Polyphenol oxidase from Pectobacterium atrosepticum: identification and cloning of gene and characteristics of the enzyme
In the present study, we attempted to elucidate if the harmful phytopathogenic bacteria of Pectobacterium genus (P. atrosepticum) possess the enzymes for oxidation of phenolic compounds. Polyphenol oxidase (laccase) activity was revealed in P. atrosepticum cell lysates. Using bioinformatic analysis, an ORF encoding a putative copper‐containing polyphenol oxidase of 241 amino acids with a predicted molecular mass of 25.9 kDa was found. This protein (named Pal1) shares significant level of identity with laccases of a new type described for several bacterial species. Cloning and expression of the pal1 gene and the analysi...
Source: Journal of Basic Microbiology - October 1, 2017 Category: Microbiology Authors: Vladimir Gorshkov, Nadezhda Tarasova, Natalia Gogoleva, Elena Osipova, Olga Petrova, Evgeny Kovtunov, Yuri Gogolev Tags: RESEARCH PAPER Source Type: research
Establishment of a markerless multiple ‐gene deletion method based on Cre/loxP mutant system for Bacillus pumilus
In this study, we established a Cre/loxP mutant recombination system (Cre/lox71‐66 system) for markerless gene deletion to facilitate our follow‐up rational genetic engineering to the strain Bacillus pumilus W3. This modified method uses two mutant loxP sites, which after recombination creates a double‐mutant loxP site that is poorly recognized by Cre recombinase, facilitating multiple gene deletions in a single genetic background. Two selected genes, cotA and sigF, were continuously knocked out and verified at different levels using this method. This method is simple and efficient and can be easily implemented for m...
Source: Journal of Basic Microbiology - October 1, 2017 Category: Microbiology Authors: Zheng ‐Bing Guan, Kai‐Qiang Wang, Yan Shui, Xiang‐Ru Liao Tags: METHOD PAPER Source Type: research
Electrochemically active iron (III) ‐reducing bacteria in coastal riverine sediments
This study expands our knowledge of the electrochemical characterization of the genus Anaerosinus. It is reasonable to expect that these isolates have potential applications in heavy metal bioremediation operations in natural environments. (Source: Journal of Basic Microbiology)
Source: Journal of Basic Microbiology - September 26, 2017 Category: Microbiology Authors: Shiling Zheng, Bingchen Wang, Ying Li, Fanghua Liu, Oumei Wang Tags: RESEARCH PAPER Source Type: research
Cr(VI) and lindane removal by Streptomyces M7 is improved by maize root exudates
Environmental mixed pollution by both organic and inorganic compounds are detected worldwide. Phytoremediation techniques have been proposed as ecofriendly methods for cleaning up polluted sites. Several studies have demonstrated enhanced dissipation of contaminants at the root‐soil interface through an increase in microbial activity caused by the release of plant root exudates (REs). The aim of this study was to evaluate the effectiveness for Cr(VI) and lindane removal by Streptomyces M7 cultured in a co‐contaminated system in presence of maize REs. Our results showed when REs were added to the contaminated minimal me...
Source: Journal of Basic Microbiology - September 21, 2017 Category: Microbiology Authors: Mar ía Z. Simon Sola, Daiana Pérez Visñuk, Claudia S. Benimeli, Marta Alejandra Polti, Analia Alvarez Tags: RESEARCH PAPER Source Type: research
Soil bacterial diversity changes in different broomcorn millet intercropping systems
In this study, characterization of different bacterial communities of monoculture and intercropping systems was achieved by deep sequencing. A total of 4684 operational taxonomic units were classified to the species level with good sampling depth and sequencing coverage. The abundance of Actinobacteria, Bacteroidetes, Proteobacteria, Acidobacteria, and Gemmatimonadetes varied at different growth stages and was related to growth of the intercropped plant. According to diversity analyses, Glycomyces, Aeromicrobium, Adhaeribacter, and Streptomyces were the dominant genera. In addition, we predicted functional gene composition...
