Purification and properties of beta ‐cyclomaltodextrin glucanotransferase from Bacillus flexus SV 1
Cyclomaltodextrin glucanotransferase is a unique enzyme that degrades starch into cyclic oligosaccharides called cyclodextrins, which have numerous applications in various industries such as pharmaceutical, textile, agricultural, cosmetics etc. Due to its wide applications, microorganism producing one type of cyclodextrin is of interest as it simplifies the down streaming process of separating mixture of cyclodextrins. In the present study, β‐CGTase was isolated from Bacillus flexus SV 1 and biochemically characterized. Enzyme was purified by starch adsorption followed by DEAE cellulose column chromatography which resul...
Source: Journal of Basic Microbiology - August 1, 2017 Category: Microbiology Authors: Shwetha V. Reddy, Sunil S. More, Goutham S. Annappa Tags: RESEARCH PAPER Source Type: research
Complete degradation of dimethyl phthalate by a Comamonas testosterone strain
A Comamonas testosterone bacterial strain, named as DB‐7, capable of utilizing dimethyl phthalate (DMP) as sole carbon source and energy for growth was isolated from soil with plastic film mulching by an enrichment culture technique. This bacterium was identified as C. testosterone by 16S rRNA sequence analysis and phospholipid fatty acid profile. DB‐7 could degrade more than 99% of 450 mg L−1 DMP within 14 hours, and degraded DMP of different concentrations rapidly. The optimal degradation temperature and pH were 30–35 °C and pH 9.0, respectively. The degradation rate of DMP was positively related to inocul...
Source: Journal of Basic Microbiology - August 1, 2017 Category: Microbiology Authors: Jing Li, Feng Luo, Dian Chu, Huanling Xuan, Xianzhu Dai Tags: RESEARCH PAPER Source Type: research
The cadmium tolerance in Saccharomyces cerevisiae depends on inorganic polyphosphate
The sensitivity to cadmium (Cd(II)), an important environmental pollutant, was studied in the cells of Saccharomyces cerevisiae strains with genetically altered polyphosphate metabolism. The strains overproducing polyphosphatases PPX1 or PPN1 were more sensitive to Cd(II) than the parent strain. The half maximal inhibitory concentrations were 0.02 and 0.05 mM for the transformants and the parent strain, respectively. Transformant strains cultivated in the presence of Cd(II) show a decrease in the content of short‐chained cytosolic acid soluble polyphosphate. The role of this polyphosphate fraction in detoxification of ...
Source: Journal of Basic Microbiology - August 1, 2017 Category: Microbiology Authors: Ludmila Trilisenko, Ekaterina Kulakovskaya, Tatiana Kulakovskaya Tags: RESEARCH PAPER Source Type: research
Metagenomic alkaline protease from mangrove sediment
In this study, we identified a protease‐producing clone from a metagenomic library derived from mangrove sediment. The protease was purified by ammonium sulphate precipitation and gel filtration chromatography, with a yield of 77.27% and a specific activity of 8.57 U μg−1. It had a molecular weight of approximately 70 kDa. MS/MS in ESI‐Q‐TOF revealed nine peptides similar to a peptidase of Bacillus safensis. The aligned partial sequence showed 47.48% identity and 82.74% similarity to the conserved domains of a glutamyl aminopeptidase from the human gut metagenome and 32.12% total coverage. The protease had a...
