In-vitro assessment of differential cytokine gene expression in response to infections with Egyptian classic and variant strains of highly pathogenic H5N1 avian influenza virus
In this study, expression of immune related genes (IL1b, IL4, IL6, IL8, IL10, IL18, IFNα and IFNγ) after infecting chicken macrophage cell line (HD11) and chicken peripheral blood Mononuclear cells (PBMC) with a classic and a variant strains was assayed using quantitative reverse-transcription real-time polymerase chain reaction assays (qRT-PCR). In HD11, the variant strain induced higher levels of IL1b and IL8 at 6hours post infection (hpi), IL4 at 24 / 48hpi and IFNα at 48hpi than the classic strain. Conversely, the classic strain induced about 10-fold increase of IFNγ at 24 and 48hpi and the virus replicated at high...
Source: International Journal of Veterinary Science and Medicine - October 18, 2015 Category: Veterinary Research Source Type: research

Effect of Arbutus pavarii, Salvia officinalis and Zizyphus Vulgaris on growth performance and intestinal bacterial count of broiler chickens
Publication date: Available online 4 December 2014 Source:International Journal of Veterinary Science and Medicine Author(s): A.A. Asheg , S.M. EL-Nyhom , K.M. Ben Naser , A.H. Kanoun , Y.M. abouzeed A study was conducted to determine the effect of three native plants from El-Jabal al ELAkhdar, (Libya) on performance and cecal coliform count of broiler chickens. A total of 1260 one-day-old male Cobb chickens were used in the experiment. The birds were assigned to 7 treatment groups (6 replicates per treatment). The dietary treatments included basal diet with no additive (control), and 6 other dietary treatments (A...
Source: International Journal of Veterinary Science and Medicine - December 5, 2014 Category: Veterinary Research Source Type: research

Iodoacetate and allogenous cartilage particles as models for arthritis induction in equine
Publication date: Available online 4 December 2014 Source:International Journal of Veterinary Science and Medicine Author(s): Ahmed Elmesiry , Magdi Seleim , David Cullis-Hill Experimental models of osteoarthritis (OA) have been widely developed in different animal species, because of the high incidence of osteoarthritis diseases in humans and animals. To date, no ideal OA animal model has been reported. The present study compare different osteoarthritis models to determine which one is suitable for inducing experimental equine OA. Fifteen donkeys were divided into three equal groups (n =5). The radio carpal joints ...
Source: International Journal of Veterinary Science and Medicine - December 5, 2014 Category: Veterinary Research Source Type: research

Diagnosis of Fasciola infection by SDS–PAGE eluted excretory secretory (ES) protein fractions using dot-ELISA
Publication date: Available online 2 December 2014 Source:International Journal of Veterinary Science and Medicine Author(s): M.A. Sabry , E.S. Taher , F.A. Narmeen , A.M. Mahgoub Fascioliasis is now recognized as an emerging zoonotic disease in Egypt. Diagnosis in suspected patients still needs some degree of accuracy. In the present study, three Fasciola gigantica execratory secretory (ES) protein bands of molecular weight (MW) ranging from 14 to 20kDa, 25 to 32kDa and 45 to 65kDa were eluted after fractionation of the parasite antigen using SDS–PAGE. The extracted kDa protein bands were concentrated and evalua...
Source: International Journal of Veterinary Science and Medicine - December 4, 2014 Category: Veterinary Research Source Type: research

The recognition of LpxC inhibitors as potential antibiotics could revolutionise the management of sepsis in veterinary patients if their unknown biological properties are widely evaluated in suitable animal models
Publication date: Available online 2 December 2014 Source:International Journal of Veterinary Science and Medicine Author(s): Saul Chemonges Current studies continue to show that UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetyl glucosamine deacetylase (LpxC) inhibitors such as pyridone methylsulfone hydroxymate 1 and 2a (LpxC-2) downregulate the lethal effects of sepsis initiated by multi-drug resistant Gram-negative bacteria (MDRGNB) by curtailing lipopolysaccharide (LPS) synthesis in murine models. Sepsis initiated by MDRGNB is a leading cause of shock and systemic inflammatory response syndrome (SIRS) in intensive care un...
Source: International Journal of Veterinary Science and Medicine - December 4, 2014 Category: Veterinary Research Source Type: research

