Indian Journal of Medical Microbiology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Metagenomic next-generation sequencing in clinical microbiology
Jobin John Jacob, Balaji Veeraraghavan, Karthick VasudevanIndian Journal of Medical Microbiology 2019 37(2):133-140 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 18, 2019 Category: Microbiology Authors: Jobin John Jacob Balaji Veeraraghavan Karthick Vasudevan Source Type: research

Pulmonary Mycobacterium kyorinense disease: A case report and review of literature
We report here the first case of pulmonary infection due to Mycobacterium kyorinense in a 55-year-old hypertensive woman treated for pulmonary tuberculosis earlier on two occasions. She presented with productive cough, intermittent episode of left-sided chest pain, loss of appetite, low-grade fever, and breathlessness. Sputum cultures revealed non-tuberculous mycobacteria (NTM). She remained persistently symptomatic with sputum cultures positive for acid-fast bacilli even after 6 months of treatment. Hence, a 16SrRNA gene amplification and sequencing were done that revealed M. kyorinense. Based on the guidelines of the Ame...
Source: Indian Journal of Medical Microbiology - August 15, 2019 Category: Microbiology Authors: Rajagopalan Saranathan Chandrasekaran Padmapriyadarsini Gomathi Narayanan Sivaramakrishnan Bhavani K Perumal Silambuchelvi Kannayan Bency Joseph Narendran Gopalan Luke Elizabeth Hanna Source Type: research

Knee and the farm animals: An unusual presentation of Brucella melitensis masquerading as tuberculosis
We present a case of a rare manifestation of brucellosis i.e., septic arthritis of the knee joint associated with a lytic lesion of the proximal tibia. The patient belonged to a Brucella endemic country, and clinical features were of chronic reactive knee arthritis with synovial hypertrophy and effusion. Advanced diagnostic methods played a pivotal role in excluding the diagnosis of tuberculosis, and thus unnecessary administration of antitubercular therapy and initiating focused narrowed anti-Brucella management, achieving the goal of antimicrobial stewardship also. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 15, 2019 Category: Microbiology Authors: Raman Sardana Abhishek Vaish Aditya Merh Hena Butta Raju Vaishya Leena Mendiratta Source Type: research

Primary amoebic meningoencephalitis in an infant
Neetu Mittal, Lokesh Mahajan, Zahid Hussain, Parakriti Gupta, Sumeeta KhuranaIndian Journal of Medical Microbiology 2019 37(1):120-122 Primary amoebic meningoencephalitis is rare but fatal disease encountered in immunocompetent individuals. Here, we present a case of a previously healthy 8-month-old female child, who presented with features of meningoencephalitis of 2 days' duration. Rapidly moving trophozoites of amoeba were observed in cerebrospinal fluid, which were confirmed to be Naegleria fowleri on polymerase chain reaction. Broad-spectrum antimicrobial therapy with ceftriaxone, vancomycin, amphotericin ...
Source: Indian Journal of Medical Microbiology - August 15, 2019 Category: Microbiology Authors: Neetu Mittal Lokesh Mahajan Zahid Hussain Parakriti Gupta Sumeeta Khurana Source Type: research

Molecular confirmation of the circulating Bacillus anthracis during outbreak of anthrax in different villages of Simdega District, Jharkhand
Conclusion: The conventional and real-time PCR methods are suitable for both the clinical and the epidemiological practice. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 15, 2019 Category: Microbiology Authors: Manoj Kumar Kumari Seema Amber Prasad Ashok Kumar Sharma Banke Lal Sherwal Source Type: research

Eschar and IgM ELISA in the diagnosis of scrub typhus
This study aimed to determine the best diagnostic tool for the identification of scrub typhus and study the possible association between diagnostics and clinical characteristics. Patients with fever of ≤15 days admitted to the hospital satisfying the case definition of 47 kDa quantitative polymerase chain reaction (qPCR) positivity OR scrub typhus IgM ELISA positivity along with the presence of eschar OR Scrub typhus IgM ELISA positivity along with defervescence of fever within 72 h of initiation of specific therapy were recruited. Of the 116 patients satisfying the case definition, 47 kDa qPCR was positive in...
Source: Indian Journal of Medical Microbiology - August 15, 2019 Category: Microbiology Authors: Susmitha Karunasree Perumalla Sania Paul K P P. Abhilash Karthik Gunasekaran Winsley Rose Gowri Mahasampath Prakash John Jude Source Type: research