Source: Journal of Basic Microbiology - August 1, 2017 Category: Microbiology Authors: Tharcilla B. A. Pessoa, Rachel P. Rezende, Eric de Lima Silva Marques, Carlos P. Pirovani, Thalis F. dos Santos, Ana C. dos Santos Gon çalves, Carla C. Romano, Natielle C. Dotivo, Ana C. O. Freitas, Luiz C. Salay, João C. T. Dias Tags: RESEARCH PAPER Source Type: research
Indigenously associated methanogens intensified the metabolism in hydrogenosomes of anaerobic fungi with xylose as substrate
In conclusion, the co‐culture with methanogens enhanced “energy yields” of anaerobic fungi by removing the accumulated formate, decreased the metabolism in cytosol, for example, the lactate pathway, and increased the metabolism in hydrogenosomes, for example, the acetate pathway. (Source: Journal of Basic Microbiology)
Source: Journal of Basic Microbiology - August 1, 2017 Category: Microbiology Authors: Yuanfei Li, Wei Jin, Chunlong Mu, Yanfen Cheng, Weiyun Zhu Tags: RESEARCH PAPER Source Type: research
Nitrogen fixing potential of various heterotrophic Bacillus strains from a tropical estuary and adjacent coastal regions
In the present study, we report the nitrogen fixing potential of heterotrophic diazotrophs isolated from a tropical estuary and adjacent coastal sea. Results of the study revealed that most of the species that are capable of fixing nitrogen in the study area belongs to the genus Bacillus. The isolates from the estuary showed maximum homology with Bacillus megaterium, B. cereus, B. safencis, B. licheniformis, B. aerophilus, B. oceanisediminis, B. flexus, B. aquimaris, B. vietnamensis, and B. subterraneaus, whereas the diazotrophic isolates from coastal samples were closely related to B. subtilis, B. megaterium, B. circulans...
Source: Journal of Basic Microbiology - August 1, 2017 Category: Microbiology Authors: Jesmi Yousuf, Jabir Thajudeen, Mujeeb Rahiman, Soumya Krishnankutty, Aneesa P. Alikunj, Mohamed H. A. Abdulla Tags: RESEARCH PAPER Source Type: research
Salmonella Choleraesuis outer membrane vesicles: Proteomics and immunogenicity
In this study, we purified OMVs released by S. Choleraesuis strain χ3545 and characterized their lipopolysaccharide (LPS) profile. The OMVs contained intact LPS molecules. By using LC‐MS/MS, we identified 192 proteins in the OMVs. In addition, the subcellular location and biological functions of the vesicles was predicted. The proteins were mainly derived from outer membranes and cytoplasm. Several proteins were immunoreactive and associated with the secretion pathway. Some putative multi‐drug resistance‐associated proteins were also identified. Furthermore, immunization experiment via intranasal or intraperitoneal ...
Source: Journal of Basic Microbiology - July 26, 2017 Category: Microbiology Authors: Qiong Liu, Jie Yi, Kang Liang, Xiangmin Zhang, Qing Liu Tags: RESEARCH PAPER Source Type: research
Genetic diversity of catechol 1,2 ‐dioxygenase in the fecal microbial metagenome
This study is the first to report the molecular and biochemical characterizations of a cold‐adapted catechol 1,2‐dioxygenase from a fecal microbial metagenome. (Source: Journal of Basic Microbiology)
Source: Journal of Basic Microbiology - July 26, 2017 Category: Microbiology Authors: Bo Xu, Caiyun Xiong, Meng Deng, Junjun Li, Xianghua Tang, Qian Wu, Junpei Zhou, Yunjuan Yang, Junmei Ding, Nanyu Han, Zunxi Huang Tags: RESEARCH PAPER Source Type: research
DnrI of Streptomyces peucetius binds to the resistance genes, drrAB and drrC but is activated by daunorubicin
The master regulator, DnrI of Streptomyces peucetius is a member of the family of transcriptional activator, Streptomyces antibiotic regulatory proteins (SARP), which controls the biosynthesis of antitumor anthracycline, daunorubicin (DNR) and doxorubicin (DXR). The binding of DnrI to the heptameric repeat sequence found within the −35 promoter region of biosynthetic gene, dpsE activates it. To combat the increased level of intracellular DNR, the cell has developed self resistance mechanism mediated by drrAB and drrC genes which are regulated by regulatory genes. We find that a drug non‐producing mutant, ΔdpsA, showed...
Source: Journal of Basic Microbiology - July 26, 2017 Category: Microbiology Authors: Francis Prija, Padmanabhan Srinivasan, Subhadeep Das, Karuppasamy Kattusamy, Ranjan Prasad Tags: RESEARCH PAPER Source Type: research
Antibacterial activity of fusion from biosynthesized acidocin/silver nanoparticles and its application for eggshell decontamination
Bacteriocins from lactic acid bacteria (LAB) are useful to control the persistent development of pathogenic microorganisms in food and medicine fields. The bacteriocin acidocin was extracted from Lactobacillus acidophilus M1 that was isolated from fermented milk, purified using ammonium sulphate fractionation, and gel filtration column chromatography using Sephadex matrix and applied as a potential antibacterial agent. The molecular weight of the purified acidocin was estimated using SDS–PAGE to be 6.6 kDa. The acidocin was compared with silver nanoparticles (SNPs), biosynthesized by Aspergillus brasiliensis (niger) AT...