Detection of immunoglobulins containing plasma cells in the thymus, bursa of Fabricius and spleen of vaccinated broiler chickens with Newcastle disease virus vaccine
In conclusion, mobilization of more Igs-positive cells in lymphoid tissues of broiler chickens is due to the effect of NDV vaccine as well as the advancement of age. (Source: International Journal of Veterinary Science and Medicine)
Source: International Journal of Veterinary Science and Medicine - December 4, 2014 Category: Veterinary Research Source Type: research

Effect of incremental levels of crude degummed canola oil on milk progesterone, plasma luteinizing and follicle stimulating hormones of primiparous Holstein–Friesian cows in a pasture-based system
Publication date: Available online 2 December 2014 Source:International Journal of Veterinary Science and Medicine Author(s): John R. Otto , Bunmi S. Malau-Aduli , Alexandra Rao , Iain J. Clarke , Aduli Enoch O. Malau-Aduli Dietary supplementation of lactating cows with fat can alter the profiles of key reproductive hormones and boost postpartum energy balance. However, published data under Australian pasture-based dairy production conditions are scanty and inconsistent. Therefore, the objective of this study was to determine whether dietary inclusion of crude degummed canola oil (CDCO) at incremental levels for e...
Source: International Journal of Veterinary Science and Medicine - December 4, 2014 Category: Veterinary Research Source Type: research

Evaluation and use of a serological assay for the detection of antibodies to Lawsonia intracellularis in swine
Publication date: Available online 3 December 2014 Source:International Journal of Veterinary Science and Medicine Author(s): Ronaldo L. Magtoto , Anuradha Vegi , Chong Wang , John K. Johnson , Sheela Ramamoorthy Porcine proliferative enteropathy (PPE) is a common and economically important gastro-intestinal disease of swine caused by the intracellular bacterium, Lawsonia intracellularis. Conventional tests to detect antibody responses to L. intracellularis include the immuno-peroxidase monolayer assay (IPMA), immuno-fluorescent antibody test (IFAT) and a lipopolysaccharide ELISA (LPS-ELISA). These tests are not c...
Source: International Journal of Veterinary Science and Medicine - December 4, 2014 Category: Veterinary Research Source Type: research

Co-circulation of avian influenza viruses in commercial farms, backyards and live market birds in Egypt
Publication date: Available online 4 December 2014 Source:International Journal of Veterinary Science and Medicine Author(s): H.A. Kaoud , H.A. Hussein , A.R. El-Dahshan , H.S. Kaliefa , M.A. Rohaim Cloacal and tracheal swab-samples were collected from commercial farms, backyards and live market birds (LBM) to identify the potential existence and genetic drifts of avian influenza subtypes (AI) H5 and H9 that are circulating among bird species in Egypt. The results revealed that, one sample out of 50 samples of chicken commercial farms was positive for the isolation of subtype H9N2 [KC699549, Influenza A virus: A/c...
Source: International Journal of Veterinary Science and Medicine - December 4, 2014 Category: Veterinary Research Source Type: research

Camel milk inhibits murine hepatic carcinogenesis, initiated by diethylnitrosamine and promoted by phenobarbitone
In conclusion, camel milk halted the progression of hepatocellular carcinoma. (Source: International Journal of Veterinary Science and Medicine)
Source: International Journal of Veterinary Science and Medicine - November 27, 2014 Category: Veterinary Research Source Type: research