Combination antifungals as an effective means of salvage in paediatric leukaemia patients with invasive fungal infections
Jagdish Prasad Meena, Aditya Kumar Gupta, Manisha Jana, Rachna SethIndian Journal of Medical Microbiology 2019 37(1):109-112 Invasive fungal infections (IFIs) are an important cause of morbidity and mortality in paediatric leukaemias. Antifungal combinations to treat these patients are being explored. Fourteen children with leukaemias and IFIs were treated with a combination of antifungal agents at our centre. The first antifungal was amphotericin-B in 13 children and voriconazole in one child. In view of no improvement and clinical deterioration, in nine patients, voriconazole was added as the second antifungal agent and...
Source: Indian Journal of Medical Microbiology - August 15, 2019 Category: Microbiology Authors: Jagdish Prasad Meena Aditya Kumar Gupta Manisha Jana Rachna Seth Source Type: research

Use of TaqMan Array card for the detection of respiratory viral pathogens in children under 5 years old hospitalised with acute medical illness in Ballabgarh, Haryana, India
Bharti Gaur, Siddhartha Saha, A Danielle Iuliano, Sanjay K Rai, Anand Krishnan, Seema Jain, Brett Whitaker, Jonas Winchell, Renu B Lal, Shobha BroorIndian Journal of Medical Microbiology 2019 37(1):105-108 Historical specimens collected from hospitalized children were tested for the following 13 viruses: influenza A and B; respiratory syncytial virus (RSV); parainfluenza viruses 1–3; human metapneumovirus; rhinovirus; coronaviruses 229E, OC43, NL63 and HKU1 and Adenovirus using monoplex real-time reverse transcriptase polymerase chain reaction (rRT-PCR). They were retested using TaqMan Array Card (TAC), a mic...
Source: Indian Journal of Medical Microbiology - August 15, 2019 Category: Microbiology Authors: Bharti Gaur Siddhartha Saha A Danielle Iuliano Sanjay K Rai Anand Krishnan Seema Jain Brett Whitaker Jonas Winchell Renu B Lal Shobha Broor Source Type: research

Distribution of human leucocyte antigen-C in HIV-1-infected patients in Mumbai, India
Nikhil S Bardeskar, Vijay R Chavan, Swati Ahir-Bist, Padmaja Samant-Mavani, Preeti R Mehta, Jayanti Mania-PramanikIndian Journal of Medical Microbiology 2019 37(1):102-104 HIV pathogenesis is known to be highly influenced by host genetic factors, such as human leucocyte antigens (HLAs) HLA-A and HLA-B. However, the role of HLA-C remains largely unexplored. We evaluated HLA-C distribution in 186 HIV-1-infected individuals and compared them to ethnically matched data derived from the Allele Frequency Net Database using Chi-square test with Fisher's exact two-tailed test. The frequency of HLA-C*05 and H...
Source: Indian Journal of Medical Microbiology - August 15, 2019 Category: Microbiology Authors: Nikhil S Bardeskar Vijay R Chavan Swati Ahir-Bist Padmaja Samant-Mavani Preeti R Mehta Jayanti Mania-Pramanik Source Type: research

Sonication of antibiotic loaded cement spacers: A valuable technique for detection of infection persistence in two-stage revision for infected joint arthroplasty
Sujeesh Sebastian, Rajesh Malhotra, Benu Dhawan, Vishnubhatla Sreenivas, Arti Kapil, Rama ChaudhryIndian Journal of Medical Microbiology 2019 37(1):99-101 We evaluated the diagnostic utility of sonication of antibiotic loaded cement spacers comparing with periprosthetic tissue cultures for the detection of persisting infection in 14 patients undergoing staged procedures. Sonication improved microbial detection of intraoperative cultures from 14.2% to 28.5% (P = 0.481). Routine sonication of spacers is recommended. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 15, 2019 Category: Microbiology Authors: Sujeesh Sebastian Rajesh Malhotra Benu Dhawan Vishnubhatla Sreenivas Arti Kapil Rama Chaudhry Source Type: research