Source: Journal of Basic Microbiology - July 24, 2017 Category: Microbiology Authors: A.B. Abeer Mohammed, Mahmoud A. Al ‐Saman, Ahmed A. Tayel Tags: RESEARCH PAPER Source Type: research
Ecological guild and enzyme activities of rhizosphere soil microbial communities associated with Bt ‐maize cultivation under field conditions in North West Province of South Africa
This study investigated the structure and enzymatic activity of rhizosphere soil microbial communities associated with field grown Bt and non‐Bt maize. Rhizosphere soil samples were collected from Bt and non‐Bt fields under dryland and irrigated conditions. Samples were subjected to chemical tests, enzyme analyses, and next generation sequencing. Results showed that nitrate and phosphorus concentrations were significantly higher in non‐Bt maize dryland soils, while organic carbon was significantly higher in non‐Bt maize irrigated field soil. Acid phosphatase and β‐glucosidase activities were significantly reduce...
Source: Journal of Basic Microbiology - July 21, 2017 Category: Microbiology Authors: Deidr é A. B. van Wyk, Rasheed Adeleke, Owen H. J. Rhode, Carlos C. Bezuidenhout, Charlotte Mienie Tags: RESEARCH PAPER Source Type: research
Issue Information: Journal of Basic Microbiology. 7/2017
(Source: Journal of Basic Microbiology)
Source: Journal of Basic Microbiology - July 3, 2017 Category: Microbiology Tags: Issue Information Source Type: research
Cover: Journal of Basic Microbiology. 7/2017
Cover illustration:
Trichoderma based biofilm with Azotobacter chroococcum.
(Photo: Kulandaivelu Velmourogane, Division of Microbiology, ICAR‐Indian Agricultural Research Institute, New Delhi, India) (Source: Journal of Basic Microbiology)
Source: Journal of Basic Microbiology - July 3, 2017 Category: Microbiology Tags: Cover Picture Source Type: research
Silver nanoparticles from Pilimelia columellifera subsp. pallida SL19 strain demonstrated antifungal activity against fungi causing superficial mycoses
In this study, we present the in vitro antifungal activity of silver nanoparticles (AgNPs) synthesized from acidophilic actinobacterium Pilimelia columellifera subsp. pallida SL19 strain, alone and in combination with antibiotics viz., amphotericin B, fluconazole, and ketoconazole against pathogenic fungi, namely Candida albicans, Malassezia furfur, and Trichophyton erinacei. The minimum inhibitory concentration (MIC) and minimum biocidal concentration (MBC) of AgNPs against test fungi were evaluated. The fractional inhibitory concentration (FIC) index was determined to estimate antifungal activity of AgNPs combined with a...
Source: Journal of Basic Microbiology - July 3, 2017 Category: Microbiology Authors: Magdalena Wypij, Joanna Czarnecka, Hanna Dahm, Mahendra Rai, Patrycja Golinska Tags: RESEARCH PAPER Source Type: research
Xylanase production from marine derived Trichoderma pleuroticola 08 ÇK001 strain isolated from Mediterranean coastal sediments
This study demonstrates that marine‐derived fungal strains are prolific sources for xylanase production and presents the first report about the production and characterization of xylanase from a marine derived T. pleuroticola strain. The characteristics of T. pleuroticola 08ÇK001 xylanase activity indicate possible employment in some industrial processes such as animal feed, juice and wine industries or paper pulping applications. (Source: Journal of Basic Microbiology)
Source: Journal of Basic Microbiology - July 1, 2017 Category: Microbiology Authors: Melih N. Korkmaz, Sennur C. Ozdemir, Ata ç Uzel Tags: RESEARCH PAPER Source Type: research