Source: Journal of Basic Microbiology - September 21, 2017 Category: Microbiology Authors: Xiaoning Cao, Sichen Liu, Junjie Wang, Haigang Wang, Ling Chen, Xiang Tian, Lijun Zhang, Jianwu Chang, Lun Wang, Zhixin Mu, Zhijun Qiao Tags: RESEARCH PAPER Source Type: research
Combined genotyping strategy reveals structural differences between Aspergillus flavus lineages from different habitats impacting human health
In this study, 59 A. flavus isolates from four different habitats and 1 A. minisclerotigenes isolate were investigated. The isolates were identified and confirmed at the species level by the sequence analysis of a part of their calmodulin gene. Applying a combined analysis of UP‐PCR, microsatellite, and calmodulin sequence data, the four group of isolates formed separate clusters on the phylogenetic tree. Examining the distribution of mating type genes MAT1‐1 and MAT1‐2, a ratio of approximately 3:1 was determined, and no correlation was found between the carried mating type gene and the aflatoxin production capabili...
Source: Journal of Basic Microbiology - September 13, 2017 Category: Microbiology Authors: Nikolett Baranyi, S ándor Kocsubé, Daniela Jakšić Despot, Maja Šegvić Klarić, András Szekeres, Ottó Bencsik, Anita Kecskeméti, Palanisamy Manikandan, Beáta Tóth, László Kredics, Jamal M. Khaled, Naiyf S. Alharbi, Csaba Vágvölgyi, János Va Tags: RESEARCH PAPER Source Type: research
Issue Information: Journal of Basic Microbiology. 9/2017
(Source: Journal of Basic Microbiology)
Source: Journal of Basic Microbiology - September 4, 2017 Category: Microbiology Tags: ISSUE INFORMATION Source Type: research
Cover: Journal of Basic Microbiology. 9/2017
Cover illustration:
The formation of carbonates is seen with the majority of strains isolated from limestone habitats. Biominerals like calcit with different macromorphologies can be seen depending on the cultivated bacterial strain.
(Photo: Aileen Meier, Friedrich‐Schiller‐University, Jena, Germany) (Source: Journal of Basic Microbiology)
Source: Journal of Basic Microbiology - September 4, 2017 Category: Microbiology Tags: COVER PICTURE Source Type: research
Diversity of sediment ‐associated Planctomycetes in the Arabian Sea oxygen minimum zone
We examined the diversity of Planctomycetes in the sediment sample collected from an oxygen minimum zone (OMZ) in the southeast Arabian Sea. A 16SrRNA gene library was constructed using the forward primer specific for Planctomycetes and a universal reverse primer. The 237 sequences obtained were grouped into 130 operational taxonomic units, and the majority of them were clustered with phylum Planctomycetes (45.0%) and unclassified bacteria (27.0%). There were sequences that clustered with distantly separated monophyletic groups such as Latescibacteria (9%), Actinobacteria (6%), Proteobacteria (5%), and others (8%). Among P...
Source: Journal of Basic Microbiology - September 1, 2017 Category: Microbiology Authors: Chekidhenkuzhiyil Jasmin, Abdulaziz Anas, Balu Tharakan, Abdul Jaleel, Vipindas Puthiyaveettil, Saravanane Narayanane, Jovitha Lincy, Shanta Nair Tags: RESEARCH PAPER Source Type: research
Bacterial communities in ancient permafrost profiles of Svalbard, Arctic
Permafrost soils are unique habitats in polar environment and are of great ecological relevance. The present study focuses on the characterization of bacterial communities from permafrost profiles of Svalbard, Arctic. Counts of culturable bacteria range from 1.50 × 103 to 2.22 × 105 CFU g−1, total bacterial numbers range from 1.14 × 105 to 5.52 × 105 cells g−1 soil. Bacterial isolates are identified through 16S rRNA gene sequencing. Arthrobacter and Pseudomonas are the most dominant genera, and A. sulfonivorans, A. bergeri, P. mandelii, and P. jessenii as the dominant species. Other species belo...