Relative potency of different generic brands of meropenem, colistin and fosfomycin: Implications for antimicrobial therapy and antimicrobial formulary
Parijat Das, Bishwaranjan Jana, Kingshuk Dhar, Gaurav Goel, Sanjay Bhattacharya, Mammen ChandyIndian Journal of Medical Microbiology 2019 37(1):95-98 There is a need of a relatively simple and inexpensive method for the determination of relative potency of various generic brands of antibiotics in comparison to original products. The current study describes an agar diffusion method which can be performed in any microbiology laboratory, is cheap (costs $2 per test) and its results can be available after overnight incubation. The results show that neither all generics are reliable nor are all generic antibiotics of poor qual...
Source: Indian Journal of Medical Microbiology - August 15, 2019 Category: Microbiology Authors: Parijat Das Bishwaranjan Jana Kingshuk Dhar Gaurav Goel Sanjay Bhattacharya Mammen Chandy Source Type: research

Bad bug, no test: Tigecycline susceptibility testing challenges and way forward
Chaitra Shankar, Agila Kumari Pragasam, Balaji Veeraraghavan, Anushree AmladiIndian Journal of Medical Microbiology 2019 37(1):91-94 Tigecycline is a reserve antibiotic increasingly used for the treatment of multidrug-resistant bacteria, especially Klebsiella pneumoniae and Acinetobacter baumannii. At present, there are concerns regarding the testing and interpretation of tigecycline susceptibility to bugs such as K. pneumoniae and A. baumannii, which limit clinicians in appropriate usage. Use of appropriate method for testing such as broth microdilution is essential. In addition, tigecycline susceptibility testing is a c...
Source: Indian Journal of Medical Microbiology - August 15, 2019 Category: Microbiology Authors: Chaitra Shankar Agila Kumari Pragasam Balaji Veeraraghavan Anushree Amladi Source Type: research

Colistin-sparing approaches with newer antimicrobials to treat carbapenem-resistant organisms: Current evidence and future prospects
Balaji Veeraraghavan, Agila Kumari Pragasam, Yamuna Devi Bakthavatchalam, Shalini Anandan, Subramanian Swaminathan, Balasubramanian SundaramIndian Journal of Medical Microbiology 2019 37(1):72-90 Antimicrobial resistance is on the rise across the globe. Increasing incidence of infections due to carbapenem resistance organisms is becoming difficult to treat, due to the limited availability of therapeutic agents. Very few agents such as colistin, fosfomycin, tigecycline and minocycline are widely used, despite its toxicity. However, with the availability of novel antimicrobials, beta-lactam/beta-lactamase inhibitor-based an...
Source: Indian Journal of Medical Microbiology - August 15, 2019 Category: Microbiology Authors: Balaji Veeraraghavan Agila Kumari Pragasam Yamuna Devi Bakthavatchalam Shalini Anandan Subramanian Swaminathan Balasubramanian Sundaram Source Type: research

Microbiological profile and outcomes of two-stage revision hip arthroplasty
Conclusion: The outcomes of the study justify a two-stage revision arthroplasty for PJI of the hip. The use of ESR and CRP as screening tests for the success of debridement has value - but should be interpreted with caution. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 15, 2019 Category: Microbiology Authors: TD Hariharan VJ Chandy Jerry George Ajit Joseph Mathew John Premnath Agila Kumari Pragasam John Jude Prakash Anil Thomas Oomen Pradeep Mathew Poonnoose Source Type: research

Evaluation of dried blood spots as a feasible alternative to plasma for the detection and quantification of hepatitis c virus in a tropical setting: A pilot study
Conclusions: DBS can be used for sampling patients from distant resource-limited settings as an alternative to plasma for HCV RNA estimation. Larger studies are required to evaluate the feasibility of DBS in the Indian subcontinent, especially for HCV core antigen estimation. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 15, 2019 Category: Microbiology Authors: Jai Ranjan Suresh Ponnuvel Gnanadurai John Fletcher Raghavendran Anantharam Kalaivani Radhakrishnan Visalakshi Jeyaseelan Priya Abraham Source Type: research