biochemistry Source Type: research
X-ray irradiation selectively kills thymocytes of different stages and impairs the maturation of donor – derived CD4 +CD8 + thymocytes in recipient thymus
Publication date: September 2012 Source:Journal of Biomedical Research, Volume 26, Issue 5 Author(s): Jinbo Li , Hongquan Cai , Jianliang Jin , Qian Wang , Dengshun Miao The aim of the present study was to determine whether the sensitivity of thymocytes to X-ray radiation depends on their proliferative states and whether radiation impairs the maturation of donor-derived thymocytes in recipient thymus. We assigned 8-week-old C57BL/6J mice into three treatment groups: 1) untreated; 2) X-ray radiation; 3) X-ray radiation plus bone marrow transplantation with donor bone marrow cells from transgenic mice expressing enh...
Source: Journal of Biomedical Research - November 3, 2014 Category: Biochemistry Source Type: research
Glycine attenuates myocardial ischemia – reperfusion injury by inhibiting myocardial apoptosis in rats
Publication date: September 2012 Source:Journal of Biomedical Research, Volume 26, Issue 5 Author(s): Xiaozheng Zhong , Xiaoyu Li , Lingling Qian , Yiming Xu , Yan Lu , Jing Zhang , Nan Li , Xudong Zhu , Jingjing Ben , Qing Yang , Qi Chen Glycine is a well-documented cytoprotective agent. However, whether it has a protective effect against myocardial ischemia-reperfusion injury in vivo is still unknown. By using an open-chest anesthetized rat model, we found that glycine reduced the infarct size by 21% in ischemia-reperfusion injury rats compared with that in the vehicle-treated MI/R rats. The left ventricul...
Source: Journal of Biomedical Research - November 3, 2014 Category: Biochemistry Source Type: research
Sox2 enhances the tumorigenicity and chemoresistance of cancer stem-like cells derived from gastric cancer
Publication date: September 2012 Source:Journal of Biomedical Research, Volume 26, Issue 5 Author(s): Tian Tian , Yajie Zhang , Shouyu Wang , Jianwei Zhou , Shan Xu Gastric cancer stem-like cells (GCSCs) have been identified to possess the ability of self-renewal and tumor initiation. However, the mechanisms involved remain largely unknown. Here, we isolated and characterized the GCSCs by side population (SP) sorting procedure and cultured sphere cells (SC) from human gastric cancer cell lines SGC-7901, BGC-823, MGC-803, HGC-27 and MKN-28. The sorting and culture assay revealed that SP cells proliferated in an asy...
Source: Journal of Biomedical Research - November 3, 2014 Category: Biochemistry Source Type: research
STAT3 mediates resistance of CD44+CD24−/low breast cancer stem cells to tamoxifen in vitro
Publication date: September 2012 Source:Journal of Biomedical Research, Volume 26, Issue 5 Author(s): Xiaoyan Wang , Guozhu Wang , Yi Zhao , Xiaoan Liu , Qiang Ding , Jingping Shi , Yin Ding , Shui Wang We sought to determine whether STAT3 mediated tamoxifen resistance of breast cancer stem cells in vitro. The capacities for mammosphere formation and STAT3 expression of CD44+CD24−/low MCF-7 and MCF-7 were observed. The CD44+CD24−/low subpopulation ratio and its sensitivity to adriamycin were analyzed in MCF-7 and TAM resistant (TAM-R) cells. Cell cycle, apoptosis, STAT3 and phospho-STAT3 changes were observ...
Source: Journal of Biomedical Research - November 3, 2014 Category: Biochemistry Source Type: research
The role of aberrant promoter hypermethylation of DACT1 in bladder urothelial carcinoma
Publication date: September 2012 Source:Journal of Biomedical Research, Volume 26, Issue 5 Author(s): Huan Cheng , Zhonglei Deng , Zengjun Wang , Wei Zhang , Jiantang Su The purpose of this study was to determine the relationship between hypermethylation of DACT1 gene promoter and lower mRNA expression in bladder urothelial carcinoma tissue. The methylation status of 29 urothelial carcinoma samples and 29 normal tissue samples were examined by methylation-specific polymerase chain reaction (MSP). The DACT1 mRNA transcript levels and DACT1 protein levels in all samples were then evaluated to define the relationship...
Source: Journal of Biomedical Research - November 3, 2014 Category: Biochemistry Source Type: research
A germline variant N375S in MET and gastric cancer susceptibility in a Chinese population
In this study, we performed a case-control study to assess the association between N375S and gastric cancer risk in 1,681 gastric cancer cases and 1,858 cancer-free controls. Logistic regression analysis was applied to estimate crude and adjusted odds ratios (ORs) and 95% confidence intervals (CIs) for the associations between genotypes and gastric cancer risk. We found that MET N375S variant genotypes (NS/SS) were associated with a significantly decreased risk of gastric cancer (OR = 0.78, 95% CI = 0.63-0.96, P = 0.021) compared with the wildtype homozygote (NN). The finding indicates that this germline variant in MET may...
Source: Journal of Biomedical Research - November 3, 2014 Category: Biochemistry Source Type: research
Growth hormone receptor expression in human primary gastric adenocarcinoma
Publication date: September 2012 Source:Journal of Biomedical Research, Volume 26, Issue 5 Author(s): Xiaodong Yang , Ping Huang , Feng Wang , Zekuan Xu , Xiaonin Wang The aim of this study was to determine the expression of growth hormone receptor (GHR) in patients with primary gastric adenocarcinoma. We investigated 48 specimens of primary gastric adenocarcinoma and their corresponding normal gastric mucosa. Immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) were used to detect the expression of GHR. Immunohistochemical analyses revealed that GHR was expressed in human primary gast...
Source: Journal of Biomedical Research - November 3, 2014 Category: Biochemistry Source Type: research
Favre-Racouchot syndrome associated with eyelid papilloma: a case report
Publication date: November 2012 Source:Journal of Biomedical Research, Volume 26, Issue 6 Author(s): Ruzhi Zhang , Wenyuan Zhu A 55-year-old Chinese man presented with an asymptomatic pedunculated elevation on his left lower eyelid which had been gradually increasing in size during the past three years. The patient was diagnosed with eyelid papilloma by pathological examination. Concomitantly, the patient developed open comedones with a bilateral linear distribution, along with oblique wrinkle lines in his infraorbital regions. These lesions were noninflammatory and remained unchanged for two years. To the best of ou...
Source: Journal of Biomedical Research - November 3, 2014 Category: Biochemistry Source Type: research
Generation and characterization of an anti-GP73 monoclonal antibody for immunoblotting and sandwich ELISA
Publication date: November 2012 Source:Journal of Biomedical Research, Volume 26, Issue 6 Author(s): Aixia Zhang , Brian Cao Recently, serum Golgi protein 73 (GP73) levels have been found to be elevated in patients with hepatocellular carcinoma (HCC), and GP73 has been proposed as a novel marker for HCC. However, GP73 levels in patients remain controversial due to the specificity of the anti-GP73 antibody-based enzyme linked immunosorbent assay (ELISA). Therefore, an anti-GP73 antibody with high specificity was highly demanded. In the present study, by hybridoma screening, we generated an anti-GP73 monoclonal antibod...
Source: Journal of Biomedical Research - November 3, 2014 Category: Biochemistry Source Type: research
