Biocontrol ability of killer yeasts (Saccharomyces cerevisiae) isolated from wine against Colletotrichum gloeosporioides on grape
A total of 216 killer yeasts Saccharomyces cerevisiae, isolated from wine, were evaluated in controlling Colletotrichum gloeosporioides, a pre‐harvest anthracnose agent of grape. Three of these yeast isolates were tested positive for antagonizing C. gloeosporioides and were further evaluated for their mechanisms as biological control agents (BCAs): production of antifungal compounds, production of hydrolytic enzymes, inhibition of C. gloeosporioides conidia germination, colonization on grape berry, and efficiency in controlling anthracnose of grape. The results showed that all three S. cerevisiae isolates produced antifu...
Source: Journal of Basic Microbiology - November 6, 2017 Category: Microbiology Authors: Zongling Liu, Shuang Du, Yi Ren, Yanlin Liu Tags: RESEARCH PAPER Source Type: research
Characterization of a robust serine protease from Bacillus subtilis K ‐1
Process apt microbial proteases due to their wide range industrial applications have become the focus of intense scientific research during recent years. Considering the hostile process milieu, the proteases intended for application must be robust enough to withstand the extremes of temperature and pH, and presence of organic solvents and other potential enzyme inhibitors. Current study presents the characterization of a robust protease from a previously isolated bacterium Bacillus subtilis K‐1 (BSK‐1). Purification of BSK‐1 protease (5.21‐fold) was achieved to homogeneity by salt (ammonium sulfate) precipitation, ...
Source: Journal of Basic Microbiology - November 6, 2017 Category: Microbiology Authors: Satbir Singh, Puneet Gupta, Bijender K. Bajaj Tags: RESEARCH PAPER Source Type: research
Influence of fertilizers and rice cultivation methods on the abundance and diversity of phyllosphere microbiome
Rice paddies are man‐made, cross‐over ecologies of aquatic and terrestrial systems, which favor the proliferation of characteristic microbial communities. Moisture regimes under flooded and different levels of irrigation such as in direct seeded rice (DSR) and system of rice intensification (SRI) lead to modulation in crop physiology, soil nutrient availability, and the soil microbiome. However, the diversity of the rice phyllosphere microbiome is less investigated in terms of the influence of fertilizer application and the method of rice cultivation (conventional‐flooded, DSR and SRI). Scanning electron micrographs ...
Source: Journal of Basic Microbiology - November 1, 2017 Category: Microbiology Authors: Shobit Thapa, Kunal Ranjan, Balasubramanian Ramakrishnan, Kulandaivelu Velmourougane, Radha Prasanna Tags: RESEARCH PAPER Source Type: research
The DNA ‐methyltransferase inhibitor 5‐aza‐2‐deoxycytidine affects Humicola grisea enzyme activities and the glucose‐mediated gene repression
Humicola grisea var. thermoidea (Hgvt) is a thermophilic ascomycete that produces lignocellulolytic enzymes and it is proposed for the conversion of agricultural residues into useful byproducts. Drugs that inhibit the DNA methyltransferases (DNMTs) activity are employed in epigenetic studies but nothing is known about a possible effect on the production of fungal enzymes. We evaluated the effect of 5‐aza‐2′‐deoxycytidine (5‐Aza; a chemical inhibitor of DNMTs activity) on the secreted enzyme activity and on the transcription of cellulase and xylanase genes from Hgvt grown in agricultural residues and in glucose. U...
Source: Journal of Basic Microbiology - November 1, 2017 Category: Microbiology Authors: Jo ão Heitor C. Manfrão‐Netto, Thiago M. Mello‐de‐Sousa, Astrid R. Mach‐Aigner, Robert L. Mach, Marcio J. Poças‐Fonseca Tags: RESEARCH PAPER Source Type: research
Role of two 5 ‐aminolevulinic acid biosynthetic pathways in heme and secondary metabolite biosynthesis in Amycolatopsis orientalis
Analysis of the Amycolatopsis orientalis genome revealed that two genes, hemA1 and hemA2, belonging to divergent pathways, were involved in the biosynthesis of 5‐aminolevulinic acid. The roles of hemA1 and hemA2 were elucidated via genetic manipulation and metabolite analysis. The disruption of hemA1, encoding the glutamyl‐tRNAGlu reductase of the C5 pathway, was essential for cell growth and is used for heme synthesis. Overexpression of hemA1 resulted in elevated vancomycin and ECO‐0501 production in Amycolatopsis orientalis, and it was also effective in increasing the production of daptomycin and natamycin in other...
