X-ray irradiation selectively kills thymocytes of different stages and impairs the maturation of donor – derived CD4 +CD8 + thymocytes in recipient thymus

Publication date: September 2012 Source:Journal of Biomedical Research, Volume 26, Issue 5 Author(s): Jinbo Li , Hongquan Cai , Jianliang Jin , Qian Wang , Dengshun Miao The aim of the present study was to determine whether the sensitivity of thymocytes to X-ray radiation depends on their proliferative states and whether radiation impairs the maturation of donor-derived thymocytes in recipient thymus. We assigned 8-week-old C57BL/6J mice into three treatment groups: 1) untreated; 2) X-ray radiation; 3) X-ray radiation plus bone marrow transplantation with donor bone marrow cells from transgenic mice expressing enhanced green fluorescent protein (GFP) on a universal promoter. After 4 weeks, the size of the thymus, the number and proliferation of thymocytes and ratios of different stage thymocytes were analyzed by immunohisto-chemistry and flow cytometry. The results showed that: 1) CD4+CD8+ thymocytes were more sensitive to X-ray radiation-induced cell death than other thymocytes; 2) the proliferative capacity of CD4+CD8+ thymocytes was higher than that of other thymocytes; 3) the size of the thymus, the number of thymocytes and ratios of thymo-cytes of different stages in irradiated mice recovered to the normal level of untreated mice by bone marrow trans-plantation; 4) the ratio of GFP-positive CD4+CD8+ thymocytes increased significantly, whereas the ratio of GFP-positive CD4+ or CD8+ thymocytes decreased significantly. These results indicate that the degree of sens...
Source: Journal of Biomedical Research - Category: Biochemistry Source Type: research