Source: Journal of Basic Microbiology - September 1, 2017 Category: Microbiology Authors: Purnima Singh, Shiv M. Singh, Ram N. Singh, Simantini Naik, Utpal Roy, Alok Srivastava, Manfred B ölter Tags: RESEARCH PAPER Source Type: research
Rhizospheric fungi and their link with the nitrogen ‐fixing Frankia harbored in host plant Hippophae rhamnoides L.
Sea buckthorn (Hippophae rhamnoides L.) is a pioneer plant used for land reclamation and an appropriate material for studying the interactions of symbiotic microorganisms because of its nitrogen‐fixing root nodules and mycorrhiza. We used high‐throughput sequencing to reveal the diversities and community structures of rhizospheric fungi and their link with nitrogen‐fixing Frankia harbored in sea buckthorn collected along an altitude gradient from the Qinghai Tibet Plateau to interior areas. We found that the fungal diversities and compositions varied between different sites. Ascomycota, Basidiomycota, and Zygomycota ...
Source: Journal of Basic Microbiology - September 1, 2017 Category: Microbiology Authors: Xue Zhou, Lei Tian, Jianfeng Zhang, Lina Ma, Xiujun Li, Chunjie Tian Tags: RESEARCH PAPER Source Type: research
Candida albicans isolates from a single hospital show low phenotypical specialization
Candida albicans is the best‐studied opportunistic human pathogenic yeast species, and its virulence factors, susceptibility to antimycotics, the diversity of its physiological properties and the determinative factors of these traits are interesting from a clinical as well as from an evolutionary perspective. By applying statistical modeling for the phenotypical differences observed among a collection of 63 C. albicans isolates originating from different clinical care units, from a diverse group of patients with or without mycosis, collected in a Hungarian clinic, we found that (i) host‐related aspects like anatomical ...
Source: Journal of Basic Microbiology - September 1, 2017 Category: Microbiology Authors: Enik ő Boros, Walter P. Pfliegler, Renátó Kovács, Ágnes Jakab, László Majoros, Zoltán Barta, István Pócsi Tags: RESEARCH PAPER Source Type: research
Multilocus genotyping based species identification of entomopathogenic fungi of the genus Lecanicillium (=Verticillium lecanii s.l.)
Mitochondrial gene NADH dehydrogenase subunit 1 (nad1), β‐tubulin gene, and elongation factor 1‐alpha (tef) were used to characterize and to identify 42 Lecanicillum spp. isolates (former complex species Verticillium lecanii Zimm. Viegas) and to study the phylogenetic relationships in this group. Within the isolates under investigation, Lecanicillium muscarium was the most common species (about 70% of all isolates, collected on the different hosts, predominantly on the insects from the order Hemiptera). Based on nad1 sequencing four main molecular haplotypes were revealed. All four haplotypes have Holarctic origin. Mo...
Source: Journal of Basic Microbiology - August 8, 2017 Category: Microbiology Authors: Galina Mitina, Igor Kazartsev, Aleksandra Vasileva, Tapani Yli ‐Mattila Tags: RESEARCH PAPER Source Type: research
Issue Information: Journal of Basic Microbiology. 8/2017
(Source: Journal of Basic Microbiology)
Source: Journal of Basic Microbiology - August 4, 2017 Category: Microbiology Tags: Issue Information Source Type: research
Cover: Journal of Basic Microbiology. 8/2017
Cover illustration:
Ganoderma lucidum, the reishi mushroom used in traditional Chinese medicine, is a polypore occurring wordwide. It can be cultivated on sawdust and is used for its immunomodulatory metabolites.
(Photo: Elke‐Martina Jung, Friedrich‐Schiller‐University, Jena, Germany) (Source: Journal of Basic Microbiology)
Source: Journal of Basic Microbiology - August 4, 2017 Category: Microbiology Tags: Cover Picture Source Type: research