Source: Journal of Basic Microbiology - November 1, 2017 Category: Microbiology Authors: Li Zhu, Xiuping Qian, Daijie Chen, Mei Ge Tags: RESEARCH PAPER Source Type: research
Comparative transcriptome analysis of the calcium signaling and expression analysis of sodium/calcium exchanger in Aspergillus cristatus
Aspergillus cristatus develops into various stages under different Na concentrations: the sexual stage in 0.5 M NaCl and asexual development stage in 3 M NaCl. In order to explore whether the Ca2+ signaling pathway in A. cristatus responded to the changes in the salt stress, we analyzed the gene expression levels in A. cristatus respectively cultured in 0.5 M NaCl and 3 M NaCl. According to the BLAST analysis results, we identified 25 Ca2+‐signaling proteins in A. cristatus. The expression levels of most genes involved in the Ca2+‐signaling pathway in A. cristatus cultured in different salt concentrations showe...
Source: Journal of Basic Microbiology - November 1, 2017 Category: Microbiology Authors: Xiu ‐xiu Ren, Yuchen Wang, YongXiang Liu, Yumei Tan, Chunguang Ren, Yongyi Ge, Zuoyi Liu Tags: RESEARCH PAPER Source Type: research
Isolation and characterization of high protein and phycocyanin producing mutants of Arthrospira platensis
Cyanobacteria are known to exhibit their efficiency in producing high concentrations of compounds of commercial value. Arthrospira is one such cyanobacterium which is considered as important source of protein (65%) and other nutrients. In present study, chemical mutagenesis using N‐methyl‐Ń‐Nitro‐nitrosoguanidine (NTG), a proven potent mutagen for cyanobacteria was used to bring stable and desirable alteration in Arthrospira platensis ARM 730. Three morphological mutants (G‐1, G‐2, and SF) were selected and characterized. The G‐1 and G‐2 were helical, more bluish in pigmentation than the wild type strain w...
Source: Journal of Basic Microbiology - November 1, 2017 Category: Microbiology Authors: Geeta G. Shirnalli, Manish S. Kaushik, Ajay Kumar, Gerard Abraham, Pawan K. Singh Tags: RESEARCH PAPER Source Type: research
Thermotolerant mycobiota of Israeli soils
Composition of thermotolerant mycobiota in the soil of Israeli deserts and northern territories was examined in spatiotemporal dynamics. A total of 165 species from 82 genera were isolated at 37 °C using the soil dilution plate method. Aspergilli (Aspergillus fumigatus and A. niger) and teleomorphic ascomycetes (Canariomyces notabilis, Chaetomium nigricolor, and Ch. strumarium) comprised the basic part of the thermotolerant communities. The desert communities remarkably differed from the northern communities by a much higher abundance of A. fumigatus and teleomorphic species, as well as by a lower abundance of A. niger ...
Source: Journal of Basic Microbiology - November 1, 2017 Category: Microbiology Authors: Isabella Grishkan Tags: RESEARCH PAPER Source Type: research
Metal transformation as a strategy for bacterial detoxification of heavy metals
This study clarified the capability of E. coli Z3 for indirect detoxification of heavy metals via the immobilization of metal ions into biologically unavailable species. (Source: Journal of Basic Microbiology)
Source: Journal of Basic Microbiology - November 1, 2017 Category: Microbiology Authors: Ashraf M. M. Essa, Mohamed A. Al Abboud, Sayeed I. Khatib Tags: RESEARCH PAPER Source Type: research
Zika virus: what we need to know?