Cutaneous adenocarcinoma in a desert tortoise (Gopherus agassizii)
This report describes the clinical and histopathological findings of a rare case of cutaneous adenocarcinoma in a 40-year-old desert tortoise. Surgical excision of the neoplasm improved the general health condition and locomotion of the tortoise although recurrence of the neoplasm had been recorded 1year post-surgery. (Source: International Journal of Veterinary Science and Medicine)
Source: International Journal of Veterinary Science and Medicine - November 4, 2014 Category: Veterinary Research Source Type: research

Attenuated virulence of pigment-producing mutant of Aeromonas veronii bv. sobria in HeLa cells and Nile tilapia (Oreochromis niloticus)
Publication date: June 2013 Source:International Journal of Veterinary Science and Medicine, Volume 1, Issue 1 Author(s): Said K. Abolghait , Mohamed F. Mohamed , Salah M. Aly , Aboubaker M. Garbaj , Ashraf A. Moawad Aeromonas species are potential water/foodborne pathogens, whereas Aeromonas veronii bv. sobria is one of the most virulent species to human and fish. Most current experimental evidence has publicized that suicide plasmid dependent IS1-element untargeted integration into A. veronii bv. sobria ATCC 9071T strain was recently used to generate brown pigment-producing and spontaneous pelleting (BP+SP+) mu...
Source: International Journal of Veterinary Science and Medicine - November 4, 2014 Category: Veterinary Research Source Type: research

Genotyping of Mycobacterium avium field isolates based on repetitive elements
Publication date: June 2013 Source:International Journal of Veterinary Science and Medicine, Volume 1, Issue 1 Author(s): A. El-Sayed , S. Natur , Nadra-Elwgoud M.I. Abdou , M. Salem , A. Hassan , M. Zschöck The economic and zoonotic importance of infections caused by Mycobacterium avium complex (MAC) strains in human, animals and birds are increasing. At present, few data are available about the genetic diversity of field isolates of M. avium subsp. hominissuis (MAH) and subspecies avium (MAA). The close relationship between human and swine isolates indicates a possible zoonotic role for such strains. In the p...
Source: International Journal of Veterinary Science and Medicine - November 4, 2014 Category: Veterinary Research Source Type: research

Molecular characterization of Mycobacterium avium subsp. paratuberculosis field isolates recovered from dairy cattle in Germany
Publication date: June 2013 Source:International Journal of Veterinary Science and Medicine, Volume 1, Issue 1 Author(s): Mohamed Salem , Saleh Natur , Amr A. El-Sayed , Abdulwahed Hassan , Georg Baljer , Michael Zschöck In the present work a total of 143 Mycobacterium avium subsp. paratuberculosis (Map) field isolates recovered from faeces of dairy cattle in Hessen State, Germany were investigated. Different Short Sequence Repeats (SSR), Mycobacterial Interspersed Repetitive Units (MIRU) and Variable Number Tandem Repeats (VNTR) were applied to classify these cattle-group field isolates into subgroups. When com...
Source: International Journal of Veterinary Science and Medicine - November 4, 2014 Category: Veterinary Research Source Type: research

Use of different Saccharomyces cerevisiae biotic forms as immune-modulator and growth promoter for Oreochromis niloticus challenged with some fish pathogens
Publication date: June 2013 Source:International Journal of Veterinary Science and Medicine, Volume 1, Issue 1 Author(s): Nermeen Abu-Elala , Mohamed Marzouk , Mohamed Moustafa The manipulation of intestinal bacterial flora through dietary supplementation of beneficial microbes is a new approach not only from the nutrition point of view but also to overcome the adverse effects of chemotherapeutants and lack of effective vaccines. The current study was performed to assess the role of Saccharomyces cerevisiae as a whole yeast cell (Probiotic), its extract (mannan-oligosaccharide - Prebiotic) and Pre-Probiotic mixture ...
Source: International Journal of Veterinary Science and Medicine - November 4, 2014 Category: Veterinary Research Source Type: research