Zika virus is one of the emerging viruses and is of significant threat to human health globally. It is a mosquito borne flavivirus similar to dengue, yellow fever, and West Nile viruses. It was reported about 5 decades ago and then it spreads to different parts of the world. Large outbreaks were reported on Yap Islands in 2007. Now it has gained wide attention globally by health communities. Major vector for virus transmission is Aedes aegypti mosquito. ZIKV infection is mostly asymptomatic but it is also responsible to cause mild influenza like illness to serious manifestations. There is no specific anti‐viral treatment...
Source: Journal of Basic Microbiology - November 1, 2017 Category: Microbiology Authors: Farakh Javed, Khanzadi N. Manzoor, Mubashar Ali, Irshad U. Haq, Abid A. Khan, Assad Zaib, Sobia Manzoor Tags: REVIEW Source Type: research
Morphological and cellular diversity of magnetotactic bacteria: A review
Magnetotactic bacteria (MTB) are getting much attention in the recent years due to the biomineralization in their magnetosomes (MS). MS are unique organelles that are bio‐mineralized due to MTB. MS contains nanosized crystal minerals of magnetite or greigite covered by bilayer lipid membrane, which are originated from cytoplasmic membrane (CM). MS are organized as an ordered chain into the cell which acts as a miniature compass needle. Furthermore, the biodiversity of MTB and their distribution is principally linked with the characteristics and growths of the MS. MTB are often considered as a part of the bacterial biomas...
Source: Journal of Basic Microbiology - November 1, 2017 Category: Microbiology Authors: Tariqul Islam, Changsheng Peng, Imran Ali Tags: REVIEW Source Type: research
Issue Information: Journal of Basic Microbiology. 10/2017
(Source: Journal of Basic Microbiology)
Source: Journal of Basic Microbiology - October 6, 2017 Category: Microbiology Tags: ISSUE INFORMATION Source Type: research
Cover: Journal of Basic Microbiology. 10/2017
Cover illustration:
Agseb1 affects the morphology formation of marinederived Aspergillus glaucus. Deletion of Agseb1 causes defects in conidial heads.
(Photo: Yuan Wu, East China University of Science and Technology, Shanghai, China) (Source: Journal of Basic Microbiology)
Source: Journal of Basic Microbiology - October 6, 2017 Category: Microbiology Tags: COVER PICTURE Source Type: research
Toxicity effects of AgZnO nanoparticles and rifampicin on Mycobacterium tuberculosis into the macrophage
This study showed that, AgZnONCs+Rifampicin has the most anti‐tubercular behavior with respect to the macrophages. (Source: Journal of Basic Microbiology)
Source: Journal of Basic Microbiology - October 1, 2017 Category: Microbiology Authors: Alireza Jafari, Saeedeh Jafari Nodooshan, Roya Safarkar, Farahnaz Movahedzadeh, Nader Mosavari, Aren Novin Kashani, Mahdi Dehghanpour, Morteza Kamalzadeh, Samira Rasouli Koohi, Sara Fathizadeh, Ali Majidpour Tags: RESEARCH PAPER Source Type: research
Diversity of methanogenic archaea in freshwater sediments of lacustrine ecosystems
About half of the global methane (CH4) emission is contributed by the methanogenic archaeal communities leading to a significant increase in global warming. This unprecedented situation has increased the ever growing necessity of evaluating the control measures for limiting CH4 emission to the atmosphere. Unfortunately, research endeavors on the diversity and functional interactions of methanogens are not extensive till date. We anticipate that the study of the diversity of methanogenic community is paramount for understanding the metabolic processes in freshwater lake ecosystems. Although there are several disadvantages o...
Source: Journal of Basic Microbiology - October 1, 2017 Category: Microbiology Authors: Folguni Laskar, Sumi Das Purkayastha, Aniruddha Sen, Mrinal K. Bhattacharya, Biswapriya B. Misra Tags: REVIEW Source Type: research
